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This work illustrates how hyperspectral imaging, carried out from a moving ground vehicle, can be used under field conditions for assessing grape composition and evaluating its spatio-temporal variability within a vineyard. To analyse the distribution of the grape composition parameters, measurements were carried out at four different dates during the ripening season, at which total soluble solids (TSS) and anthocyanin concentrations analyses were also performed. Prediction models were generated for the two parameters, yielding 10-fold cross validation determination coefficients (R2) of 0.92 for TSS and 0.72 for anthocyanin concentrations (RMSEs of 1.444 °Brix and 0.272 mg/g berry, respectively). The results were used for mapping the evolution and distribution of grape composition in time-four dates-and space-the vineyard plot, illustrating the suitability of this technology for the in-field monitoring of the vineyard.