In-vivo intramuscular collagen turnover in Pectoralis major in two broiler strains in relation to woody breast myopathy
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A study was conducted to understand in vivo soluble (S-) and insoluble (I-) collagen fractional synthesis and degradation rates (FSR and FDR) in Pectoralis major (breast muscle) using stable isotope flooding method in two commercial strains of broilers: Strain A and Strain B during grow-out cycle (d 21, d 28, d 35, d 42, and d 57). Birds (n=10) were infused with 1-13C proline as tracer amino acid at each sampling age, and isotopic enrichment 13C-to-12C ratio for muscle and plasma samples were measured and then FSR and FDR were calculated on percent per hour (%/h) basis. The FSR and FDR decreased over the age of birds for both the strains for S- and I- collagen; however, Strain B had higher FSR or FDR values until d 42. The FSR and FDR values at d 21 for Strain B was 0.196 and 0.194%/h whereas it was 0.129%/h and 0.127%/h for Strain A. The FSR and FDR values dropped to almost undetectable range on %/h basis by d 56 (0.001 and 0.0001%/h for Strain B). Total collagen was significantly higher (P<0.05) for Strain B than Strain A throughout the grow-out period (Strain A: 4.50 vs Strain B: 12.12 μg/mg by d 56) which was evident in micrograph results. Strain B exhibited higher (P<0.05) subjective WB scores. The findings suggest that there is marked age related changes in collagen metabolism for both S- and I- collagen protein in P. major for both strains, however Strain B is more vulnerable to myopathy condition due to increased synthesis of collagen occurring at an earlier age.