Effect of a grazing event in mRNA expression of genes involved in energy metabolism in dairy cows
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Eighteen Holstein cows (9 multiparous (MUL) and 9 primiparous (PRI), days in milk=73±7; milk yield=26±3 kg) that grazed together in a vegetative oat pasture and received supplementation after pm milking (6 kg DM/day of mixed ration 55:45 forage to concentrate ratio, dry matter (DM) basis) were used in a randomized complete block design to assess the metabolic status (glucose, non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB)) and mRNA expression of target genes (pyruvate carboxylase (PC), cytosolic phosphoenolpyruvate carboxykinase (PEPCKc), citrate synthase (CS), propionyl-CoA carboxylase (PROP-CoAC), ATP synthase 5B (ATP5B), carnitine palmitoyl-transferase (CPT1A)) in response to the first daily grazing event (FGE). Glucose and NEFA concentrations decrease (P<0.050) while BHB increased (P<0.001) from pre- to post-FGE in both parities, while changes (P<0.05) of relative mRNA expression of PC, CS, ATP5B and CPT1A genes were only observed in MUL suggesting differences in hepatic molecular adaptation between parities.