A study of marking fluid (MF) of Indian male leopard has been undertaken as a continuation of the earlier work on tiger and cheetah pheromones. The behavioural observations reveal that MF is presumably the primary means of communication in leopard. The chemical analyses reveal the presence of acetic, propionic, isobutyric, butyric, isovarleric, valeric, isohexanoic, hexanoic, isoheptanoic, heptanoic, isooctanoic, octanoic and nonanoic acid in the acidic fraction of steam distillate of MF. Amines like etylenediamine, putrescine, cadaverine and phenylethylamine, dimethylamine and trimethylamine were also detected in the basic fraction of MF. The amount of lipid extracted from MF is 1.15 mg/ml.