Little is known about the status of Puma concolor populations in Central and South America. Due to this reason, the present study contributes to the knowledge on puma populations providing information on abundance and density in Sierra Nanchititla, Mexico, as well as comparing recorded values against those obtained from zones of high and low concentration of camera traps. The sampling was carried out during a period of 30 months, which were divided into 3-month independent blocks. For the 8 first blocks, ten camera traps were set in an area where the separation among cameras was 1.6 km, and in the 2 last blocks 17 camera traps were set in an area where the separation was 4.6 km. The abundance was estimated between 2 and 5 individuals for the area with the highest concentration of cameras, lower than the abundance obtained in the area with the lowest concentration of cameras, i.e., 6 individuals. The density of the area with the highest camera concentration had a variation between 2.74 and 6.86 individuals/100 km2 in an effective survey area of 72.8 km2 and for the area of lowest concentration it was 1.21 individual/100 km2 in an effective survey area of 492.6 km2.