This study represents the initial attempt to evaluate the effect of egg protein-bound amino acid (i.e., vitellogenin-derived PAAs) content on embryo/larva success in Dentex dentex. Viable eggs were obtained from a single female (i.e., intra-individual analysis) in captivity. Selected embryo/larva viability parameters (the egg floating rate, the hatching rate, and the larval survival rate at days 0 to 5 post-hatch) were determined. The egg batches were categorised in three groups (high-, medium-, and low-quality batches) based on the magnitude of their hatching rates. The protein-bound amino acid content of biometrically similar egg batches of varying quality was analysed. The content values for 12 protein-bound amino acids (i.e., essential PAAs: Thr, Met, Arg, Ile, Val, Phe, His; non-essential PAAs: Ala, Glu, Gly, Asp, and Ser) were significantly correlated with the embryo/larva viability parameters in an analysis performed in terms of their ratios. The egg protein-bound amino acid content was significantly correlated with the dentex larval survival rate at 3 and 5 days post-hatch, a critical period of survival that is problematic during the propagation of this species in captivity. The results of this study also offer statistical evidence for the effects of the interrelationships of the egg protein-bound amino acids on the relationships between the quantitative composition of the protein-bound amino acids and the viability parameters.
Molecular alteration of three forms of vitellogenins and their product yolk proteins during oocyte growth and maturation in grey mullet (Mugil cephalus).
Fishes of the northeastern Atlantic and the MediterraneanVol. 2 pp.
883-907. United Nations Educational Scientific and Cultural OrganizationParis.
Egg quality determinants in finfish: the role of overripening with special reference to the timing of stripping in the Atlantic halibut, Hippoglossus hippoglossus.
J. World Aquac. Soc.2513-21.
Development of broodstock diets for the European sea bass (Dicentrarchus labrax) with special emphasis on the importance of n − 3 and n − 6 highly unsaturated fatty acid to reproductive performance.
Yolk formation and degradation during oocyte maturation in seabream Sparus aurata: involvement of two lysosomal proteinases.
Influence of egg composition on fertilization rate in sablefish (Anoplopoma fimbria). In:
Incubation of Fish: Biology and Techniques pp.
71-73. International Congress on the Biology of Fish, University of British ColumbiaCanada.
Two forms of vitellogenin, yielding two distinct lipovitellins, play different roles during oocyte maturation and early development of barfin flounder, Verasper moseri, a marine teleost that spawns pelagic eggs.
Utilization of free amino acids, yolk proteins and lipids in developing eggs and yolk-sac larvae of walleye pollock Theragra chalcogramma.
Isolation and characterization of polymorphic microsatellite markers in Pagellus bogaraveo, and cross-species amplification in Sparus aurata and Dicentrarchuslabrax.
Conserved and variant molecular and functional features of multiple egg yolk precursor proteins (vitellogenins) in white perch (Morone americana) and other teleosts.
Lipovitellins derived from two forms of vitellogenin are differentially processed during oocyte maturation in haddock (Melanogrammusaeglefinus).
J. Exp. Zool.291(1)
Utilization of free amino acids related to energy metabolism of developing eggs and larvae of lemon sole Microstomus kitt reared in the laboratory.
Mar. Ecol. Prog. Ser.88195-205.
SamaeeS.-M. (2010) Quantitative composition of egg protein lipid fatty acid and free amino acid in common dentex (Dentex dentex L.) and their relations to viability and larval development. Ph.D. Dissertation. Department of Organismic Biology University of Salzburg Austria.
Quantitative composition of vitellogenin-derived yolk proteins and their effects on viability of embryos and larvae of common dentex (Dentex dentex), a marine pelagophil teleost.
J. Exp. Zool.311A504-520.
Evaluation of quantitative importance of egg lipids and fatty acids during embryos and larvae development in marine pelagophil teleosts: with an emphasis on Dentex dentex.
Embryo and larva development in Common Dentex (Dentex dentex), a pelagophil teleost: the compositions of eggs free amino acids and their interrelations.
Multiple vitellogenins (Vgs) in mosquitofish (Gambusia affinis): identification and characterization of three functional Vg genes and their circulating and yolk protein products.
Incorporation and utilization of multiple forms of vitellogenin and their derivative yolk proteins during vitellogenesis and embryonic development in the mosquitofish, Gambusia affinis.
Molecular characterization of three forms of vitellogenin and their yolk protein products during oocyte growth and maturation in red seabream (Pagrus major), a marine teleost spawning pelagic eggs.
Mol. Reprod. Dev.73719-736.
Identification of two forms of vitellogenin-derived phosvitin and elucidation of their fate and roles during oocyte maturation in the barfin flounder, Verasper moseri.
Controlled accumulation of multiple vitellogenins into oocytes during vitellogenesis in the barfin flounder, Verasper moseri.
Relation of carp offspring quality on age of females, on amino acid content in eggs, and addition of amino acids to water at the beginning of development. In:
Ekologicheskaya fiziologiya ryb (Tezizy Dokl. Vsesoyuzn. Konf. Po Ekologicheskoj Fiziologii Ryb Moskva 1973) (in Russian) pp.
93-95. Minist. Rybnogo Khoz. SSSRMoscow.