Functional morphology of the gut of the tropical house gecko Hemidactylus mabouia (Squamata: Gekkonidae)

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The purpose of this study was to characterize morphophysiological aspects of the gut of the gecko Hemidactylus mabouia, a predator species of tiny arthropods. Fourteen adult specimen of the gecko H. mabouia were euthanized and fragments of their small and large intestines were collected and processed according to routine methods for anatomical, topological, histological and histochemical analyses. Histological sections were stained with toluidine blue or submitted to techniques for identification of argyrophil and argentaffin endocrine cells, glycoconjugates and alkaline phosphatase activity. The small intestine of H. mabouia is much more extensive and convolute than the large intestine. There are subtle regional differences along the small intestine, as the tubule diameter and height of the inner folds noticeably decrease from the proximal toward the distal segment. There is no caecum between the small and large intestines and the abrupt change in the caliber marks the transition of the small intestine into the large intestine. The large intestine consists of a very dilated proximal segment followed by a short distal segment. The villi are absent, but the tall folds in the internal covering of the small intestine constitute important amplifier structures of the digestive and absorptive area. No mucosal or submucosal glands were observed along the intestine. The epithelial lining of the entire intestine is simple columnar with enterocytes, mucus-secreting cells and endocrine cells. The enterocytes are abundant in the small intestine and the mucus-secreting cells are abundant in the large intestine, which reflects the functional role of these organs. In sum, H. mabouia has small intestine that is longer than the large intestine, which is consistent with the species being a carnivorous reptile.

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References

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Figures

  • Gross anatomy of the gut of H. mabouia. (A) Small intestine with its regions proximal (pr), median (md) and distal (ds), and large intestine with its segments proximal colon (pc) and distal colon (dc). (B) Internal coating of the proximal small intestine. (C) Internal coating of the median small intestine. (D) Internal coating of the small intestine (si) – large intestine (li) transition. (E) Internal coating of the proximal colon (pc) – distal colon (dc) transition. Arrow: fold; asterisk: valve-like projection; p: pancreas; s: stomach. This figure is published in colour in the online version.

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  • Scanning electron microscopy of the small intestine of H. mabouia. (A) Internal covering of the median region. (B) Internal covering of the proximal region. (C) Epithelial lining of the distal region. (D) Epithelial lining of the distal region. Arrow: fold; arrow-head: valve in the central lymph vessel; ec: enterocytes; mc: mucus-secreting cells.

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  • Scanning electron microscopy of the large intestine of H. mabouia. (A) Internal covering of the proximal colon. (B) Epithelial lining of the proximal colon. (C) Epithelial lining of the distal colon. (D) Epithelial lining of the distal colon. (E) Protozoa (black arrow-head) on the internal surface of the proximal colon. (F) Bacteria (white arrow-head) on the internal surface of the distal colon. Arrows: folds; asterisk: cells with retracted border; mc: mucus-secreting cells.

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  • Histology and histochemistry of the small intestine of H. mabouia. (A) Transversal section of the wall of the proximal region (Toluidine Blue). (A′) Detail of the muscular tunic showing a nervous ganglion (rectangle) and a mast cell (circle). (B) Transversal section of the wall of the median region (PAS). (C) Mucosa tunic of the proximal region (toluidine blue). (D) Mucosa tunic of the distal region (PAS). (E) Epithelial lining of the proximal region (alkaline phosphatase). (F) Epithelial lining of the distal region (alkaline phosphatase). (G) Mucosa tunic of the distal segment (alcian blue pH 2.5). (H) Epithelial lining of the proximal region (PAS-alcian blue). Arrow: fold; arrow-head: brushborder; asterisk: central lymphatic vessel; bm: basal membrane; ec: enterocyte; gc: goblet cells; mc: mucosa; mm: muscularis mucosae; ms: muscularis; sm: submucosa; sr: serosa; stippled circle: intraepithelial lymphocyte; stippled rectangle: mitotic cell division. This figure is published in colour in the online version.

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  • Enteroendocrine cells of the gut of H. mabouia. (A) Argyrophil cell (stippled circle) in the proximal small intestine. (B) Argentaffin cell (arrow) in the proximal colon. (C) Argentaffin cell (arrow) in the distal colon. This figure is published in colour in the online version.

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  • Histology and histochemistry of the large intestine of H. mabouia. (A) Longitudinal section of the wall of the proximal colon (toluidine blue). (B) Transversal section of the wall of the distal colon (toluidine blue). (C) Epithelial lining of the distal colon (alcian blue pH 2.5). (D) Epithelial lining of the distal colon (PAS). (E) Epithelial lining of the proximal colon (PAS-alcian blue). (F) Epithelial lining of the proximal colon (alkaline phosphatase). Arrow: crypt-like depression; arrow-head: mastocyte; circle: intraepithelial lymphocyte; ec: enterocyte; ep: epithelium; la: lymphatic agglomerate; lp: lamina propria; mc: mucus-secreting cell; mm: muscularis mucosae; ms: muscularis; sm: submucosa; sr: serosa. This figure is published in colour in the online version.

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