Genetic relatedness provides support for a species complex of myxosporeans infecting the Indian major carp, Labeo rohita

in Animal Biology
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Myxozoans are an economically important group of microscopic metazoan parasites of fish. The myxozoan species Thelohanellus filli infects commercially important freshwater fish both in wild and in cultured habitat. This parasite causes gill hemorrhagic disease and significant damage to the respiratory surface of the infected fish. In the present study, the 18S rDNA gene sequence of morphologically identified T. filli infecting the gill lamellae of the Indian major carp, Labeo rohita, was characterized. This revealed that we are actually dealing with a species complex containing T. bifurcata, T. jiroveci and T. seni. Phylogenetically, T. filli clustered with other myxozoan parasites, with the species most closely related to T. filli having 96 to 97% sequence similarity. The intraspecific variation demonstrated in this study points towards the importance of newer approaches to facilitate reassessment of taxa and detecting species complexes and cryptic species.

Genetic relatedness provides support for a species complex of myxosporeans infecting the Indian major carp, Labeo rohita

in Animal Biology

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References

AndreeK.B.SzékelyC.MolnárK.GresoviacS.J.HedrickP.P. (1999) Relationships among member of the genus Myxobolus (Myxozoa: Bivalvidae) based on small subunit ribosomal DNA sequences. J. Parasitol.8568-74.

AtkinsonS.D.Bartošová-SojkováP.WhippsC.M.BartholomewJ.L. (2015) Approaches for characterising Myxozoan species. In: OkamuraB. (Eds.) Myxozoan Evolution Ecology and Development pp.  111-123. Springer International PublishingSwitzerland.

Bartošová-SojkováP.HrabcováM.PeckováH.PatraS.KodádkováA.JurajdaP.TymlT.HolzerA.S. (2014) Hidden diversity and evolutionary trends in malacosporean parasites (Cnidaria: Myxozoa) identified using molecular phylogenetics. Int. J. Parasitol.44565-577.

BasuS.ModakB.K.HaldarD.P. (2006) Synopsis of the Indian species of the genus Thelohanellus Kudo, 1933 along with description of Thelohanellus disporomorphus sp. n. J. Parasit. Dis. Appl. Anim. Biol.1581-94.

BasuS.ModakB.K.HaldarD.P. (2015) Biological diversity of Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) parasitizing freshwater fishes of Indian subcontinent. J. Env. Sociobiol. (online)026-27http://www.i-scholar.in/index.php/JESEBA/article/view/62155.

FialaI. (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. Int. J. Parasitol.361521-1534.

HartikainenH.GruhlA.OkamuraB. (2014) Diversification and repeated morphological transitions in endoparasitic cnidarians (Myxozoa: Malacosporea). Mol. Phylogenet. Evol.76261-269.

KalavatiC.NandiN.C. (2007) Handbook of Myxosporidean Parasites of Indian Fishes. ZSIIndia, Kolkata293 pp.

KaurH. (2014) A rewiew article – Myxozoan infestation in freshwater fishes in wetlands and aquaculture in Punjab (India). Adv. Anim. Vet. Sci.2488-502.

KaurH.AttriR. (2015) Prevalence and tissue specificity of Myxobolus saugati Kaur and Singh, 2011 (Myxozoa; Myxosporea; Bivalvulida) causing dermal myxoboliosis in wild and cultured Indian major carps in Punjab. Species1514-18.

KaurH.KatochA. (2014) Gill disease caused by Thelohanellus bifurcata Basu and Haldar, 1999 a pathogenic myxozoan parasite in cultured Indian carp, Labeo rohita (Hamilton, 1822) in Punjab, India. J. Anim. Health. Prod.219-24.

KaurH.DarS.A.KatochA. (2014a) Thelohanellus dykovi sp. nov. (Myxozoa: Bivalvulidae), a pathogenic gill parasite in cultured Indian major carp, Labeo rohita (Hamilton 1822) in Punjab (India). Species1024-30.

KaurH.KatochA.GuptaM. (2014b) Thelohanellus filli sp. n., a pathogenic myxosporean infecting gills of cultured carp, Labeo rohita (Hamilton 1822) in Punjab, India. Species1031-38.

KentM.L.AndreeK.B.BartholomewJ.L.El-MatbouliM.DesserS.S.DevlinR.H.FeistS.W.HedrickR.P.HoffmannR.W.KhattraJ.HallettS.L.LesterR.J.G.LongshawM.PalenzeulaO.SiddallM.E.XiaoC. (2001) Recent advances in our knowledge of the Myxozoa. J. Euk. Microbiol.48395-413.

KimuraM. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol.16111-120.

LiuY.WhippsC.M.LiuW.S.ZengL.B.GuZ.M. (2011) Supplemental diagnosis of a myxozoan parasite from common carp Cyprinus carpio: synonymy of Thelohanellus xinyangensis with Thelohanellus kitauei. Vet. Parasitol.178355-359.

LomJ.DykováI. (1992) Protozoan Parasites of Fishes. Development in Aquaculture and Fisheries pp.  159-235. ElsevierAmsterdam.

LomJ.DykováI. (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitologica531-36.

MolnárK. (1994) Comments on the host, organ and tissue specificity of fish myxosporeans and on the types of their intrapiscine development. Parasitologica Hungarica275-20.

MolnárK. (2002) Site preference of fish myxosporean in the gill. Dis. Aqua. Org.78147-153.

MondalA.BanerjeeS.PatraA.AdikesavaluH.RamuduK.R.DashG.JoardarS.N.AbrahamT.J. (2014) Molecular and morphometric characterization of Thelohanellus caudatus (Myxosporea: Myxobolidae) infecting the caudal fin of Labeo rohita (Hamilton). Protistology841-52.

RonquistF.HuelsenbeckJ.P. (2003) Bayesian phylogenetic inference under mixed models. Biogeosciences191572-1574.

SeoJ.S.JeonE.J.KimM.S.WooS.H.KimJ.D.JungS.H.ParkM.A.JeeB.Y.KimJ.W.KimY.C.LeeE.H. (2012) Molecular identification and real-time quantitative PCR (qPCR) for rapid detection of Thelohanellus kitauei, a myxozoan parasite causing intestinal giant cystic disease in the Israel carp. Korean J. Parasitol.50103-111.

ShinS.P.KimJ.H.ChorescaC.Jr.HanJ.E.JunJ.W.ParkS.C. (2013) Molecular identification and phylogenetic characterisation of Thelohanellus kitauei. Acta Vet. Hungarica6130-35.

SinghR.KaurH. (2012a) Biodiversity of myxozoan parasites infecting freshwater fishes of three main wetlands of Punjab, India. Protistology779-89.

SinghR.KaurH. (2012b) Thelohanellus (Myxozoa: Myxosporea: Bivalvulida) infections in major carp fish from Punjab wetlands (India). Protistology7178-188.

SinghR.KaurH. (2012c) Myxosporean species of the genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosprea: Bivalvulida) from freshwater fishes of Punjab wetlands, India. Protistology7208-217.

SinghR.KaurH. (2012d) Two new Thelohanellus Kudo, 1933 (Myxozoa: Myxosprea: Bivalvulida) infecting fins of major carps in wetlands of Punjab. Trends Parasitol. Res.134-43.

SinghR.KaurH. (2014) Two new and two already known species of genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) infecting Indian major carp fishes in Punjab wetlands (India). J. Parasitic Dis.3849-60.

SinghR.KaurH. (2015) Two new and one already known species of the genus Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida) parasitizing fresh water fishes in wetlands of Punjab, India. Biologia7085-93.

Sitjà-BobadillaA. (2008) Fish immune response to myxozoan parasites. Parasite15420-425.

Sitjà-BobadillaA.Alvarez-PelliteroP. (1993) Pathologic effects of Sphaerospora dicentrarchi Sitjà-Bobadilla and Alvarez Pellitero, 1992 and S. testicularis Sitjà-Bobadilla and Alvarez Pellitero, 1990 (Myxosporea: Bivalvulida) parasitic in the Mediterranean sea bass Dicentrarchus labrax L. (Teleostei: Serranidae) and the cell-mediated immune reaction: a light and electron microscopy study. Parasitol. Res.79119-129.

TamuraK.PetersonD.PetersonN.StecherG.NeiM.KumarS. (2013) MEGA6: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol.282731-2739.

ZhangJ.Y.GuZ.M.KalavatiC.EirasJ.C.LiuY.GuoQ.Y.MolnarK. (2013) A synopsis of the species of Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Bivalvulida). Sys. Parasitol.86235-256.

ZhuY.T.LuH.D.CaiS.J. (2012) Redescription of Thelohanellus wuhanensis Xiao et Chen (Myxozoa, Myxosporea) infecting allogynogenetic crusian carp (Carassius auratus gibelio) and phylogenetic analysis based on 18S rDNA sequence. Acta Zootaxa Sinica37681-686.

Figures

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    Fresh spores of gill infecting Thelohanellus filli (1000×) from Indian major carp, Labeo rohita.

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    Line drawing of spores of Thelohanellus filli showing frontal view (A) and sutural view (B).

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    Phylogenetic tree generated by Maximum Likelihood of the 18S rRNA gene sequences of Thelohanellus filli (Accession number KR340464) and other related species. Bootstrap confidence values are shown at nodes (1000 replications). Scale bar: amount of inferred evolutionary change along the branch lengths.

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