Parentage determination of cuttlefish (Sepiella japonica) based on microsatellite DNA markers

in Animal Biology
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Abstract

Microsatellite markers have been used for more than ten years to elucidate parentage relationships in aquaculture species. This study aimed to assess the feasibility of utilizing microsatellite markers for parentage determination in cuttlefish (Sepiella japonica) using simulations and real data analysis. We developed a panel of eight microsatellite markers in our lab. These markers were highly polymorphic with a mean of 10.1 alleles and an average expected heterozygosity value of 0.809. Using five simple sequence repeat markers, an allele frequency data-based simulation indicated that the combined exclusion probability values would be over 99%, whereas the rate of assignment success for the real data set was 91.8%. Mismatches caused by null alleles and scoring errors at microsatellite loci were the major reasons for the discrepancies between the simulations and real data analysis. We concluded that microsatellite markers can be used as a powerful tool to evaluate the effectiveness of enhancement and release programs for S. japonica.

Parentage determination of cuttlefish (Sepiella japonica) based on microsatellite DNA markers

in Animal Biology

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Figures

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    Characteristics of the eight polymorphic microsatellite markers of Sepiella japonica.

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    Genotypes of seven female and seven male parents at eight microsatellite DNA markers in Sepiella japonica.

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    Genetic diversity of eight microsatellite markers in 173 Sepiella japonica individuals.

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    Exclusion probabilities of microsatellite markers from the computer simulations.

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    Combined exclusion probabilities of the nine polymorphic loci analyzed for parents unknown (E-1P) and one parent known (E-2P). 1-8 loci with the most PIC one to the least PIC one. Locus S.m21 could not be detected in the genotype of parent Fe#5 and Ma#2, Ma#3, Ma#4, Ma#5 and Ma#7 and Fe#2, Fe#3, Fe#4, Fe#5, Fe#6 and Fe#7 were identified as having contributed to the offspring pool.

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    A total 146 of 159 offspring could be assigned to their parent pairs based on the real data. No offspring was found of two males (Ma#1 and Ma#6) and one female (Fe#1).

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