The bovine sex-determining region Y (Sry) gene and its mRNA transcript are present in Y sperm but not X sperm of bulls
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Abstract
The aims of this study were to establish whether the sex-determining region Y gene and its mRNA transcript are present in the Y sperm and X sperm of bulls and, if present, determine their cellular localization. Semen was collected from three bulls and sorted by flow cytometry into X- and Y-chromosome populations. Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine Sry mRNA expression in X sperm and Y sperm. The presence and localization of Sry DNA and RNA were investigated by fluorescence in situ hybridization (FISH). RT-PCR detected a single Sry transcript of 142 bp in Y sperm but not in X sperm. In Y sperm, the FISH-positive rates for Sry DNA and Sry RNA did not differ significantly from the re-analyzed Y sperm purity. In further experiments, there were no significant differences between the FISH-positive rate for Sry RNA and the re-analyzed Y sperm purity for X-sorted, Y-sorted, or unsorted sperm. In conclusion, FISH analysis revealed that Sry transcripts are present at the edges of the sperm heads of Y sperm but are absent from X sperm.
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The bovine sex-determining region Y (Sry) gene and its mRNA transcript are present in Y sperm but not X sperm of bulls
In: Animal BiologySearch for other papers by Chun-Mei Han in
Current site
Google Scholar
PubMed
Search for other papers by Rong Chen in
Current site
Google Scholar
PubMed
Search for other papers by Tao Li in
Current site
Google Scholar
PubMed
Search for other papers by Xiao-Li Chen in
Current site
Google Scholar
PubMed
Search for other papers by Yong-Fu Zheng in
Current site
Google Scholar
PubMed
Search for other papers by Meng-Ting Ma in
Current site
Google Scholar
PubMed
Search for other papers by Qing-Hua Gao in
Current site
Google Scholar
PubMed
Abstract
The aims of this study were to establish whether the sex-determining region Y gene and its mRNA transcript are present in the Y sperm and X sperm of bulls and, if present, determine their cellular localization. Semen was collected from three bulls and sorted by flow cytometry into X- and Y-chromosome populations. Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine Sry mRNA expression in X sperm and Y sperm. The presence and localization of Sry DNA and RNA were investigated by fluorescence in situ hybridization (FISH). RT-PCR detected a single Sry transcript of 142 bp in Y sperm but not in X sperm. In Y sperm, the FISH-positive rates for Sry DNA and Sry RNA did not differ significantly from the re-analyzed Y sperm purity. In further experiments, there were no significant differences between the FISH-positive rate for Sry RNA and the re-analyzed Y sperm purity for X-sorted, Y-sorted, or unsorted sperm. In conclusion, FISH analysis revealed that Sry transcripts are present at the edges of the sperm heads of Y sperm but are absent from X sperm.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 790 | 81 | 7 |
| Full Text Views | 283 | 3 | 0 |
| PDF Views & Downloads | 83 | 7 | 1 |