Seasonal changes in the protein profile and enzyme activity of the epididymal luminal fluid in the lizard, Eutropis carinata (Schneider, 1801)

in Animal Biology
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Abstract

The epididymis of the male reproductive system is known to be involved in sperm maturation via the production of polypeptides, glycoproteins, surface proteins, enzymes and other factors. During the annual reproductive cycle, the epididymis of the lizard Eutropis carinata undergoes dramatic changes, both morphologically and biochemically, that occur in a well-organized sequence. The present study reveals the sequential changes that occur in the production and concentration of proteins in the epididymal luminal fluid throughout the annual reproductive cycle. A one-dimensional electrophoretic profile of the epididymal luminal proteins revealed a total of 18 bands in the regenerative phase, 22 bands during breeding and 17 bands in the post-breeding as well as regressed phases of the reproductive cycle. By two-dimensional electrophoresis, the protein complexes that are unique to the breeding phase were further resolved based on their pI and the molecular weight of each protein of the protein complex was determined. This is the first study to observe that proteins that are present during the reproductively inactive phase disappear during the reproductively active phase. The Periodic Acid Schiff (PAS) test for protein profiles revealed the presence of proteins with a carbohydrate moiety. Certain enzymes, such as acid phosphatase, alkaline phosphatase, and α-glucosidase, are highly sensitive to seasonal changes and their activity parallels the production of the epididymal proteins. This study provides evidence for androgen-dependent cyclical changes in the pattern of protein profiles and enzyme activity of the epididymal lumen in the lizard E. carinata.

Seasonal changes in the protein profile and enzyme activity of the epididymal luminal fluid in the lizard, Eutropis carinata (Schneider, 1801)

in Animal Biology

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References

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Figures

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    Weight of the epididymis of the lizard, E. carinata during successive phases of the reproductive cycle.

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    Total protein content of the epididymal luminal fluid in the lizard, E. carinata during successive phases of the reproductive cycle.

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    SDS-PAGE profile of epididymal luminal fluid proteins in the lizard, E. carinata during the breeding, regenerative, regressive and post-breeding phases of the reproductive cycle. Abbreviations (as they appear from left to right on the gel): Sm, Standard marker; Br, breeding phase; Reg, regenerative phase; Regr, regressed phase; Pb, post-breeding phase.

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    Master gel image generated by PDQuest software representing protein spots detected upon analysis of 2D gels of protein derived from luminal fluid from the epididymis in the lizard, E. carinata during the breeding phase. The proteins were fractionated in the mass range of 97.4-14.2 kDa and in the pH range 3-11.

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    PAS-stained SDS-PAGE profile of epididymal luminal fluid proteins in the lizard, E. carinata. Abbreviations (as they appear from left to right on the gel): Sm, standard marker; +ve, positive sample (BSA); Br, breeding phase; Pb, post-breeding phase; Regr, regressed phase; Reg, regenerative phase; −ve, negative sample (trypsin).

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    Activities of the enzymes acid phosphatase, alkaline phosphatase and alpha glucosidase in the lumen of the epididymis in the lizard, E. carinata during successive phases of the reproductive cycle.

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