Hox gene expression profiles during embryonic development of common sole

in Animal Biology
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Abstract

Common sole (Solea solea) aquaculture production is based mostly on wild-caught breeders. Recently, the successful reproduction of first-generation fish that were reared in captivity was accomplished. A consistent good quality and quantity of produced eggs throughout the year, and of next-generation broodstock, is important for reducing the overall cost of production. Hox genes play a pivotal role in normal embryonic development and alterations of their temporal expression level may be important for egg viability. Expression profile analysis of five hox genes (hoxa1a, hoxa2a, hoxa2b, hoxb1a and hoxb1b) involved in early embryonic development and of hoxa13a, which is involved in late stages, was carried out. Results revealed a premature and/or maternal expression of hoxa13a in sole embryos, and the detection of hoxa2a and hoxa2b genes as members of paralog group 2. Principal Component Analysis of hox gene expression in 54 ± 6 hours post fertilization embryos coming from wild-caught broodstock and a first-generation one reared in the hatchery, unveiled that these broodstocks are clearly distinct. In addition, their pairwise comparison revealed significant differences in the expression levels of hoxb1a and hoxb1b genes. Hox gene regulation during embryonic development could give valuable insight into rearing sole broodstocks with different origin in concert, and also into gaining a steady mass production of eggs, either in quality or quantity, all year round.

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References

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Figures

  • P broodstock relative hox gene expression (6 ± 6-102 ± 6 hpf). (A) PG1 (hoxa1a, hoxb1a and hoxb1b). (B) PG2 (hoxa2a and hoxa2b). (C) hoxa13a. Dotted line represents the expression level of hox genes at 54 ± 6 hpf. (a) P<0.05, (b) P<0.01, (c) P<0.005, (d) P<0.001.

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  • Heat map showing cluster analysis of observations (samples given on x-axis) according to similarity in hox gene expression during embryonic development (6 ± 6-102 ± 6 hpf). Autoscaled RT-qPCR expression data were used. Each row represents the expression profile of a single gene across development. Three distinct groups were formed. Similarities in hox gene expression were observed between the sampling times of 6 ± 6 and 12 ± 6 hpf, 30 ± 6 and 102 ± 6 hpf, and 54 ± 6 and 78 ± 6 hpf.

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  • PC analysis of hox gene expression level at 54 ± 6 hpf forming two separate groups (P & G1).

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  • Pairwise comparison of hox gene expression in embryo batches per broodstock (P or G1). Symbols: Expression level 66% higher than the mean value (article image), expression level 33% lower than the mean value (article image) and expression level between 33 and 66% of the mean value (article image). Among the candidate reference genes, rps4 presents the minimum variation in expression.

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  • Differential hox gene expression of P and G1 egg batches at 54 ± 6 hpf. RT-qPCR data (Ct values). Mean ± S.E. (a) P<0.05; (d) P<0.001.

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