2 Calle Mar Egeo 7, 11407 Jerez de la Frontera, Cadiz, Spain
3 Departamento de Biología Animal, Biología Vegetal y Ecología, Facultad de Ciencias, Universidad de A Coruña, 15071 A Coruña, Spain;, Email: pgalan@udc.Es
4 Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany, Department of Biological Sciences, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, km 16, 45662-900 Ilhéus,
5 Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany
6 Zoological Institute, Technical University of Braunschweig, Mendelssohnstr. 4, 38106 Braunschweig, Germany, epartment of Comparative Anatomy, Institute of Zoology, Jagiellonian University, ul. Gronostajowa 9, 30-387 Kraków, Poland
Based on DNA sequences of fragments of the mitochondrial COI and 16S rRNA genes we provide a first assessment of genetic variability of Iberian treefrog populations that have been historically allocated to Hyla arborea, partly as subspecies H. a. molleri. Our data from 147 specimens and 33 populations confirm strong divergence between these frogs and Central European H. arborea but relatively low differentiation across their range, supporting their status as a separate species, H. molleri. Preliminary phylogeographic data indicate a possible weak genetic differentiation of populations from the northern coast of the region of Galicia. We suggest inclusion of nuclear markers and an extension of the sampling into the coastal regions of Asturias and Cantabria, as well as the identification of the contact zone between H. molleri and H. arborea in either the Spanish Basque country or in France, as priorities for future research on this species.