Weak genetic divergence between the two South American toad-headed turtles Mesoclemmys dahli and M. zuliae (Testudines: Pleurodira: Chelidae)

in Amphibia-Reptilia
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Mesoclemmys dahli and M. zuliae are two endangered, little-known toad-headed turtles with small distribution ranges in Colombia and Venezuela, respectively. Using the mitochondrial cytochrome b gene as a marker, we investigate their phylogeographic differentiation. Furthermore, based on 2341 bp of mtDNA and 2109 bp of nDNA of M. dahli, M. zuliae and allied chelid turtles, we infer their divergence time using a fossil-calibrated relaxed molecular clock approach. Mesoclemmys dahli and M. zuliae are closely related species, with an estimated mean divergence time of 10.6 million years. This estimate correlates with the uplift of the Serranía de Perijá, an Andean mountain chain separating their distribution ranges, suggesting that this event could have caused the evolution of the two species. Haplotype and nucleotide diversities of M. dahli are markedly higher than in Podocnemis lewyana, another endemic turtle species of Colombia. This pronounced dissimilarity may reflect differences in the phylogeographies and demographic histories of the two species, but also different habitat preferences.

Weak genetic divergence between the two South American toad-headed turtles Mesoclemmys dahli and M. zuliae (Testudines: Pleurodira: Chelidae)

in Amphibia-Reptilia

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References

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Figures

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    Distribution of Mesoclemmys dahli and M. zuliae (based on Rueda-Almonacid et al., 2007) and sampling sites (colour-coded). For M. dahli, the Córdoba population is indicated in green and the Cesar population in orange; sites of M. zuliae, blue. The Serranía de Perijá is highlighted in red. Inset: juvenile Mesoclemmys dahli from the Cesar population (photo: Guido Medina).

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    Parsimony network of cyt b haplotypes of Mesoclemmys dahli and M. zuliae, based on an alignment of 1067 bp length. Circle size indicates haplotype frequency. Missing haplotypes are shown as small black circles. Each line connecting haplotypes corresponds to one mutational step. Colour code as in fig. 1; haplotype codes refer to the Appendix.

  • View in gallery

    Bayesian tree based on 2341 bp of mitochondrial DNA (12 S rRNA, ND4, cyt b) and 2109 bp of nuclear DNA sequences (C-mos, R35, Rag 2; partitioned by gene). Support values are Bayesian posterior probabilities (top) and ML bootstrap values (bottom); asterisks indicate maximum support under both methods. This tree was used for the Relaxed Molecular Clock calculations.

  • View in gallery

    Divergence time estimates using the MULTIDISTRIBUTE package. Dark grey bars at nodes represent 95% confidence intervals. The light grey column on the right shows the time of the main uplift of the Serranía de Perijá (Late Miocene-Pliocene; Kellogg, 1984). Letters indicate nodes calibrated with fossil evidence (see table 1). PP, Plio-Pleistocene.

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