Nuclear and mitochondrial markers reveal the existence of several geographically concordant lineages within a Sahelian gecko species, Ptyodactylus ragazzii

in Amphibia-Reptilia
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The genetic diversity within Ptyodactylus ragazzii was analysed for the first time across the Western part of its range. We have used two mitochondrial (12s rRNA and 16s rRNA) and one nuclear (Cmos) marker to compare results directly with other related Ptyodactylus species, P. oudrii and P. hasselquistii. Results show high levels of intraspecific variability, with at least three divergent mtDNA lineages that have different haplotypes for Cmos and that are geographically concordant. P. ragazzii from Mauritania is probably a distinct species and possibly other lineages too, such as those from the Aïr Mountains in Niger, although more nuclear markers are needed to confirm this. All analysed Ptyodactylus species appear to be cryptic species complexes containing multiple deeply divergent forms, highlighting the need for a careful reassessment of the taxonomy of the whole genus.

Nuclear and mitochondrial markers reveal the existence of several geographically concordant lineages within a Sahelian gecko species, Ptyodactylus ragazzii

in Amphibia-Reptilia

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Figures

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    Study area and location of newly sequenced samples of P. ragazzii. Sample codes are indicated. Circles, triangles, and squares in the large map represent phylogenetic haplogroups obtained in the tree (fig. 2). Small map represents the distribution of the other species of Ptyodactylus included (modified from Sindaco and Jeremcenko, 2008 to include the recently identified Mauritanian population). White circles: P. oudrii; dark gray circles: P. guttatus; white squares: P. hasselquistii; dark gray squares: P. ragazzii.

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    Left: Phylogenetic tree obtained by BI and ML analysis of the combined mtDNA fragments (12s and 16s). Support values (%) are given as Bayesian posterior probability/Bootstrap support. Dark circles indicate 100/100 support. Some terminal node values were omitted for clarity. Right: BEAST maximum clade credibility tree for the sampled Ptyodactylus lineages. Divergence times correspond to the mean posterior estimate of their age in million years. The grey bars indicate the height 95% HPD interval. Nodes with a posterior probability < 0.95 do not present a bar.

  • View in gallery

    Haplotype (median joining) network showing the relationships of Ptyodactylus species, highlighted for P. oudrii and P. ragazzii, inferred from 339 bp Cmos nuclear sequences. Circle sizes are proportional to the number of samples sharing the same haplotype. Dashes represent mutated positions. Black circles represent median vectors.

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