Incongruence between taxonomy and genetics: three divergent lineages within two subspecies of the rare Transcaucasian rat snake (Zamenis hohenackeri)

in Amphibia-Reptilia
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Two morphologically differentiated subspecies with presumably allopatric distribution have traditionally been recognized in the rare Transcaucasian rat snake, Zamenis hohenackeri: Z. h. hohenackeri (Caucasus region and E Turkey, NW Iran and N Iraq) and Z. h. tauricus (S Anatolia and Levant). Both subspecies are sometimes considered just colour forms of a monotypic species. We used sequences of the mitochondrial gene cytochrome b to find out whether both subspecies are also genetically differentiated, or whether their haplotypes form a cluster without clear subdivision. We found that the species forms three divergent lineages: one corresponds to the nominotypic subspecies, another comprises an individual from the region of the type locality of Z. h. tauricus in southern Anatolia and the third, with no available name and separated from the previous by the Amanos (Nur) Mountains, is distributed in the Levant. The pattern of divergence and distribution of the lineages correspond to other species of amphibians and reptiles, demonstrating that similar geo-climatic events presumably formed genetic variation and triggered speciation processes in this region.

Incongruence between taxonomy and genetics: three divergent lineages within two subspecies of the rare Transcaucasian rat snake (Zamenis hohenackeri)

in Amphibia-Reptilia

References

AfrasiabS.R.MohamadS.I. (2011): First record of the rat snake, Zamenis hohenackeri (Strauch, 1873), from north-eastern Iraq with notes on other colubrid species (Reptilia: Colubridae). Zool. Middle East 54: 19-22.

BilginR. (2011): Back to the suture: the distribution of intraspecific genetic diversity in and around Anatolia. Int. J. Mol. Sci. 12: 4080-4103.

BischoffW. (1993): Elaphe hohenackeri (Strauch, 1873), Transkaukasische Kletternatter. In: Handbuch der Reptilien und Amphibien Europas Band 3/I. Schlangen (Serpentes) I p.  317-329. BöhmeW. Ed. Aula-VerlagWiesbaden.

BurbrinkF.T.LawsonR. (2007): How and when did Old World rat snakes disperse into the New World? Mol. Phylogenet. Evol. 43: 173-189.

BurbrinkF.T.LawsonR.SlowinskiJ.B. (2000): MtDNA phylogeography of the North American rat snake (Elaphe obsoleta): a critique of the subspecies concept. Evolution 54: 2107-2118.

DavisP.H. (1971): Distribution patterns in Anatolia with particular reference to endemism. In: Plant Life of South-West Asia p.  15-27. DavisP.H.HarperP.C.HedgeI.C. Eds Botanical Society of EdinburghEdinburgh.

GuindonS.DufayardJ.-F.LefortV.AnisimovaM.HordijkW.GascuelO. (2010): New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59: 307-321.

GvoždíkV.MoravecJ.KlütschK.KotlíkP. (2010): Phylogeography of the Middle Eastern tree frogs (Hyla, Hylidae, Amphibia) as inferred from nuclear and mitochondrial DNA variation, with a description of a new species. Mol. Phylogenet. Evol. 55: 1146-1166.

GvoždíkV.JandzikD.CordosB.RehákI.KotlíkP. (2012): A mitochondrial DNA phylogeny of the endangered vipers of the Vipera ursinii complex. Mol. Phylogenet. Evol. 62: 1019-1024.

HrbekT.StöltingK.N.BardakciF.KüçükF.WildekampR.H.MeyerA. (2004): Plate tectonics and biogeographical patterns of the Pseudophoxinus (Pisces: Cypriniformes) species complex of central Anatolia, Turkey. Mol. Phylogenet. Evol. 23: 297-308.

HuelsenbeckJ.P.RonquistF. (2001): MRBAYES: Bayesian inference of phylogeny. Bioinformatics 17: 754-755.

JohnsG.C.AviseJ.C. (1998): A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Mol. Biol. Evol. 15: 1481-1490.

KapliP.BotoniD.IlgazC.KumlutaşY.AvcıA.Rastegar-PouyaniN.FathiniaB.LymberakisP.AhmadzedehF.PolakakisN. (2013): Molecular phylogeny and historical biogeography of the Anatolian lizard Apathya (Squamata, Laceridae). Mol. Phylogenet. Evol. 66: 992-1001.

LibradoP.RozasJ. (2009): DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451-1452.

LitvinchukS.N.ZuiderwijkA.BorkinL.J.RosanovJ.M. (2005): Taxonomic status of Triturus vittatus (Amphibia: Salamandridae) in western Turkey: trunk vertebrae count, genome size and allozyme data. Amph.-Rept. 26: 305-323.

MusilováR.ZavadilV.MarkováS.KotlíkP. (2011): Relics of the Europe’s warm past: Phylogeography of the Aesculapian snake. Mol. Phylogenet. Evol. 57: 1245-1252.

NagyZ.T.LawsonR.JogerU.WinkM. (2004): Molecular systematics of racers, whipsnakes and relatives (Reptilia: Colubridae) using molecular and nuclear markers. J. Zool. Syst. Evol. Res. 42: 223-233.

NilsonG.AndrénC. (1984): A taxonomic account of the Iranian ratsnakes of the Elaphe longissima species-group. Amph.-Rept. 5: 157-171.

NylanderJ.A.A. (2004): MrModeltest v2. Program Distributed by the Author. Evolutionary Biology Centre, Uppsala UniversityUppsala.

PosadaD. (2008): JModelTest: phylogenetic model averaging. Mol. Phylogenet. Evol. 25: 1253-1256.

PyronR.A.BurbrinkF.T.ColliG.R.Montes de OcaA.N.VittL.J.KuczynskiC.A.WiensJ.J. (2011): The phylogeny of advanced snakes (Colubroidea), with discovery of a new subfamily and comparison of support methods for likelihood trees. Mol. Phylogenet. Evol. 58: 329-342.

RonquistF.HuelsenbeckJ.P. (2003): MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.

SchulzK.-D. (1996): Eine Monographie der Schlangengattung Elaphe Fitzinger. Bushmaster Publications.

SindacoR.VenchiA.CarpanetoG.M.BolognaM.A. (2000): The reptiles of Anatolia: a checklist and zoogeographical analysis. Biogeographia 21: 441-554.

SindacoR.VenchiA.GriecoC. (2013): The Reptiles of the Western Palearctic Volume 2 Annotated Checklist and Distributional Atlas of the Snakes of Europe North Africa Middle East and Central Asia with an Update to Volume 1. Edizioni Belvedere.

SlowinskiJ.B.WüsterW. (2000): A new cobra (Elapidae: Naja) from Myanmar (Burma). Herpetologica 56: 257-270.

StrauchA. (1873): Die Schlangen des Russischen Reichs in systematischer und zoogeographischer Beziehung. Mém. Acad. Sci Pétersbourg Ser. 7 21: 1-288.

StümpelN.JogerU. (2009): Recent advances in phylogeny and taxonomy of Near and Middle Eastern vipers – an update. ZooKeys 31: 179-191.

SwoffordD.L. (2003): PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4. Sinauer AssociatesSunderland, USA.

VenchiA.SindacoR. (2006): Annotated checklist of the reptiles of the Mediterranean countries, with keys to species identification. Part 2 – Snakes (Reptilia, Serpentes). Ann. Mus. Civ. Stor. Nat. “G. Doria” 98: 259-364.

WernerF. (1898): Über einige neue Reptilien und einen neuen Frosch aus dem cilicischen Taurus. Zool. Anz. 21: 217-223.

WüsterW.DumbrellA.J.HayC.PookC.E.WilliamsA.J.FryB.G. (2005): Snakes across the Strait: trans-Torresian phylogeographic relationships in three genera of Australasian snakes (Serpentes: Elapidae: Acanthophis, Oxyuranus, and Pseudechis). Mol. Phylogenet. Evol. 34: 1-14.

ZamudioK.R.GreeneH.W. (1997): Phylogeography of the bushmaster (Lachesis muta: Viperidae): implications for neotropical biogeography, systematics, and conservation. Biol. J. Linn. Soc. 62: 421-442.

Figures

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    Map showing the origin of Z. hohenackeri samples used in this study. The range of Z. hohenackeri in dark grey colour is based on published records reviewed in Schulz (1996) and Sindaco, Venchi and Grieco (2013). It is not clear whether the records from northern central Turkey and western Iran (dark grey circles) represent isolated occurrences or extremes of a continuous distribution. Origin of the samples: Z. h. hohenackeri – 1) Chechenia, Russia, pet trade sample, 2) Boshuri, Georgia, 3) Artvin, Turkey, 4) Trabzon, Turkey (Burbrink and Lawson, 2007); Z. h. tauricus – 5) Adana, Turkey; Z. hohenackeri Southern lineage – 6) Hatay, Turkey, 7) Bcharré (Cedars), Lebanon.

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    Maximum likelihood haplotype tree of 1102 bp fragment of Cytb mtDNA showing the phylogenetic relationships within the genus Zamenis, with emphasis on Z. hohenackeri. The substitution model TIM2 + G was selected under AIC. The statistical support of the branches is expressed as percentage bootstrap values/Bayesian posterior probabilities. Letters/numbers accompanying the terminal clade names are haplotype names; (GB) – sequences obtained from GenBank.

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