Distribution and hybridization of Anguis fragilis and A. colchica in Hungary

in Amphibia-Reptilia
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Slow worms (Anguis spp.) are widely distributed in Europe. Based on pronounced divergences in molecular markers the subspecies of the slow worm, Anguis fragilis, have been recently elevated to species level. In Hungary both A. fragilis and A. colchica are present in the mountainous areas with their range being separated by the Danube River with potential contact zones in the Danube valley. Based on morphology, hybridization of the two taxa has been described earlier from the Budai and Pilis Mountains. In order to reveal the exact distribution and confirm hybridization of Anguis taxa in Hungary we analyzed fragments of mitochondrial (ND2) and nuclear (Rag1) genes in 36 specimens from eight regions of Hungary and adjacent countries. The results confirmed the previously known distribution pattern with an east-west split along the Danube River and supported the morphological findings about hybridization in the Budai and Pilis Mountains.

Distribution and hybridization of Anguis fragilis and A. colchica in Hungary

in Amphibia-Reptilia

References

CabelaA.GrillitschH. (1989): Zum systematischen Status der Blindschleiche (Anguis fragilis Linnaeus, 1758) von Nordgriechenland und Albanien (Squamata: Anguidae). Herpetozoa 2: 51-69.

ChennaR.SugawaraH.KoikeT.LopezR.GibsonT.J.HigginsD.G.ThompsonJ.D. (2003): Multiple sequence alignment with the Clustal series of programs. Nucl. Acids Res. 31: 3497-3500.

DelyO.Gy. (1972): Beiträge zur Verbreitung und Systematik der Blindschleiche (Anguis fragilis Linnaeus) in Karpatenbecken. Vertebr. Hung. 13: 39-80.

DelyO.Gy. (1974a): Über die Unterarten der Blindschleiche, Anguis fragilis L. Vertebr. Hung. 15: 11-37.

DelyO.Gy. (1974b): Systematische und Verbreitungsprobleme der Blindschleiche (Anguis fragilis Linnaeus). Állattani Közl. 61: 17-26.

DelyO.Gy. (1981): Anguis fragilis Linnaeus 1758 – Blindschleiche. In: Handbuch der Reptilien und Amphibien Europas. Band 1. Echsen (Sauria) 1 p.  241-258. BöhmeW. Ed. AULA-VerlagWiesbaden.

FritzU.GuickingD.KamiH.ArakelyanM.AuerM.AyazD.Ayres FernándezC.BakievA.G.CelaniA.DžukićG.FahdS.HavašP.JogerU.KhabibullinV.F.MazanaevaL.F.ŠirokýP.TripepiS.Valdeón VélezA.Velo AntónG.WinkM. (2007): Mitochondrial phylogeography of European pond turtles (Emys orbicularis, Emys trinacris) – an update. Amphibia-Reptilia 28: 418-426.

GrillitschH.CabelaA. (1990): Zum systematischen Status der Blindschleichen (Squamata: Anguidae) der Peloponnes und der südlichen Ionischen Inseln (Griechenland). Herpetozoa 2: 131-153.

GrothJ.G.BarrowcloughG.F. (1999): Basal divergences in birds and the phylogenetic utility of the nuclear RAG-1 gene. Mol. Phylogenet. Evol. 12: 115-123.

GuindonS.GascuelO. (2003): A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol. 52: 696-704.

GvoždíkV.JandzikD.LymberakisP.JablonskiD.MoravecJ. (2010): Slow worm, Anguis fragilis (Reptilia: Anguidae) as a species complex: Genetic structure reveals deep divergences. Mol. Phylogenet. Evol. 55: 460-472.

GvoždíkV.BenkovskýN.CrottiniA.BellatiA.MoravecJ.RomanoA.SacchiR.JandzikD. (2013): An ancient lineage of slow worms, genus Anguis (Squamata: Anguidae), survived in the Italian Peninsula. Mol. Phylogenet. Evol. doi:10.1016/j.ympev.2013.05.004

HordijkW.GascuelO. (2005): Improving the efficiency of SPR moves in phylogenetic tree search methods based on maximum likelihood. Bioinformatics 21: 4338-4347.

KindlerC.BöhmeW.CortiC.GvoždíkV.JablonskiD.JandzikD.MetallinouM.ŠirokýP.FritzU. (2013): Mitochondrial phylogeography, contact zones and taxonomy of grass snakes (Natrix natrix, N. megalocephala). Zoologica Scripta doi:10.1111/zsc.12018

LácJ. (1967): To the systematics of the slow worm (Anguis fragilis L.) and its distribution in Slovakia. Biologia 22: 908-921.

MayerW.GrillitschH.CabelaA. (1991): Proteinelektrophoretische Untersuchungen zur Systematik der südgriechischen Blindschleiche (Squamata: Anguidae). Herpetozoa 4: 157-165.

MoravecJ. (1997): Anguis fragilis Linnaeus, 1758. In: Czech Recent and Fossil Amphibians and Reptiles. An Atlas and Field Guide p.  78-79. NečasP.ModrýD.ZavadilV. Eds Edition ChimairaFrankfurt am Main.

MustersC.J.M.in den BoschH.J.A. (1982): Einige Bemerkungen zu den Unterarten von Anguis fragilis L., mit Berücksichtigung niederländischer Exemplare (Reptilia: Sauria: Anguidae). Salamandra 18: 196-204.

PetzoldH.G. (1971): Blindschleiche und Scheltopusik. Die Familie Anguidae. Die Neue Brehm-Bücherei 448. A. ZiemsenWittenberg-Lutherstadt.

PosadaD. (2008): jModelTest: phylogenetic model averaging. Mol. Biol. Evol. 25: 1253-1256.

PosadaD. (2011): Collapse: Describing haplotypes from sequence alignments. [Online]. Website last modified on May 28 2011 (accessed on August 11 2011). Available at http://darwin.uvigo.es/software/collapse.html

RonquistF.HuelsenbeckJ.P. (2003): MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.

RozasJ. (2009): DNA sequence polymorphism analysis using DnaSP. In: Bioinformatics for DNA Sequence AnalysisMethods in Molecular Biology SeriesVol. 537 p.  337-350. PosadaD. Ed. Humana PressNJ, USA.

SchmittT. (2007): Molecular biogeography of Europe: Pleistocene cycles and postglacial trends. Front. in Zool. 4: 11.

StojanovA. (2001): Beobachtung einer Kopulation zwischen den beiden Unterarten der Blindschleiche (Anguis fragilis L.). Hist. Nat. Bulgarica 13: 155-157.

VölklW.AlfermannD. (2007): Die Blindschleiche–die vergessene Echse. Z. Feldherpetologie Beiheft 11. Laurenti-VerlagBielefeld.

VörösJ.AlcobendasM.Martínez-SolanoI.García-ParísM. (2006): Evolution of Bombina bombina and Bombina variegata (Anura: Discoglossidae) in the Carpathian Basin: a history of repeated mt-DNA introgression across species. Mol. Phylogen. Evol. 38: 705-718.

Figures

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    Distribution of Anguis species in Central Europe, sampling sites, ND2 Bayesian phylogram and Rag1 genotype assignment of Hungarian Anguis samples. The sampling location in Spain is not shown on the map. On the ND2 phylogram haplotypes found in samples of the present study are indicated as AF = A. fragilis, AC = A. colchica. Outgroup species (A. cephallonica and A. graeca) and other A. fragilis/colchica haplotypes (c1-c10, f1-f6) are retrieved from NCBI (Gvoždík et al., 2010). Bayesian posterior probabilities/ML bootstrap support values are given at the nodes. Shaded positions represent Rag1 diplotypes referring to hybrid origin. This figure is published in colour in the online version.

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