Phylogeography and postglacial colonization of Central Europe by Anguis fragilis and Anguis colchica

in Amphibia-Reptilia
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Five slow-worm species are distributed in the Palearctic region. Two species, Anguis fragilis and A. colchica, are native to Central Europe, where only limited information on the phylogeography of the genus exists. Here, we examined the situation in Poland and surrounding regions, where a mitochondrial contact zone between the species is expected. We used new mitochondrial DNA sequences and available published data from Central Europe and the northern Balkans. Haplotypes of both species were recorded in the study area. Anguis fragilis is represented by a single haplogroup, while A. colchica by three haplogroups. This suggests four independent sources/refugia for postglacial colonization of northern Central Europe. The mitochondrial contact zone seems to mirror the borders between lowlands of the North European Plain and East European Plain, and the south-eastern Poland uplands, while the Vistula River does not constitute a barrier. The presence of both species, A. fragilis and A. colchica, in Poland should also be considered for protection by the Polish conservation legislation.

Amphibia-Reptilia

Publication of the Societas Europaea Herpetologica

References

ArnoldE.N. (2002): Reptiles and Amphibians of Europe. Princeton Univeristy Press, Princeton and Oxford.

BjörckS. (1995): A review of the history of the Baltic Sea, 13.0-8.0 ka BP. Quatern. Int. 27: 19-40.

ClementM.PosadaD.CrandallK.A. (2000): TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9: 1657-1659.

DelyO.G. (1972): Beiträge zur Verbreitung und Systematik der Blindschleiche (Anguis fragilis Linnaeus) im Karpatenbecken. Vertebr. Hungarica 13: 39-79.

dos SantosA.M.CabezasM.P.TavaresA.I.XavierR.BrancoM. (2016): tcsBU: a tool to extend TCS network layout and visualization. Bioinformatics 32: 627-628.

DufresnesC.WassefJ.GhaliK.BrelsfordA.StöckM.LymberakisP.Crnobrnja-IsailovicJ.PerrinN. (2013): Conservation phylogeography: does historical diversity contribute to regional vulnerability in European tree frogs (Hyla arborea)? Mol. Ecol. 22: 5669-5684.

DufresnesC.LitvinchukS.N.LeuenbergerJ.GhaliK.ZinenkoO.StöckM.PerrinN. (2016): Evolutionary melting pots: a biodiversity hotspot shaped by ring diversification around Black Sea in the eastern tree frog (Hyla orientalis). Mol. Ecol. 25: 4285-4300.

GłowacińskiZ.RafińskiJ. (2003): Atlas of Amphibians and Reptiles in Poland (in Polish: Atlas płazów i gadów Polski). Biblioteka Monitoringu Środowiska, Warszawa/Kraków.

GvoždíkV.JandzikD.LymberakisP.JablonskiD.MoravecJ. (2010): Slow worm, Anguis fragilis (Reptilia: Anguidae) as a species complex: genetic structure reveals deep divergences. Mol. Phylogenet. Evol. 55: 460-472.

GvoždíkV.BenkovskýN.CrottiniA.BellatiA.MoravecJ.RomanoA.SacchiR.JandzikD. (2013): An ancient lineage of slow worms, genus Anguis (Squamata: Anguidae), survived in the Italian Peninsula. Mol. Phylogenet. Evol. 69: 1077-1092.

GvoždíkV.MoravecJ.ZavadilV.JeřábkováL. (2015a): Anguis fragilis and A. colchica – distribution in the Czech Republic (in Czech: Slepýš křehký a slepýš východní – výskyt v České republice). In: Fauna ČR. Plazi – Reptilia, p.  275-278. MoravecJ., Ed., Academia, Praha.

GvoždíkV.CanestrelliD.García-ParísM.MoravecJ.NascettiG.RecueroE.TeixeiraJ.KotlíkP. (2015b): Speciation history and widespread introgression in the European short-call tree frogs (Hyla arborea sensu lato, H. intermedia and H. sarda). Mol. Phylogenet. Evol. 83: 143-155.

JablonskiD.JandzikD.MikulíčekP.DžukićG.LjubisavljevićK.TzankovN.JelićD.ThanouE.MoravecJ.GvoždíkV. (2016): Contrasting evolutionary histories of the legless lizards slow worms (Anguis) shaped by the topography of the Balkan Peninsula. BMC Evol. Biol. 16: 99. DOI:10.1186/s12862-016-0669-1.

JuszczykW. (1987): Polish Amphibians and Retiles. Part 3: Reptiles (in Polish: Płazy i gady krajowe. Czȩść 3: Gady). PWN, Warszawa.

KindlerC.BöhmeW.CortiC.GvoždíkV.JablonskiD.JandzikD.MetallinouM.ŠirokýP.FritzU. (2013): Mitochondrial phylogeography, contact zones and taxonomy of grass snakes (Natrix natrix, N. megalocephala). Zool. Scripta 42: 458-472.

KindlerC.BringsøeH.FritzU. (2014): Phylogeography of grass snakes (Natrix natrix) all around the Baltic Sea: implications for the Holocene colonization of Fennoscandia. Amphibia-Reptilia 35: 413-424.

LibradoP.RozasJ. (2009): DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25: 1451-1452.

ReutherA.U.UrdeaP.GeigerC.Ivy-OchsS.NillerH.P.KubikP.W.HeineK. (2007): Late Pleistocene glacial chronology of the Pietrele Valley, Retezat Mountains, Southern Carpathians constrained by 10Be exposure ages and pedological investigations. Quat. Int. 164-165: 151-169.

SkórzewskiG. (2017): Remarks on the studies and occurrence of the eastern slow worm Anguis colchica incerta in Poland (in Polish: Uwagi na temat badań i wystȩpowania padalca kolchidzkiego Anguis colchica incerta w Polsce). Chrońmy Przyr. Ojczysta̧ 73: 57-63.

SkórzewskiG.BorczykB.NajbarB. (2012): Morphological differentiation of Anguidae Gray, 1825 in Poland: one or two species? (in Polish: Zróżnicowanie morfologiczne padalcowatych Anguidae Gray. 1825 w Polsce: jeden czy dwa gatunki?). In: Biologia Płazów I Gadów – Ochrona Herpetofauny, p.  117-120. ZamachowskiW., Ed., Wydawnictwo Naukowe Uniwersytetu Pedagogicznego w Krakowie, Kraków.

SommerR.S.LindqvistC.PerssonA.BringsøeH.RhodinA.G.J.SchneeweißN.ŠirokýP.BachmannL.FritzU. (2009): Unexpected early extinction of the European pond turtle (Emys orbicularis) in Sweden and climatic impact on its Holocene range. Mol. Ecol. 18: 1252-1262.

StöckM.DufresnesC.LitvinchukS.N.LymberakisP.BiollayS.BerroneauM.BorzéeA.GhaliK.OgielskaM.PerrinN. (2012): Cryptic diversity among Western Palearctic tree frogs: postglacial range expansion, range limits, and secondary contacts of three European tree frog lineages (Hyla arborea group). Mol. Phylogenet. Evol. 65: 1-9.

SzabóK.VörösJ. (2014): Distribution and hybridization of Anguis fragilis and A. colchica in Hungary. Amphibia-Reptilia 35: 135-140.

ThanouE.GiokasS.KorniliosP. (2014): Phylogeography and genetic structure of the slow worms Anguis cephallonica and Anguis graeca (Squamata: Anguidae) from the southern Balkan Peninsula. Amphibia-Reptilia 35: 263-269.

Figures

  • Distribution of mitochondrial haplogroups and haplotype networks of Anguis fragilis and A. colchica in Central Europe based on new and published data (Gvoždík et al., 2010, 2013; Szabó and Vörös, 2014; Jablonski et al., 2016; terminology as well as colours of haplogroups follows the latter publication). The dashed orange line denotes approximate location of the mitochondrial contact zone between the species. Whitish transparent shading shows the distribution range of the genus. Question marks denote missing phylogeographic data. Small black dots in the haplotype networks represent unsampled or extinct haplotypes. Bold lines of haplotypes in the networks highlight haplotypes found in the present study (including outside Poland). For clear association of the found haplotypes to the previously published haplotypes (based on a longer mtDNA fragment), we list GenBank numbers and the original haplotype name: f1 – f3, f7, f12, f13 (A. fragilis) and c1 – c6, c12 (A. colchica); Gvoždík et al. (2010, 2013). “S” in the inset map indicates specimens identified based on the morphology (Skórzewski, 2017).

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