Invasive mosquitofish (Gambusia holbrooki) affect egg-laying and behaviour of Spanish pygmy newts (Triturus pygmaeus)

in Amphibia-Reptilia
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Invasive species are one of the main causes of amphibian declines worldwide, often through direct predation. Even species or life stages that may not be prone to predation by invasive animals can be affected through alterations of their reproductive behaviour and/or performance. This aspect is less commonly investigated, and may be important for understanding the full impact of invasive species on local amphibian populations. We used laboratory experiments to measure effects of the invasive Eastern mosquitofish (Gambusia holbrooki) on gravid pygmy newts (Triturus pygmaeus) from southern Spain. Gravid newts altered their position in the water column by moving from the bottom of the aquaria to the surface when in the presence of free-swimming mosquitofish, presumably to reduce physical contact with them. Newts also detected and consumed less prey in presence of free mosquitofish. Newts exposed to caged or free-swimming mosquitofish laid fewer eggs than newts not exposed to the invasive species, suggesting that chemical or visual cues alone were sufficient to alter the behaviour of gravid newts. Our results suggest that mosquitofish can reduce the reproductive success of native pygmy newts in the wild, highlighting the need for management efforts to mitigate this impact.

Invasive mosquitofish (Gambusia holbrooki) affect egg-laying and behaviour of Spanish pygmy newts (Triturus pygmaeus)

in Amphibia-Reptilia



  • AlemadiS.D.JenkinsD.G. (2008): Behavioral constraints for the spread of the eastern mosquitofish, Gambusia holbrooki (Poeciliidae). Biol. Invasions. 10: 59-66.

  • BakerJ.M.R. (1992): Egg-production in the smooth newt (Triturus vulgaris). Herpetol. J. 2: 90-93.

  • BernardesM.RodderD.NguyenT.T.PhamC.T.NguyenT.Q.ZieglerT. (2013): Habitat characterization and potential distribution of Tylototriton vietnamensis in northern Vietnam. J. Nat. Hist. 47: 1161-1175.

  • BlancoS.RomoS.VillenaM.J. (2004): Experimental study on the diet of Mosquitofish (Gambusia holbrooki) under different ecological conditions in a shallow lake. Int. Rev. Hydrobiol. 89: 250-262.

  • BrönmarkC.EdenhamnP. (1994): Does the presence of fish affect the distribution of tree frogs (Hyla arborea)? Conserv. Biol. 8: 841-845.

  • Cabrera-GuzmánE.Díaz-PaniaguaC.Gomez-MestreI. (2017): Competitive and predatory interactions between invasive mosquitofish and native larval newts. Biol. Invasions. 19: 1449-1460.

  • SostoaA. (2005): Possible reasons for the decline of two native toothcarps in the Iberian Peninsula: evidence of competition with the introduced eastern mosquitofish. J. Appl. Ichthyol. 21: 358-363.

  • Cicort-LucaciuA.Ş.DimanceaN.Blaga-LungulescuR.M.HodişanO.BenkőA. (2009): Diet composition of a Triturus dobrogicus (Amphibia) population from Arad County, western Romania. Biharean Biol. 3: 77-82.

  • DavidsonC.KnappR.A. (2007): Multiple stressors and amphibian declines: dual impacts of pesticides and fish on yellow-legged frogs. Ecol. Appl. 17: 587-597.

  • DeSantisD.L.DavisD.R.GaborC.R. (2013): Chemically mediated predator avoidance in the Barton Springs salamander (Eurycea sosorum). Herpetologica. 69: 291-297.

  • Díaz-PaniaguaC. (1989): Oviposition behavior of Triturus marmoratus pygmaeus. J. Herpetol. 23: 159-163.

  • Díaz-PaniaguaC. (1990): Temporary ponds as breeding sites of amphibians at a locality in southwestern Spain. Herpetol. J. 1: 447-453.

  • Díaz-PaniaguaC. (1998): Reproductive dynamics of a population of small marbled newts (Triturus marmoratus pygmaeus) in South-Western Spain. Herpetol. J. 8: 93-98.

  • Díaz-PaniaguaC.Gómez-Rodrí VriesW. (2005): Los anfibios de Doñana. Publicaciones del Organismo Autónomo de Parques Nacionales.

  • Díaz-PaniaguaC.Fernández-ZamudioR.FlorencioM.García-MurilloP.Gómez-RodríguezC.SiljeströmP.SerranoL. (2010): Temporary ponds from the Doñana National Park: a system of natural habitats for the preservation of aquatic flora and fauna. Limnetica 29: 41-58.

  • Díaz-PaniaguaC.Fernández-ZamudioR.SerranoL.FlorencioM.SousaA.García-MurilloP.Siljeström P. (2016): El sistema de lagunas temporales del Parque Nacional de Doñana: aplicación a la gestión y conservación de hábitats acuáticos singulares. Proy. Investig. Parques Nacionales 2011-2014: 37-59.

  • ElviraB.AlmodóvarA. (2001): Freshwater fish introductions in Spain: facts and figures at the beginning of the 21st century. J. Fish Biol. 59: 323-331.

  • FasolaM.CanovaL. (1992): Feeding habits of Triturus vulgaris, T. cristatus and T. alpestris (Amphibia, Urodela) in the northern Apennines (Italy). J. Boll. Zool. 59: 273-280. DOI:10.1080/11250009209386682.

  • GkenasC.OikconomouA.EconomouA.KiosseF.LeonardosI. (2012): Life history pattern and feeding habits of the invasive mosquitofish, Gambusia holbrooki, in Lake Pamvotis (NW Greece). J. Biol. Res. 17: 121-136.

  • Gómez-RodríguezC.Díaz-PaniaguaC.SerranoL.FlorencioM.PortheaultA. (2009): Mediterranean temporary ponds as amphibian breeding habitats: the importance of preserving pond networks. Aquat. Ecol. 43: 1179-1191.

  • GustafsonD.H.AndersenA.S.L.MikusinskiG.MalmgrenJ.C. (2009): Pond quality determinants of occurrence patterns of Great Crested Newts (Triturus cristatus). J. Herpetol. 43: 300-310.

  • HallidayT.R. (1975): On the biological significance of certain morphological characters in males of the Smooth newt Triturus vulgaris and of the Palmate newt Triturus helveticus (Urodela: Salamandridae). Zool. J. Linn Soc. 56: 291-300.

  • HartelT.NemesS.CogălniceanuD.ÖllererK.SchweigerO.MogaC.-I.DemeterL. (2007): The effect of fish and aquatic habitat complexity on amphibians. Hydrobiologia. 583: 173-182.

  • HunterD.A.SmithM.J.ScroggieM.P.GilliganD. (2011): Experimental examination of the potential for three introduced fish species to prey on tadpoles of the endangered Booroolong frog, Litoria booroolongensis. J. Herpetol. 45: 181-185.

  • Ibáñez-ÁlamoJ.D.SolerM. (2012): Predator-induced female behavior in the absence of male incubation feeding: an experimental study. Behav. Ecol. Sociobiol. 66: 1067-1073.

  • IvantsoffW.Aarn (1999): Detection of predation on Australian native fishes by Gambusia holbrooki. Mar. Freshwater Res. 50: 467-468.

  • KatsL.B.FerrerR.P. (2003): Alien predators and amphibian declines: review of two decades of science and the transition to conservation. Divers. Distrib. 9: 99-110.

  • KatsL.B.SihA. (1992): Oviposition site selection and avoidance of fish by streamside salamanders (Ambystoma barbouri). Copeia. 1992: 468-473.

  • KomakS.CrosslandM.R. (2000): An assessment of the introduced mosquitofish (Gambusia affinis holbrooki) as a predator of eggs, hatchlings and tadpoles of native and non-native anurans. Wildlife Res. 27: 185-189.

  • LaneS.J.MahonyM.J. (2002): Larval anurans with synchronous and asynchronous development periods: contrasting responses to water reduction and predator presence. J. Anim. Ecol. 71: 780-792.

  • LimaS.L. (1998): Nonlethal effects in the ecology of predator-prey interactions. What are the ecological effects of anti-predator decision-making? Bioscience. 48: 25-34.

  • MargaritoraF.G.FerraraO.VagagginiD. (2001): Predatory impact of the mosquitofish (Gambusia holbrooki Girard) on zooplanktonic populations in a pond at Tenuta di Castelporziano (Rome, Central Italy). J. Limnol. 60: 189-193.

  • MarshD.M.BorrellB.J. (2001): Flexible oviposition strategies in túngara frogs and their implications for tadpole spatial distributions. Oikos. 93: 101-109.

  • McCueM.D. (2010): Starvation physiology. Comp. Biochem. Physiol. Part A. 156: 1-18.

  • MiaudC. (1993): Predation of newt eggs (Triturus alpestris and T. helveticus): identification of predators and protective role of oviposition behavior. J. Zool. 231: 575-582.

  • MiaudC. (1994): Role of wrapping behavior on egg survival in three species of Triturus (Amphibia: Urodela). Copeia. 1994: 535-537.

  • MorganL.A.ButtemerW.A. (1996): Predation by the non-native fish Gambusia holbrooki on small Litoria aurea and L. dentata tadpoles. Aust. Zool. 30: 143-149.

  • MortonR.M.BeumerJ.P.PollockB.R. (1988): Fishes of a subtropical Australian saltmarsh and their predation upon mosquitoes. Environ. Biol. Fish. 21: 185-194. DOI:10.1007/BF00004862.

  • NarayanE.J.CockremJ.F.HeroJ.-M. (2013): Sight of a predator induces a corticosterone stress response and generates fear in an amphibian. PLoS ONE 8: e73564. DOI:10.1371/journal.pone.0073564.

  • NesbitD.H.MeffeG.K. (1993): Cannibalism frequencies in wild populations of the Eastern Mosquitofish (Gambusia holbrooki: Poeciliidae) in South Carolina. Copeia. 3: 867-870.

  • OrizaolaG.BrañaF. (2003a): Oviposition behaviour and vulnerability of eggs to predation in four newt species (genus Triturus). Herpetol. J. 13: 121-124.

  • OrizaolaG.BrañaF. (2003b): Response of predator-naive newt larvae to food and predator presence. Canadian J. Zool. 81: 1845-1850.

  • OrizaolaG.BrañaF. (2003c): Do predator chemical cues affect oviposition site selection in newts? Herpetol. J. 13: 189-193.

  • Pérez-SantigosaN.Hidalgo-VilaJ.Díaz-PaniaguaC. (2003): Depredación y consumo de huevos de tritón pigmeo, Triturus pygmaeus, en los medios acuáticos temporales de Doñana. Rev. Esp. Herpetol. 17: 11-19.

  • PetrankaJ.W. (1983): Fish predation: a factor affecting the spatial distribution of a stream-breeding salamander. Copeia. 1983: 624-628.

  • PetrankaJ.W.HolbrookC.T. (2006): Wetland restoration for amphibians: should local sites be designed to support metapopulations or patchy populations? Restor. Ecol. 14: 404-411.

  • PollardC.J.StockwellM.P.BowerD.S.GarnhamJ.I.PickettE.J.DarcovichK.O’MearaJ.ClulowJ.MahonyM.J. (2017): Removal of an exotic fish influences amphibian breeding site selection. J. Wildl. Manag. 81: 720-727.

  • PykeG.H.WhiteA.W. (2000): Factors influencing predation on eggs and tadpoles of the endangered Green and Golden Bell Frog Litoria aurea by the introduced Plague Minnow Gambusia holbrooki. Aust. Zool. 31: 496-505.

  • R Core Development Team (2017): R: A Language and Environment for Statistical Computing. R Foundation for Statistical ComputingVienna.

  • RehageJ.S.BarnettB.K.Sih A. (2005): Foraging behaviour and invasiveness: do invasive Gambusia exhibit higher feeding rates and broader diets than their noninvasive relatives? Ecol. Freshw. Fish. 14: 352-360.

  • ReynoldsS.J. (2009): Impact of the introduced Poeciliid Gambusia holbrooki on amphibians in Southwestern Australia. Copeia. 2009: 296-302.

  • RiessenH.P. (1999): Predator-induced life history shifts in Daphnia: a synthesis of studies using meta-analysis. Can. J. Fish. Aquat. Sci. 56: 2487-2494.

  • SihA.BolnickD.I.LuttbegB.OrrockJ.L.PeacorS.D.PintorL.M.PreisserE.RehageJ.S.VoneshJ.R. (2010): Predator-prey naïveté, antipredator behavior, and the ecology of predator invasions. Oikos. 119: 610-621.

  • SpecziárA. (2004): Life history pattern and feeding ecology of introduced eastern mosquitofish, Gambusia holbrooki, in a thermal spa under temperate climate, of Lake Hévíz, Hungary. Hydrobiologia 522: 249-260.

  • StanbackM. (2010): Gambusia holbrooki predation on Pseudacris feriarum tadpoles. Herpetol. Conserv. Bio. 5: 486-489.

  • StrayerD.L. (2010): Alien species in fresh waters: ecological effects, interactions with other stressors, and prospects for the future. Freshwater Biol. 55: 152-174.

  • TóthZ. (2015): Context-dependent plastic response during egg-laying in a widespread newt species. PLoS ONE 10: e0138163. DOI:10.1371/journal.pone.0136044.

  • VermeijG.J. (1982): Unsuccessful predation and evolution. Amer. Naturalist. 120: 701-720.

  • VilàM.García-BerthouE. (2010): Monitoring biological invasions in freshwater habitats. In: Conservation Monitoring in Freshwater Habitats: a Practical Guide and Case Studies p. 91-100. HurfordC.SchneiderM.CowxI.G. Eds SpringerDordrecht, The Netherlands.

  • WassermanS.S. (1985): Oviposition behavior and its disruption in the Southern Cowpea Weevil, Callosobruchus maculatus F. (Coleoptera: Bruchidae). J. Econ. Entomol. 78: 89-92.

  • WatsonR.T.MathisA.ThompsonR. (2004): Influence of physical stress, distress cues, and predator kairomones on the foraging behavior of Ozark zigzag salamanders, Plethodon angusticlavius. Behav. Process. 65: 201-209.

  • WebbC.JossJ. (1997): Does predation by the fish Gambusia holbrooki (Atheriniformes: Poeciliidae) contribute to declining frog populations? Aust. Zool. 30: 316-324.

  • WellbornG.A.SkellyD.K.WernerE.E. (1996): Mechanisms creating community structure across a freshwater habitat gradient. Annu. Rev. Ecol. Syst. 27: 337-363.

  • WellsK.D. (2007): The Ecology and Behavior of Amphibians1st Edition. University of Chicago PressChicago.

  • WinandyL.DenoëlM. (2013a): Cues from introduced fish alter shelter use and feeding behaviour in adult Alpine newts. Ethology. 119: 121-129.

  • WinandyL.DenoëlM. (2013b): Introduced goldfish affect amphibians through inhibition of sexual behaviour in risky habitats: an experimental approach. PLoS ONE 8: e82736. DOI:10.1371/journal.pone.0082736.

  • WinandyL.DenoëlM. (2015): The aggressive personality of an introduced fish affects foraging behavior in a polymorphic newt. Behav. Ecol. 26: 1528-1536.

  • WinandyL.DarnetE.DenoëlM. (2015): Amphibians forgo aquatic life in response to alien fish introduction. Anim. Behav. 109: 209-216.

  • WinandyL.LegrandP.DenoëlM. (2017): Habitat selection and reproduction of newts in networks of fish and fishless aquatic patches. Anim. Behav. 123: 107-115.

  • XiongX.MichaudJ.P.LiX.WuP.ChuY.ZhangQ.LiuX. (2015): Chronic, predator-induced stress alters development and reproductive performance of the cotton bollworm, Helicoverpa armigera. BioControl. 60: 827-837.


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    Proportion (mean ± 1 SE) of gravid pygmy newts (Triturus pygmaeus) observed at different heights (bottom, medium or upper position) in the water column in three experimental treatments, with caged or free mosquitofish and with no fish.

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    Proportion (mean ± 1 SE) of worms consumed by gravid pygmy newts (Triturus pygmaeus) and mosquitofish (Gambusia holbrooki) during feeding trials included in three experimental treatments, with caged or free mosquitofish and with no fish.

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    Time (mean ± 1 SE) invested by gravid pygmy newts (Triturus pygmaeus) in capturing and consuming a worm within five minute trials included in three experimental treatments, with caged or free mosquitofish (Gambusia holbrooki) and with no fish.

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    Number (mean ± 1 SE) of wrapped and unwrapped eggs laid by gravid pygmy newts (Triturus pygmaeus) in three experimental treatments, with caged or free mosquitofish (Gambusia holbrooki) and with no fish.

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