Chemisch-analytische und elektrophoretische Untersuchungen an Seren von Uromastyx acanthinurus Bell, 1825 (Sauria: Agamidae)

In: Amphibia-Reptilia
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  • 1 Ameisgasse 63/4/12, A-1140 Wien, Österreich
  • 2 Ameisgasse 63/4/12, A-1140 Wien, Österreich

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Abstract

As only little information about the blood chemistry of reptiles is available, a contribution for the establishment of standard values of serum parameters from Uromastyx acanthinurus is given. The sera were tested for their contents of total protein, glucose, triglycerides, cholesterol, uric acid, urea, creatinine, bilirubin, aspartate aminotransferase, alanine aminotransferase, γ-glutamyl-transferase, iron, and calcium. In a countercurrent electrophoresis the serum proteins were separated according to their electrophoretic mobility. Additionally, the molecular masses of the proteins were determined by a discontinuous polyacrylamid gel electrophoresis in the presence of sodiumdodecylsulfate. By means of these techniques the albumin and the 7S-antibodies from the Spiny-tailed lizard sera were characterized.

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