Alarm cue specificity and response ontogeny in juvenile rainbow trout (Oncorhynchus mykiss)

in Behaviour
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Many aquatic prey animals release chemical cues upon being captured by a predator. These chemical cues, referred to as alarm cues, may act to warn nearby individuals of danger. For the cues to be useful, fish must be able to discern if they are indicative of a real threat; cues from conspecifics in different age groups may be irrelevant due to size- and habitat-related shifts in predation risk. We test the response of newly-hatched rainbow trout, Oncorhynchus mykiss, to three concentrations of alarm cues from conspecifics from two age groups: newly-hatched versus six-month-old juveniles. Newly-hatched trout demonstrated a significant fright response to all three concentrations of alarm cues, but showed no difference in strength of response based on either concentration or age of the cue donor. We propose that the newly-hatched trout did not respond differently because of the high risk of predation that they face during this life stage.



BehnkeR.J. (2002). Rainbow trout. — In: Trout and salmon of North America ( ScottG., ed.). The Free Press, New York, NY, p.  67-123.

BrownG.E.SmithR.J.F. (1997). Conspecific skin extracts elicit antipredator responses in juvenile rainbow trout (Oncorhynchus mykiss). — Can. J. Zool. 75: 1916-1922.

BrownG.E.LeBlancV.J.PorterL.E. (2001). Ontogenetic changes in the response of largemouth bass (Micropterus salmoides, Centrarchidae, Perciformes) to heterospecific alarm pheromones. — Ethology 107: 401-414.

BrownG.E.BongiornoT.DiCapuaD.M.IvanL.I.RohE. (2006). Effects of group size on the threat-sensitive response to varying concentrations of chemical alarm cues by juvenile convict cichlids. — Can. J. Zool. 84: 1-8.

Carreau-GreenN.D.MirzaR.S.MartinezM.L.PyleG.G. (2008). The ontogeny of chemically mediated antipredator responses of fathead minnows Pimephales promelas. — J. Fish. Biol. 73: 2390-2401.

ChiversD.P.SmithR.J.F. (1998). Chemical alarm signaling in aquatic predator-prey systems: a review and prospectus. — Ecoscience 5: 338-352.

FerrariM.C.O.MessierF.ChiversD.P. (2008). Can prey exhibit threat-sensitive generalization of predator recognition? Extending the predator recognition continuum hypothesis. — Proc. Roy. Soc. Lond. B: Biol. Sci. 275: 1811-1816.

GolubJ.L.BrownG.E. (2003). Are all signals the same? Ontogenetic change in the response to conspecific and heterospecific chemical alarm signals by juvenile green sunfish (Lepomis cyanellus). — Behav. Ecol. Sociobiol. 54: 113-118.

HelfmanG.S. (1989). Threat-sensitive predator avoidance in damselfish-trumpetfish interactions. — Behav. Ecol. Sociobiol. 24: 47-58.

HelfmanG.S.WinkelmanD.L. (1997). Threat sensitivity in bicolor damselfish: effects of sociality and body size. — Ethology 103: 369-383.

LawrenceB.J.SmithR.J.F. (1989). Behavioural response of solitary fathead minnows, Pimephales promelas, to alarm substance. — J. Chem. Ecol. 15: 209-219.

LimaS.L.DillL.M. (1990). Behavioral decisions made under the risk of predation: a review and prospectus. — Can. J. Zool. 68: 619-640.

LönnstedtO.M.McCormickM.I. (2011). Chemical alarm cues inform prey of predation threat: the importance of ontogeny and concentration in a coral reef fish. — Anim. Behav. 82: 213-218.

MarcusJ.BrownG.E. (2003). Response of pumpkinseed sunfish to conspecific chemical alarm cues: an interaction between ontogeny and stimulus concentration. — Can. J. Zool. 81: 1671-1677.

MirzaR.S.ChiversD.P. (2002). Brook char (Salvelinus fontinalis) can differentiate chemical alarm cues produced by different age/size classes of conspecifics. — J. Chem. Ecol. 28: 555-564.

MirzaR.S.ChiversD.P. (2003). Response of juvenile rainbow trout to varying concentrations of chemical alarm cue: response thresholds and survival during encounters with predators. — Can. J. Zool. 81: 88-95.

MitchellM.D.McCormickM.I. (2013). Ontogenetic differences in chemical alarm cue production determine antipredator responses and learned predator recognition. — Behav. Ecol. Sociobiol. 67: 1123-1129.

PaineR.T. (1976). Size-limited predation: an observational and experimental approach with the Mytilus-Pisaster interaction. — Ecology 57: 858-873.

PerssonL.AnderssonJ.WahlströmE.EklövP. (1996). Size-specific interactions in lake systems: predator gape limitation and prey growth rate and mortality. — Ecology 77: 900-911.

PfeifferW. (1963). The fright reaction in North American fish. — Can. J. Zool. 41: 69-77.

PfeifferW. (1977). The distribution of fright reaction and alarm substance cells in fishes. — Copeia: 653-665.

PopovaO.A. (1978). The role of predaceous fish in ecosystems. — In: Ecology of freshwater fish production ( GerkingS.D., ed.). Blackwell, Oxford, p.  215-249.

WaldmanB. (1982). Quantitative and developmental analyses of the alarm reaction in the zebra danio, Brachydanio rerio. — Copeia: 1-9.

WernerE.E.GilliamJ.F. (1984). The ontogenetic niche and species interactions in size-structured populations. — Annu. Rev. Ecol. Syst. 15: 393-425.

WisendenB.D. (2015). Chemical cues that indicate risk of predation. — In: Fish pheromones and related cues ( SorensenP.W.WisendenB.D., eds). Wiley-Blackwell, Chichester, p.  131-148.


  • Mean change ± SE in movement measured by line crosses by newly-hatched rainbow trout (Oncorhynchus mykiss) in response to distilled water (no AC) or alarm cue from newly-hatched (open) or 6-month-old (shaded bars) conspecifics at concentrations of 0.001, 0.01 or 0.1 cm2/ml. The asterisk denotes a significant difference in the Tukey’s LSD post-hoc test comparing concentrations (p0.02).

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