Effects of food matrix on the prebiotic efficacy of inulin-type fructans: a randomised trial
In: Beneficial MicrobesSearch for other papers by P.P.J. Jackson in
Current site
Google Scholar
Search for other papers by A. Wijeyesekera in
Current site
Google Scholar
Search for other papers by S. Theis in
Current site
Google Scholar
Search for other papers by J. Van Harsselaar in
Current site
Google Scholar
Search for other papers by R.A. Rastall in
Current site
Google Scholar
Abstract
Recently there is much debate in the scientific community over the impact of the food matrix on prebiotic efficacy of inulin-type fructans. Previous studies suggest that prebiotic selectivity of inulin-type fructans towards bifidobacteria is unaffected by the food matrix. Due to differences in study design, definitive conclusions cannot be drawn from these findings with any degree of certainty. In this randomised trial, we aimed to determine the effects that different food matrices had on the prebiotic efficacy of inulin-type fructans following a standardised 10-day, 4-arm, parallel, randomised protocol with inulin either in pure form or incorporated into shortbread biscuits, milk chocolate or a rice drink. Similar increases in Bifidobacterium counts were documented across all four interventions using both fluorescence in situ hybridisation (pure inulin: +0.63; shortbread: +0.59; milk chocolate: +0.65 and rice drink: +0.71 (log10 cells/g wet faeces) and 16S rRNA sequencing quantitative microbiome profiling data (pure inulin: +1.21 × 109; shortbread: +1.47 × 109; milk chocolate: +8.59 × 108 and rice drink: +1.04 × 109 (cells/g wet faeces) (all
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Aguilera, J.M., 2019. The food matrix: implications in processing, nutrition and health. Critical Reviews in Food Science and Nutrition 59: 3612-3629. https://doi.org/10.1080/10408398.2018.1502743
-
Ahmed, W. and Rashid, S., 2019. Functional and therapeutic potential of inulin: a comprehensive review. Critical Reviews in Food Science and Nutrition 59: 1-13. https://doi.org/10.1080/10408398.2017.1355775
-
Amann, R.I., Binder, B.J., Olson, R.J., Chisholm, S.W., Devereux, R. and Stahl, D.A., 1990. Combination of 16s rRNA-targeted oligonucleotide probes with flow-cytometry for analyzing mixed microbial-populations. Applied and Environmental Microbiology 56: 1919-1925. https://doi.org/10.1128/aem.56.6.1919-1925.1990
-
Azpiroz, F., Molne, L., Mendez, S., Nieto, A., Manichanh, C., Mego, M., Accarino, A., Santos, J., Sailer, M., Theis, S. and Guarner, F., 2017. Effect of chicory-derived inulin on abdominal sensations and bowel motor function. Journal of Clinical Gastroenterology 51: 619-625. https://doi.org/10.1097/mcg.0000000000000723
-
Brighenti, F., Casiraghi, M.C., Canzi, E. and Ferrari, A., 1999. Effect of consumption of a ready-to-eat breakfast cereal containing inulin on the intestinal milieu and blood lipids in healthy male volunteers. European Journal of Clinical Nutrition 53: 726-733. https://doi.org/10.1038/sj.ejcn.1600841
-
Buddington, R.K., Kapadia, C., Neumer, F. and Theis, S., 2017. Oligofructose provides laxation for irregularity associated with low fiber intake. Nutrients 9: 1372. https://doi.org/10.3390/nu9121372
-
Canani, R.B., Di Costanzo, M., Leone, L., Pedata, M., Meli, R. and Calignano, A., 2011. Potential beneficial effects of butyrate in intestinal and extrainitestinal diseases. World Journal of Gastroenterology 17: 1519-1528. https://doi.org/10.3748/wjg.v17.i12.1519
-
Costabile, A., Klinder, A., Fava, F., Napolitano, A., Fogliano, V., Leonard, C., Gibson, G.R. and Tuohy, K.M., 2008. Whole-grain wheat breakfast cereal has a prebiotic effect on the human gut microbiota: a double-blind, placebo-controlled, crossover study. British Journal of Nutrition 99: 110-120. https://doi.org/10.1017/s0007114507793923
-
Costabile, A., Kolida, S., Klinder, A., Gietl, E., Bauerlein, M., Frohberg, C., Landschutze, V. and Gibson, G.R., 2010. A double-blind, placebo-controlled, cross-over study to establish the bifidogenic effect of a very-long-chain inulin extracted from globe artichoke (Cynara scolymus) in healthy human subjects. British Journal of Nutrition 104: 1007-1017. https://doi.org/10.1017/s0007114510001571
-
Daims, H., Bruhl, A., Amann, R., Schleifer, K.H. and Wagner, M., 1999. The domain-specific probe EUB338 is insufficient for the detection of all bacteria: development and evaluation of a more comprehensive probe set. Systematic and Applied Microbiology 22: 434-444. https://doi.org/10.1016/s0723-2020(99)80053-8
-
D’Archivio, M., Filesi, C., Vari, R., Scazzocchio, B. and Masella, R., 2010. Bioavailability of the polyphenols: status and controversies. International Journal of Molecular Sciences 11: 1321-1342. https://doi.org/10.3390/ijms11041321
-
Dobranowski, P.A. and Stintzi, A., 2021. Resistant starch, microbiome, and precision modulation. Gut Microbes 13: 1926842. https://doi.org/10.1080/19490976.2021.1926842
-
Duar, R.M., Ang, P.T., Hoffman, M., Wehling, R., Hutkins, R. and Schlegel, V., 2015. Processing effects on four prebiotic carbohydrates supplemented in an extruded cereal and a low pH drink. Cogent Food and Agriculture 1: 1013782. https://doi.org/10.1080/23311932.2015.1013782
-
Falony, G., Lazidou, K., Verschaeren, A., Weckx, S., Maes, D. and De Vuyst, L., 2009. In vitro kinetic analysis of fermentation of prebiotic inulin-type fructans by Bifidobacterium species reveals four different phenotypes. Applied and Environmental Microbiology 75: 454-461. https://doi.org/10.1128/aem.01488-08
-
Franco, R.Z., Fallaize, R., Hwang, F. and Lovegrove, J.A., 2019. Strategies for online personalised nutrition advice employed in the development of the eNutri web app. Proceedings of the Nutrition Society 78: 407-417. https://doi.org/10.1017/s0029665118002707
-
François, I.E.J.A., Lescroart, O., Veraverbeke, W.S., Windey, K., Verbeke, K. and Broekaert, W.F., 2014. Tolerance and the effect of high doses of wheat bran extract, containing arabinoxylan-oligosaccharides, and oligofructose on faecal output: a double-blind, randomised, placebo-controlled, cross-over trial. Journal of Nutritional Science 3: e49. https://doi.org/10.1017/jns.2014.52
-
Franks, A.H., Harmsen, H.J.M., Raangs, G.C., Jansen, G.J., Schut, F. and Welling, G.W., 1998. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Applied and Environmental Microbiology 64: 3336-3345.
-
Gibson, G.R., Beatty, E.R., Wang, X. and Cummings, J.H., 1995. Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 108: 975-982. https://doi.org/10.1016/0016-5085(95)90192-2
-
Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Reimer, R.A., Salminen, S.J., Scott, K., Stanton, C., Swanson, K.S., Cani, P.D., Verbeke, K. and Reid, G., 2017. Expert consensus document: the international scientific association for probiotics and prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature Reviews Gastroenterology and Hepatology 14: 491-502. https://doi.org/10.1038/nrgastro.2017.75
-
Gibson, G.R. and Roberfroid, M.B., 1995. Dietary modulation of the human colonic microbiota – introducing the concept of prebiotics. Journal of Nutrition 125: 1401-1412. https://doi.org/10.1093/jn/125.6.1401
-
Glibowski, P. and Wasko, A., 2008. Effect of thermochemical treatment on the structure of inulin and its gelling properties. International Journal of Food Science and Technology 43: 2075-2082. https://doi.org/10.1111/j.1365-2621.2008.01825.x
-
Gomez, B., Gullon, B., Yanez, R., Schols, H. and Alonso, J.L., 2016. Prebiotic potential of pectins and pectic oligosaccharides derived from lemon peel wastes and sugar beet pulp: a comparative evaluation. Journal of Functional Foods 20: 108-121. https://doi.org/10.1016/j.jff.2015.10.029
-
Gressier, M. and Frost, G., 2022. Minor changes in fibre intake in the UK population between 2008/2009 and 2016/2017. European Journal of Clinical Nutrition 76: 322-327. https://doi.org/10.1038/s41430-021-00933-2
-
Grider, J.R. and Piland, B.E., 2007. The peristaltic reflex induced by short-chain fatty acids is mediated by sequential release of 5-HT and neuronal CGRP but not BDNF. American Journal of Physiology – Gastrointestinal and Liver Physiology 292: 429-437. https://doi.org/10.1152/ajpgi.00376.2006
-
Grimaldi, R., Cela, D., Swann, J.R., Vulevic, J., Gibson, G.R., Tzortzis, G. and Costabile, A., 2017. In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children. FEMS Microbiology Ecology 93: fiw233. https://doi.org/10.1093/femsec/fiw233
-
Harris, P.A., Taylor, R., Thielke, R., Payne, J., Gonzalez, N. and Conde, J.G., 2009. Research electronic data capture (REDCap)-a metadata-driven methodology and workflow process for providing translational research informatics support. Journal of Biomedical Informatics 42: 377-381. https://doi.org/10.1016/j.jbi.2008.08.010
-
Healey, G., Murphy, R., Butts, C., Brough, L., Whelan, K. and Coad, J., 2018. Habitual dietary fibre intake influences gut microbiota response to an inulin-type fructan prebiotic: a randomised, double-blind, placebo-controlled, cross-over, human intervention study. British Journal of Nutrition 119: 176-189. https://doi.org/10.1017/s0007114517003440
-
Isakov, V., Pilipenko, V., Shakhovskaya, A. and Tutelyan, V., 2013. Efficacy of inulin enriched yogurt on bowel habits in patients with irritable bowel syndrome with constipation: a pilot study. Faseb Journal 27 Suppl. 1: lb426.
-
Jackson, P.P.J., Wijeyesekera, A. and Rastall, R.A., 2023. Inulin-type fructans and short-chain fructooligosaccharides-their role within the food industry as fat and sugar replacers and texture modifiers-what needs to be considered! Food Science and Nutrition 11: 17-38. https://doi.org/10.1002/fsn3.3040
-
Jackson, P.P.J., Wijeyesekera, A., Theis, S., Van Harsselaar, J. and Rastall, R.A., 2022. Food for thought! Inulin-type fructans: does the food matrix matter? Journal of Functional Foods 90: 104987. https://doi.org/10.1016/j.jff.2022.104987
-
Karimi, R., Azizi, M.H., Ghasemlou, M. and Vaziri, M., 2015. Application of inulin in cheese as prebiotic, fat replacer and texturizer: a review. Carbohydrate Polymers 119: 85-100. https://doi.org/10.1016/j.carbpol.2014.11.029
-
Kemperman, R.A., Gross, G., Mondot, S., Possemiers, S., Marzorati, M., Van de Wiele, T., Dore, J. and Vaughan, E.E., 2013. Impact of polyphenols from black tea and red wine/grape juice on a gut model microbiome. Food Research International 53: 659-669. https://doi.org/10.1016/j.foodres.2013.01.034
-
Kim, H., Jeong, Y., Kang, S.N., You, H.J. and Ji, G.E., 2020. Co-culture with Bifidobacterium catenulatum improves the growth, gut colonization, and butyrate production of Faecalibacterium prausnitzii: in vitro and in vivo studies. Microorganisms 8: 788. https://doi.org/10.3390/microorganisms8050788
-
Kleessen, B., Schwarz, S., Boehm, A., Fuhrmann, H., Richter, A., Henle, T. and Krueger, M., 2007. Jerusalem artichoke and chicory inulin in bakery products affect faecal microbiota of healthy volunteers. British Journal of Nutrition 98: 540-549. https://doi.org/10.1017/s0007114507730751
-
Klewicki, R., 2007. The stability of gal-polyols and oligosaccharides during pasteurization at a low pH. Lwt-Food Science and Technology 40: 1259-1265. https://doi.org/10.1016/j.lwt.2006.08.008
-
Kruse, H.P., Kleessen, B. and Blaut, M., 1999. Effects of inulin on faecal bifidobacteria in human subjects. British Journal of Nutrition 82: 375-382. https://doi.org/10.1017/s0007114599001622
-
Langendijk, P.S., Schut, F., Jansen, G.J., Raangs, G.C., Kamphuis, G.R., Wilkinson, M.H.F. and Welling, G.W., 1995. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Applied and Environmental Microbiology 61: 3069-3075. https://doi.org/10.1128/aem.61.8.3069-3075.1995
-
Lewis, S.J. and Heaton, K.W., 1997. Stool form scale as a useful guide to intestinal transit time. Scandinavian Journal of Gastroenterology 32: 920-924. https://doi.org/10.3109/00365529709011203
-
Leylabadlo, H.E., Ghotaslou, R., Feizabadi, M.M., Farajnia, S., Moaddab, S.Y., Ganbarov, K., Khodadadi, E., Tanomand, A., Sheykhsaran, E., Yousefi, B. and Kafil, H.S., 2020. The critical role of Faecalibacterium prausnitzii in human health: an overview. Microbial Pathogenesis 149: 104344. https://doi.org/10.1016/j.micpath.2020.104344
-
Maki, K.C., Gibson, G.R., Dickmann, R.S., Kendall, C.W.C., Chen, C.Y.O., Costabile, A., Comelli, E.M., McKay, D.L., Almeida, N.G., Jenkins, D., Zello, G.A. and Blumberg, J.B., 2012. Digestive and physiologic effects of a wheat bran extract, arabino-xylan-oligosaccharide, in breakfast cereal. Nutrition 28: 1115-1121. https://doi.org/10.1016/j.nut.2012.02.010
-
Manz, W., Amann, R., Ludwig, W., Vancanneyt, M. and Schleifer, K.H., 1996. Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacter-Bacteroides in the natural environment. Microbiology 142: 1097-1106. https://doi.org/10.1099/13500872-142-5-1097
-
Marteau, P., Jacobs, H., Cazaubiel, M., Signoret, C., Prevel, J.M. and Housez, B., 2011. Effects of chicory inulin in constipated elderly people: a double-blind controlled trial. International Journal of Food Sciences and Nutrition 62: 164-170. https://doi.org/10.3109/09637486.2010.527323
-
Mensink, M.A., Frijlink, H.W., Maarschalk, K.V. and Hinrichs, W.L.J., 2015. Inulin, a flexible oligosaccharide I: review of its physicochemical characteristics. Carbohydrate Polymers 130: 405-419. https://doi.org/10.1016/j.carbpol.2015.05.026
-
Micka, A., Siepelmeyer, A., Holz, A., Theis, S. and Schon, C., 2017. Effect of consumption of chicory inulin on bowel function in healthy subjects with constipation: a randomized, double-blind, placebo-controlled trial. International Journal of Food Sciences and Nutrition 68: 82-89. https://doi.org/10.1080/09637486.2016.1212819
-
Murakami, K. and Livingstone, M.B.E., 2016. Energy density of meals and snacks in the British diet in relation to overall diet quality, BMI and waist circumference: findings from the National Diet and Nutrition Survey. British Journal of Nutrition 116: 1479-1489. https://doi.org/10.1017/s0007114516003573
-
Nagy, D.U., Sandor-Bajusz, K.A., Body, B., Decsi, T., Van Harsselaar, J., Theis, S. and Lohner, S., in press. Effect of chicory-derived inulin-type fructans on abundance of Bifidobacterium and on bowel function: a systematic review with meta-analyses. Critical Reviews in Food Science and Nutrition. https://doi.org/10.1080/10408398.2022.2098246
-
Palafox-Carlos, H., Ayala-Zavala, J.F. and Gonzalez-Aguilar, G.A., 2011. The role of dietary fiber in the bioaccessibility and bioavailability of fruit and vegetable antioxidants. Journal of Food Science 76: R6-R15. https://doi.org/10.1111/j.1750-3841.2010.01957.x
-
Pereira, G.V., Abdel-Hamid, A.M., Dutta, S., D’Alessandro-Gabazza, C.N., Wefers, D., Farris, J.A., Bajaj, S., Wawrzak, Z., Atomi, H., Mackie, R.I., Gabazza, E.C., Shukla, D., Koropatkin, N.M. and Cann, I., 2021. Degradation of complex arabinoxylans by human colonic Bacteroidetes. Nature Communications 12: 459. https://doi.org/10.1038/s41467-020-20737-5
-
Poinot, P., Arvisenet, G., Grua-Priol, J., Fillonneau, C., Le-Bail, A. and Prost, C., 2010. Influence of inulin on bread: kinetics and physico-chemical indicators of the formation of volatile compounds during baking. Food Chemistry 119: 1474-1484. https://doi.org/10.1016/j.foodchem.2009.09.029
-
Ramnani, P., Gaudier, E., Bingham, M., van Bruggen, P., Tuohy, K.M. and Gibson, G.R., 2010. Prebiotic effect of fruit and vegetable shots containing Jerusalem artichoke inulin: a human intervention study. British Journal of Nutrition 104: 233-240. https://doi.org/10.1017/s000711451000036x
-
Rao, V.A., 2001. The prebiotic properties of oligofructose at low intake levels. Nutrition Research 21: 843-848. https://doi.org/10.1016/s0271-5317(01)00284-6
-
Reimer, R.A., Soto-Vaca, A., Nicolucci, A.C., Mayengbam, S., Park, H., Madsen, K.L., Menon, R. and Vaughan, E.E., 2020. Effect of chicory inulin-type fructan-containing snack bars on the human gut microbiota in low dietary fiber consumers in a randomized crossover trial. American Journal of Clinical Nutrition 111: 1286-1296. https://doi.org/10.1093/ajcn/nqaa074
-
Ribas-Agusti, A., Martin-Belloso, O., Soliva-Fortuny, R. and Elez-Martinez, P., 2018. Food processing strategies to enhance phenolic compounds bioaccessibility and bioavailability in plant-based foods. Critical Reviews in Food Science and Nutrition 58: 2531-2548. https://doi.org/10.1080/10408398.2017.1331200
-
Riviere, A., Selak, M., Geirnaert, A., Van den Abbeele, P. and De Vuyst, L., 2018. Complementary mechanisms for degradation of inulin-type fructans and arabinoxylan oligosaccharides among bifidobacterial strains suggest bacterial cooperation. Applied and Environmental Microbiology 84. https://doi.org/10.1128/aem.02893-17
-
Riviere, A., Selak, M., Lantin, D., Leroy, F. and De Vuyst, L., 2016. Bifidobacteria and butyrate-producing colon bacteria: importance and strategies for their stimulation in the human gut. Frontiers in Microbiology 7: 979. https://doi.org/10.3389/fmicb.2016.00979
-
Roberfroid, M.B., 2007. Inulin-type fructans: functional food ingredients. Journal of Nutrition 137: 2493S-2502S.
-
Rodriguez-Garcia, J., Puig, A., Salvador, A. and Hernando, I., 2012. Optimization of a sponge cake formulation with inulin as fat replacer: structure, physicochemical, and sensory properties. Journal of Food Science 77: C189-C197. https://doi.org/10.1111/j.1750-3841.2011.02546.x
-
Rolim, P.M., 2015. Development of prebiotic food products and health benefits. Food Science and Technology 35: 3-10. https://doi.org/10.1590/1678-457x.6546
-
Sanders, M.E., Merenstein, D.J., Reid, G., Gibson, G.R. and Rastall, R.A., 2019. Probiotics and prebiotics in intestinal health and disease: from biology to the clinic. Nature Reviews Gastroenterology and Hepatology 16: 605-616. https://doi.org/10.1038/s41575-019-0173-3
-
Scientific Advisory Committee on Nutrition (SACN), 2015. Carbohydrates and health. TSO, London, UK.
-
Scott, K.P., Grimaldi, R., Cunningham, M., Sarbini, S.R., Wijeyesekera, A., Tang, M.L.K., Lee, J.C.Y., Yau, Y.F., Ansell, J., Theis, S., Yang, K., Menon, R., Arfsten, J., Manurung, S., Gourineni, V. and Gibson, G.R., 2019. Developments in understanding and applying prebiotics in research and practice-an ISAPP conference paper. Journal of Applied Microbiology 128: 934-949. https://doi.org/10.1111/jam.14424
-
Shoaib, M., Shehzad, A., Omar, M., Rakha, A., Raza, H., Sharif, H.R., Shakeel, A., Ansari, A. and Niazi, S., 2016. Inulin: properties, health benefits and food applications. Carbohydrate Polymers 147: 444-454. https://doi.org/10.1016/j.carbpol.2016.04.020
-
Slavin, J. and Feirtag, J., 2011. Chicory inulin does not increase stool weight or speed up intestinal transit time in healthy male subjects. Food and Function 2: 72-77. https://doi.org/10.1039/c0fo00101e
-
So, D., Whelan, K., Rossi, M., Morrison, M., Holtmann, G., Kelly, J.T., Shanahan, E.R., Staudacher, H.M. and Campbell, K.L., 2018. Dietary fiber intervention on gut microbiota composition in healthy adults: a systematic review and meta-analysis. American Journal of Clinical Nutrition 107: 965-983. https://doi.org/10.1093/ajcn/nqy041
-
Struyf, N., Van der Maelen, E., Hemdane, S., Verspreet, J., Verstrepen, K.J. and Courtin, C.M., 2017. Bread dough and baker’s yeast: an uplifting synergy. Comprehensive Reviews in Food Science and Food Safety 16: 850-867. https://doi.org/10.1111/1541-4337.12282
-
Suau, A., Rochet, V., Sghir, A., Gramet, G., Brewaeys, S., Sutren, M., Rigottier-Gois, L. and Dore, J., 2001. Fusobacterium prausnitzii and related species represent a dominant group within the human fecal flora. Systematic and Applied Microbiology 24: 139-145. https://doi.org/10.1078/0723-2020-00015
-
Thorning, T.K., Bertram, H.C., Bonjour, J.P., de Groot, L., Dupont, D., Feeney, E., Ipsen, R., Lecerf, J.M., Mackie, A., McKinley, M.C., Michalski, M.C., Remond, D., Riserus, U., Soedamah-Muthu, S.S., Tholstrup, T., Weaver, C., Astrup, A. and Givens, I., 2017. Whole dairy matrix or single nutrients in assessment of health effects: current evidence and knowledge gaps. American Journal of Clinical Nutrition 105: 1033-1045. https://doi.org/10.3945/ajcn.116.151548
-
Tuohy, K.M., Kolida, S., Lustenberger, A.M. and Gibson, G.R., 2001. The prebiotic effects of biscuits containing partially hydrolysed guar gum and fructo-oligosaccharides – a human volunteer study. British Journal of Nutrition 86: 341-348. https://doi.org/10.1079/bjn2001394
-
Valeur, J., Puaschitz, N.G., Midtvedt, T. and Berstad, A., 2016. Oatmeal porridge: impact on microflora-associated characteristics in healthy subjects. British Journal of Nutrition 115: 62-67. https://doi.org/10.1017/s0007114515004213
-
Van Loo, J., 2006. Inulin-type fructans as prebiotics. In: Gibson, G.R. and Rastall, R.A. (eds.) Prebiotics: development and application. John Wiley & Son, Chichester, UK, pp. 57-99.
-
Vandeputte, D., Kathagen, G., D’Hoe, K., Vieira-Silva, S., Valles-Colomer, M., Sabino, J., Wang, J., Tito, R.Y., De Commer, L., Darzi, Y., Ermeire, S.V., Falony, G. and Raes, J., 2017. Quantitative microbiome profiling links gut community variation to microbial load. Nature 551: 507. https://doi.org/10.1038/nature24460
-
Walker, A.W., Duncan, S.H., Leitch, E.C.M., Child, M.W. and Flint, H.J., 2005. PH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Applied and Environmental Microbiology 71: 3692-3700. https://doi.org/10.1128/aem.71.7.3692-3700.2005
-
Wallner, G., Amann, R. and Beisker, W., 1993. Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 14: 136-143. https://doi.org/10.1002/cyto.990140205
-
Walton, G.E., Lu, C.Y., Trogh, I., Arnaut, F. and Gibson, G.R., 2012. A randomised, double-blind, placebo controlled cross-over study to determine the gastrointestinal effects of consumption of arabinoxylan-oligosaccharides enriched bread in healthy volunteers. Nutrition Journal 11: 36. https://doi.org/10.1186/1475-2891-11-36
-
Wilson, B. and Whelan, K., 2017. Prebiotic inulin-type fructans and galacto-oligosaccharides: definition, specificity, function, and application in gastrointestinal disorders. Journal of Gastroenterology and Hepatology 32: 64-68. https://doi.org/10.1111/jgh.13700
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2399 | 949 | 77 |
| Full Text Views | 40 | 20 | 3 |
| PDF Views & Downloads | 66 | 37 | 6 |
Effects of food matrix on the prebiotic efficacy of inulin-type fructans: a randomised trial
In: Beneficial MicrobesSearch for other papers by P.P.J. Jackson in
Current site
Google Scholar
PubMed
Search for other papers by A. Wijeyesekera in
Current site
Google Scholar
PubMed
Search for other papers by S. Theis in
Current site
Google Scholar
PubMed
Search for other papers by J. Van Harsselaar in
Current site
Google Scholar
PubMed
Search for other papers by R.A. Rastall in
Current site
Google Scholar
PubMed
Abstract
Recently there is much debate in the scientific community over the impact of the food matrix on prebiotic efficacy of inulin-type fructans. Previous studies suggest that prebiotic selectivity of inulin-type fructans towards bifidobacteria is unaffected by the food matrix. Due to differences in study design, definitive conclusions cannot be drawn from these findings with any degree of certainty. In this randomised trial, we aimed to determine the effects that different food matrices had on the prebiotic efficacy of inulin-type fructans following a standardised 10-day, 4-arm, parallel, randomised protocol with inulin either in pure form or incorporated into shortbread biscuits, milk chocolate or a rice drink. Similar increases in Bifidobacterium counts were documented across all four interventions using both fluorescence in situ hybridisation (pure inulin: +0.63; shortbread: +0.59; milk chocolate: +0.65 and rice drink: +0.71 (log10 cells/g wet faeces) and 16S rRNA sequencing quantitative microbiome profiling data (pure inulin: +1.21 × 109; shortbread: +1.47 × 109; milk chocolate: +8.59 × 108 and rice drink: +1.04 × 109 (cells/g wet faeces) (all
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2399 | 949 | 77 |
| Full Text Views | 40 | 20 | 3 |
| PDF Views & Downloads | 66 | 37 | 6 |