Effect of orally-administered Lactobacillus plantarum LPLM-O1 strain in an immunosuppressed mouse model of urinary tract infection
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Abstract
Urinary tract infections (UTIs) affect both healthy and immunocompromised people, and they are treated with antibiotics. However, the high recurrence of UTIs obliges the use of natural mechanisms to regulate the normal microbiota through the use of e.g. lactic acid bacteria. In order to induce a UTI, 20 µl of the Escherichia coli (Ec-01) strain, in doses of 2.7×107 cfu/ml, was inoculated by way of the urethra in female Balb/c mice, all of them immunosuppressed with dexamethasone (10 mg/kg). Lactobacillus plantarum LPLM-O1 was used as a treatment,in daily doses of 1×107 cfu/ml, which were orally administered for seven days before the infection (preventive) or alongside the infection for seven days (curative). The oral administration of LPLM-O1 did not cause any adverse effects when used in an immunosuppressed animal model. It was observed that, when used as a preventive measure, LPLM-O1 induces a decrease in the infection, in the concentration of urinary leukocytes, and in the bacterial load. This study proposes the use of this lactic bacterium as a probiotic.
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-
Agace, W.W., Hedges, S.R., Ceska, M. and Svanborg, C., 1993. Interleukin-8 and the neutrophil response to mucosal Gram-negative infection. Journal of Clinical Investigation 92: 780-785.
'Interleukin-8 and the neutrophil response to mucosal Gram-negative infection ' () 92 Journal of Clinical Investigation : 780 -785.
-
Asahara, T., Nomoto, K., Watanuki, M. and Yokokura, T., 2001. Antimicrobial activity of intraurethrally administered probiotic Lactobacillus casei in a murine model of Escherichia coli urinary tract infection. Antimicrobial Agents and Chemotherapy 45: 1751-1760.
'Antimicrobial activity of intraurethrally administered probiotic Lactobacillus casei in a murine model of Escherichia coli urinary tract infection ' () 45 Antimicrobial Agents and Chemotherapy : 1751 -1760.
-
Berg, R.D. and Garlington, A., 1979. Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and others organs in a gnotobiotic mouse model. Infection and Immunity 23: 403-411.
'Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and others organs in a gnotobiotic mouse model ' () 23 Infection and Immunity : 403 -411.
-
Billips, B.K., Forrestal, S.G., Rycyk, M.T., Johnson, J.R., Klumpp, D.J. and Schaeffer, A.J., 2007. Modulation of host innate immune response in the bladder by uropathogenic Escherichia coli. Infection and Immunity 75: 5353-5360.
'Modulation of host innate immune response in the bladder by uropathogenic Escherichia coli ' () 75 Infection and Immunity : 5353 -5360.
-
Brooks, K.J., Bunce, K.T., Haase, M.V., White, A., Changani, K.K., Bate, S.T. and Reid, D.G., 2005. MRI quantification in vivo of corticosteroid induced thymus involution in mice: correlation with ex vivo measurements. Steroids 70: 267-272.
'MRI quantification in vivo of corticosteroid induced thymus involution in mice: correlation with ex vivo measurements ' () 70 Steroids : 267 -272.
-
Das, R.N., Chandrashekhar, T.S., Joshi, H.S., Gurung, M., Shrestha, N. and Shivananda, P.G., 2006. Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in Western Nepal. Singapore Medical Journal 47: 281-285.
'Frequency and susceptibility profile of pathogens causing urinary tract infections at a tertiary care hospital in Western Nepal ' () 47 Singapore Medical Journal : 281 -285.
-
Forman, B. and Brown, P., 2003. Epidemiology of urinary tract infections: transmission and risk factors, incidence, and costs. Infectious Disease Clinics of North America 17: 227-241.
'Epidemiology of urinary tract infections: transmission and risk factors, incidence, and costs ' () 17 Infectious Disease Clinics of North America : 227 -241.
-
Fraga, M., Scavone, P. and Zunino, P., 2005. Preventive and therapeutic administration of an indigenous Lactobacillus sp. strain against Proteus mirabilis ascending urinary tract infection in a mouse model. Antonie van Leeuwenhoek 88: 25-34.
'Preventive and therapeutic administration of an indigenous Lactobacillus sp ' () 88 strain against Proteus mirabilis ascending urinary tract infection in a mouse model. Antonie van Leeuwenhoek : 25 -34.
-
Gillon, G., Small, M., Medalia, O. and Aronson, M., 1984. Sequential study of bacterial clearance in experimental cystitis. Journal of Medical Microbiology 18: 319-326.
'Sequential study of bacterial clearance in experimental cystitis ' () 18 Journal of Medical Microbiology : 319 -326.
-
Hammes, W., Weiss, N. and Holzapfel, W., 1992. The genera Lactobacillus and Carnobacterium. In: Balow, A., Truper, H., Dworkin, M., Harder, W. and Schleifer, K.H. (eds). Prokaryotes, a handbook on the biology of bacteria: ecophysiology, isolation, applications. Springer Verlag, New York, NY, USA, pp. 1535-1573.
'The genera Lactobacillus and Carnobacterium ', in Prokaryotes, a handbook on the biology of bacteria: ecophysiology, isolation, applications , () 1535 -1573.
-
Haraoka, M., Hang, L., Frendéus, B., Godaly, G., Burdick, M., Strieter, R. and Svanborg, C., 1999. Neutrophil recruitment and resistance to urinary tract infection. Journal of Infectious Diseases 180: 1220-1229.
'Neutrophil recruitment and resistance to urinary tract infection ' () 180 Journal of Infectious Diseases : 1220 -1229.
-
Hashmi, S., Kelly, E., Rogers, S.O. and Gates, J., 2003. Urinary tract infection in surgical patients. American Journal of Surgery 186: 53-56.
'Urinary tract infection in surgical patients ' () 186 American Journal of Surgery : 53 -56.
-
Hedges, S., Anderson, P., Lidin-Janson, G., De Man, P. and Svanborg, C., 1991. Interleukin-6 response to deliberate colonization of the human urinary tract with Gram-negative bacteria. Infection and Immunology 59: 421-427.
'Interleukin-6 response to deliberate colonization of the human urinary tract with Gram-negative bacteria ' () 59 Infection and Immunology : 421 -427.
-
Hedges, S., Svensson, M. and Svanborg, C., 1992. Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro. Infection and Immunity 60: 1295-1301.
'Interleukin-6 response of epithelial cell lines to bacterial stimulation in vitro ' () 60 Infection and Immunity : 1295 -1301.
-
Hopkins, W.J., Gendron-Fitzpatrick, A., Balish, E. and Uehling, D.T., 1998. Time course and host responses to Escherichia coli urinary tract infection in genetically distinct mouse strains. Infection and Immunity 66: 2798-2802.
'Time course and host responses to Escherichia coli urinary tract infection in genetically distinct mouse strains ' () 66 Infection and Immunity : 2798 -2802.
-
Ishida-Fujii, K., Sato, R., Goto, S., Yang, X.P., Kuboki, H., Hirano, S. and Sato, M., 2007. Prevention of pathogenic Escherichia coli infection in mice and stimulation of macrophage activation in rats by an oral administration of probiotic Lactobacillus casei I-5. Bioscience, Biotechnology and Biochemistry 7: 866-873.
'Prevention of pathogenic Escherichia coli infection in mice and stimulation of macrophage activation in rats by an oral administration of probiotic Lactobacillus casei I-5 ' () 7 Bioscience, Biotechnology and Biochemistry : 866 -873.
-
James-Ellison, M., Roberts, R., Verrier-Jones, K., Williams, J. and Topley, N., 1997. Mucosa immunity in the urinary tract: changes in sIgA, FSC, and total IgA with age and in urinary tract infection. Clinical Nephrology 48: 69-78.
'Mucosa immunity in the urinary tract: changes in sIgA, FSC, and total IgA with age and in urinary tract infection ' () 48 Clinical Nephrology : 69 -78.
-
Jara, S., Sánchez, M., Vera, R., Cofré, J. and Castro, E., 2011. The inhibitory activity of Lactobacillus spp. isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin. Anaerobe 17: 474-477.
'The inhibitory activity of Lactobacillus spp ' () 17 isolated from breast milk on gastrointestinal pathogenic bacteria of nosocomial origin. Anaerobe : 474 -477.
-
Kandasamy, M., Jayasurya, A.S., Moochhala, S., Bay, B.H., Lee, Y.K. and Mahendran, R., 2011. Lactobacillus rhamnosus GG secreting an antigen and interleukin-2 translocates across the gastrointestinal tract and induces an antigen specific immune response. Microbiology and Immunology 55: 704-714.
'Lactobacillus rhamnosus GG secreting an antigen and interleukin-2 translocates across the gastrointestinal tract and induces an antigen specific immune response ' () 55 Microbiology and Immunology : 704 -714.
-
Korhonen, T.K., Virkola, R. and Holthöfer, H., 1986. Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney. Infection and Immunity 54: 328-332.
'Localization of binding sites for purified Escherichia coli P fimbriae in the human kidney ' () 54 Infection and Immunity : 328 -332.
-
Kunicka, J., Talle, M., Denhardt, G., Brown, M., Prince, L. and Goldstein, G., 1993. Immunosuppression by glucocorticoids: inhibition of production of multiple lymphokynes by in vivo administration of dexamethasone. Cellular Immunology 149: 39-49.
'Immunosuppression by glucocorticoids: inhibition of production of multiple lymphokynes by in vivo administration of dexamethasone ' () 149 Cellular Immunology : 39 -49.
-
Lin, W.-H, Yu, B., Lin, C.K., Hwang, W.Z. and Tsen, H.Y., 2007. Immune effect of heat-killed multistrain of Lactobacillus acidophilus against Salmonella typhimurium invasion to mice. Journal of Applied Microbiology 102: 22-31.
'Immune effect of heat-killed multistrain of Lactobacillus acidophilus against Salmonella typhimurium invasion to mice ' () 102 Journal of Applied Microbiology : 22 -31.
-
Maeda, N., Nakamura, R., Hirose, Y., Murosaki, S., Yamamoto, Y., Kase, T. and Yoshikai, Y., 2009. Oral administration of heat-killed Lactobacillus plantarum L-137 enhances protection against influenza virus infection by stimulation of type I interferon production in mice. International Immunopharmacology 9: 1122-1125.
'Oral administration of heat-killed Lactobacillus plantarum L-137 enhances protection against influenza virus infection by stimulation of type I interferon production in mice ' () 9 International Immunopharmacology : 1122 -1125.
-
McGroarty, J.A. and Reid, G., 1988. Detection of a Lactobacillus substance that inhibits Escherichia coli. Canadian Journal of Microbiology 34: 974-978.
'Detection of a Lactobacillus substance that inhibits Escherichia coli ' () 34 Canadian Journal of Microbiology : 974 -978.
-
Niers, L., Timmerman, H., Rijkers, G., Van Bleek, G., Van Uden, N., Knol, E., Kapsenberg, M., Kimpen, J., and Hoekstra, M., 2005. Identification of strong interleukin-10 inducing lactic acid bacteria which down-regulate T helper type 2 cytokines. Clinical and Experimental Allergy 35: 1481-1489.
'Identification of strong interleukin-10 inducing lactic acid bacteria which down-regulate T helper type 2 cytokines ' () 35 Clinical and Experimental Allergy : 1481 -1489.
-
Ocaña, V. and Nader-Macias, M., 2001. Adhesion of vaginal Lactobacillus strains with probiotic properties to vaginal epithelial cells. Biocell 25: 121-129.
'Adhesion of vaginal Lactobacillus strains with probiotic properties to vaginal epithelial cells ' () 25 Biocell : 121 -129.
-
Reid, G. and Bruce, A., 2006. Probiotics to prevent urinary tract infections: the rationale and evidence. World Journal of Urology 24: 28-32.
'Probiotics to prevent urinary tract infections: the rationale and evidence ' () 24 World Journal of Urology : 28 -32.
-
Reid, G., McGroarty, J., Angotti, R. and Cook, R., 1988. Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens. Canadian Journal of Microbiology 34: 344-351.
'Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens ' () 34 Canadian Journal of Microbiology : 344 -351.
-
Rosser, C., Bare, R. and Meredith, J., 1999. Urinary tract infection in the critically ill patient with a urinary catheter. The American Journal of Surgery 117: 287-290.
'Urinary tract infection in the critically ill patient with a urinary catheter ' () 117 The American Journal of Surgery : 287 -290.
-
Schiffrin, E., Rochat, F., Link-Amster, H., Aeschlimann, J. and Donnet-Hughes, A., 1995. Inmunomodulation of human blood cells following the ingestion of lactic acid bacteria. Journal of Dairy Science 78: 491-497.
'Inmunomodulation of human blood cells following the ingestion of lactic acid bacteria ' () 78 Journal of Dairy Science : 491 -497.
-
Thumbikat, P., Waltenbaugh, C., Schaeffer, A. and Klumpp, D., 2006. Antigen-specific responses accelerate bacterial clearance in the bladder. Journal of Immunology 176: 3080-3086.
'Antigen-specific responses accelerate bacterial clearance in the bladder ' () 176 Journal of Immunology : 3080 -3086.
-
Turner, A., Shao-Nong, C., Joike, M.K., Pendland, S.L., Pauli, G.F. and Farnsworth, N.R., 2005. Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay. Journal of Agricultural and Food Chemistry 53: 8940-8947.
'Inhibition of uropathogenic Escherichia coli by cranberry juice: a new antiadherence assay ' () 53 Journal of Agricultural and Food Chemistry : 8940 -8947.
-
Valdivieso, F., Trucco, O., Prado, V., Díaz, M.A. and Ojeda, A., 1999. Resistencia a los antimicrobianos en agentes causantes de infección del tracto urinario en 11 hospitales chilenos: proyecto PRONARES. Revista Médica de Chile 127: 1033-1040.
'Resistencia a los antimicrobianos en agentes causantes de infección del tracto urinario en 11 hospitales chilenos: proyecto PRONARES ' () 127 Revista Médica de Chile : 1033 -1040.
-
Von der Weid, T., Bulliard, C. and Schiffrin, E., 2001. Induction by lactic acid bacteria of a population of CD4+ T cells with low proliferative capacity that produce transforming growth factor β and interleukin-10. Clinical and Diagnostic Laboratory Immunology 8: 695-701.
'Induction by lactic acid bacteria of a population of CD4+ T cells with low proliferative capacity that produce transforming growth factor β and interleukin-10 ' () 8 Clinical and Diagnostic Laboratory Immunology : 695 -701.
-
Williams, D. and Schaeffer, A., 2004. Current concepts in urinary tract infections. Minerva Urologica e Nefrologica 56: 15-31.
'Current concepts in urinary tract infections ' () 56 Minerva Urologica e Nefrologica : 15 -31.
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Effect of orally-administered Lactobacillus plantarum LPLM-O1 strain in an immunosuppressed mouse model of urinary tract infection
In: Beneficial MicrobesSearch for other papers by A. Ramírez de Arellano in
Current site
Google Scholar
PubMed
Search for other papers by M. Sánchez in
Current site
Google Scholar
PubMed
Search for other papers by R. Vera in
Current site
Google Scholar
PubMed
Search for other papers by S. Jara in
Current site
Google Scholar
PubMed
Search for other papers by M. González in
Current site
Google Scholar
PubMed
ercastro@udec.cl
Search for other papers by E. Castro in
Current site
Google Scholar
PubMed
Abstract
Urinary tract infections (UTIs) affect both healthy and immunocompromised people, and they are treated with antibiotics. However, the high recurrence of UTIs obliges the use of natural mechanisms to regulate the normal microbiota through the use of e.g. lactic acid bacteria. In order to induce a UTI, 20 µl of the Escherichia coli (Ec-01) strain, in doses of 2.7×107 cfu/ml, was inoculated by way of the urethra in female Balb/c mice, all of them immunosuppressed with dexamethasone (10 mg/kg). Lactobacillus plantarum LPLM-O1 was used as a treatment,in daily doses of 1×107 cfu/ml, which were orally administered for seven days before the infection (preventive) or alongside the infection for seven days (curative). The oral administration of LPLM-O1 did not cause any adverse effects when used in an immunosuppressed animal model. It was observed that, when used as a preventive measure, LPLM-O1 induces a decrease in the infection, in the concentration of urinary leukocytes, and in the bacterial load. This study proposes the use of this lactic bacterium as a probiotic.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 105 | 105 | 5 |
| Full Text Views | 9 | 2 | 0 |
| PDF Views & Downloads | 4 | 4 | 0 |