Save

Transient inflammatory-like state and microbial dysbiosis are pivotal in establishment of mucosal homeostasis during colonisation of germ-free mice

In: Beneficial Microbes
Authors:
S. El Aidy

Search for other papers by S. El Aidy in
Current site
Google Scholar
PubMed
Close
,
M. Derrien

Search for other papers by M. Derrien in
Current site
Google Scholar
PubMed
Close
,
R. Aardema

Search for other papers by R. Aardema in
Current site
Google Scholar
PubMed
Close
,
G. Hooiveld Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

Search for other papers by G. Hooiveld in
Current site
Google Scholar
PubMed
Close
,
S.E. Richards School of Science and Technology, Nottingham Trent University, Clifton Lane A453, Nottingham NG11 8NS, United Kingdom

Search for other papers by S.E. Richards in
Current site
Google Scholar
PubMed
Close
,
A. Dane

Search for other papers by A. Dane in
Current site
Google Scholar
PubMed
Close
,
J. Dekker

Search for other papers by J. Dekker in
Current site
Google Scholar
PubMed
Close
,
R. Vreeken

Search for other papers by R. Vreeken in
Current site
Google Scholar
PubMed
Close
,
F. Levenez Commensal and Food Bacteria Group, INRA, UMR1319 Micalis, Domaine de Vilvert, 78350 Jouy-en-Josas, France

Search for other papers by F. Levenez in
Current site
Google Scholar
PubMed
Close
,
J. Doré Commensal and Food Bacteria Group, INRA, UMR1319 Micalis, Domaine de Vilvert, 78350 Jouy-en-Josas, France

Search for other papers by J. Doré in
Current site
Google Scholar
PubMed
Close
,
E.G. Zoetendal

Search for other papers by E.G. Zoetendal in
Current site
Google Scholar
PubMed
Close
,
P. van Baarlen Department of Animal Sciences, Host Microbe Interactomics Group, Wageningen University, De Elst 1, 6708 WD Wageningen, the Netherlands

Search for other papers by P. van Baarlen in
Current site
Google Scholar
PubMed
Close
, and
M. Kleerebezem

Search for other papers by M. Kleerebezem in
Current site
Google Scholar
PubMed
Close
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

$40.00

The gut microbiota is increasingly recognised as a key-player in defining the health status of the gastrointestinal tract. Recently, we demonstrated that colonisation of healthy germfree mice with a conventional microbiota (conventionalisation) elicits temporal and region specific host-microbe communication responses that lead to the establishment of a microbiota-accommodating homeostatic state within 30 days. Here, the microbiota composition profiles, mucosal transcriptomes and plasma-analytes in germ-free and conventionalised C57/BL 6 J mice were assessed to decipher the features of the distinctive and pivotal events occurring four days after initiation of the conventionalisation process. The dominance of the microbial genera Helicobacter, Sphingomonas and Mucispirillum in the gut microbiota coincided with the transient mounting of proinflammatory responses in the mucosa and the transiently elevated levels of specific (inflammatory) cytokines and amines in plasma. The overrepresented microbes have previously been associated with the potential to cause disease under certain conditions, illustrating that conventionalisation proceeds through a transient state that resembles situations associated with dysbiosis. However, no overt mucosal inflammation was observed, suggesting a pivotal role of the overrepresented bacterial groups in priming and maturation of the immune system during the process of conventionalisation. These findings imply that the transiently elevated relative overgrowth of particular microbial genera functions as pivotal adjuvants to elicit the corresponding proinflammatory cascades, which precede the full maturation of the different arms of the immune system following these events and is required to achieve a microbiota-accommodating homeostasis in healthy animals.

Content Metrics

All Time Past 365 days Past 30 Days
Abstract Views 283 202 20
Full Text Views 63 56 15
PDF Views & Downloads 21 12 0