Kale, a cultivar of Brassica oleracea, has attracted a great deal of attention because of its health-promoting effects, which are thought to be exerted through modulation of the intestinal microbiota. The present study was performed to investigate the effects of kale ingestion on the gastrointestinal microbial ecology of mice. 21 male C57BL/6J mice were divided into three groups and housed in a specific pathogen-free facility. The animals were fed either a control diet or experimental diets supplemented with different commercial kale products for 12 weeks. Contents of the caecum and colon of the mice were processed for the determination of active bacterial populations by a bacterial rRNA-based quantification method and short-chain fatty acids by HPLC. rRNAs of Bacteroides-Prevotella, the Clostridium coccoides-Eubacterium rectale group, and Clostridium leptum subgroup constituted the major fraction of microbiota regardless of the composition of the diet. The ratio of Firmicutes to Bacteroidetes was higher in the colon samples of one of the kale diet groups than in the control. The colonic butyrate level was also higher with the kale-supplemented diet. Overall, the ingestion of kale tended to either increase or decrease the activity of specific bacterial groups in the mouse gastrointestinal tract, however, the effect might vary depending on the nutritional composition.
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Abnous, K., Brooks, S.P.J., Kwan, J., Matias, F., Green-Johnson, J., Selinger, L.B., Thomas, M. and Kalmokoff, M., 2009. Diets enriched in oat bran or wheat bran temporally and differentially alter the composition of the fecal community of rats. Journal of Nutrition 139: 2024-2031.
'Diets enriched in oat bran or wheat bran temporally and differentially alter the composition of the fecal community of rats ' () 139 Journal of Nutrition : 2024 -2031.
Duncan, S.H., Hold, G.L., Barcenilla, A., Stewart, C.S. and Flint, H.J., 2002. Roseburia intestinalis sp. nov., a novel saccharolytic, butyrate-producing bacterium from human faeces. International Journal of Systematic and Evolutionary Microbiology 52: 1615-1620.
'Roseburia intestinalis sp. nov., a novel saccharolytic, butyrate-producing bacterium from human faeces ' () 52 International Journal of Systematic and Evolutionary Microbiology : 1615 -1620.
Flint, H.J., 2011. Obesity and the gut microbiota. Journal of Clinical Gastroenterology 45: S128-S132.
'Obesity and the gut microbiota ' () 45 Journal of Clinical Gastroenterology : S128 -S132.
Hildebrandt, M.A., Hoffmann, C., Sherrill-Mix, S.A., Keilbaugh, S.A., Hamady, M., Chen, Y.Y., Knight, R., Ahima, R.S., Bushman, F. and Wu, G.D., 2009. High-fat diet determines the composition of the murine gut microbiome independently of obesity. Gastroenterology 137: 1716-1724.
'High-fat diet determines the composition of the murine gut microbiome independently of obesity ' () 137 Gastroenterology : 1716 -1724.
Ji, Y., Kim, H., Park, H., Lee, J., Yeo, S., Yang, J., Park, S., Yoon, H., Cho, G., Franz, C., Bomba, A., Shin, H. and Holzapfel, W., 2012. Modulation of the murine microbiome with a concomitant anti-obesity effect by Lactobacillus rhamnosus GG and Lactobacillus sakei NR28. Beneficial Microbes 3: 13-22.
'Modulation of the murine microbiome with a concomitant anti-obesity effect by Lactobacillus rhamnosus GG and Lactobacillus sakei NR28 ' () 3 Beneficial Microbes : 13 -22.
Jumpertz, R., Le, D.S., Turnbaugh, P.J., Trinidad, C., Bogardus, C., Gordon, J.I. and Krakoff, J., 2011. Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans. American Journal of Clinical Nutrition 94: 58-65.
'Energy-balance studies reveal associations between gut microbes, caloric load, and nutrient absorption in humans ' () 94 American Journal of Clinical Nutrition : 58 -65.
Louis, P. and Flint, H.J., 2009. Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine. FEMS Microbiology letters 294: 1-8.
'Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine ' () 294 FEMS Microbiology letters : 1 -8.
Miyamoto, M., Seto, Y., Hai Hao, D., Teshima, T., Bo Sun, Y., Kabuki, T., Bing Yao, L. and Nakajima, H., 2005. Lactobacillus harbinensis sp. nov., consisted of strains isolated from traditional fermented vegetables ‘Suan cai’ in Harbin, Northeastern China and Lactobacillus perolens DSM 12745. Systematic and Applied Microbiology 28: 688-694.
'Lactobacillus harbinensis sp. nov., consisted of strains isolated from traditional fermented vegetables ‘Suan cai’ in Harbin, Northeastern China and Lactobacillus perolens DSM 12745 ' () 28 Systematic and Applied Microbiology : 688 -694.
Mullaney, J.A., Kelly, W.J., McGhie, T.K., Ansell, J. and Heyes, J.A., 2013. Lactic acid bacteria convert glucosinolates to nitriles efficiently yet differently from Enterobacteriaceae. Journal of Agricultural and Food Chemistry 61: 3039-3046.
'Lactic acid bacteria convert glucosinolates to nitriles efficiently yet differently from Enterobacteriaceae ' () 61 Journal of Agricultural and Food Chemistry : 3039 -3046.
Nilsson, J., Olsson, K., Engqvist, G., Ekvall, J., Olsson, M., Nyman, M. and Åkesson, B., 2006. Variation in the content of glucosinolates, hydroxycinnamic acids, carotenoids, total antioxidant capacity and low-molecular-weight carbohydrates in Brassica vegetables. Journal of the Science of Food and Agriculture 86: 528-538.
'Variation in the content of glucosinolates, hydroxycinnamic acids, carotenoids, total antioxidant capacity and low-molecular-weight carbohydrates in Brassica vegetables ' () 86 Journal of the Science of Food and Agriculture : 528 -538.
Schwiertz, A., Taras, D., Schäfer, K., Beijer, S., Bos, N.A., Donus, C. and Hardt, P.D., 2010. Microbiota and SCFA in lean and overweight healthy subjects. Obesity 18: 190-195.
'Microbiota and SCFA in lean and overweight healthy subjects ' () 18 Obesity : 190 -195.
Selma, M.V., Espín, J.C. and Tomás-Barberán, F.A., 2009. Interaction between phenolics and gut microbiota: role in human health. Journal of Agricultural and Food Chemistry 57: 6485-6501.
'Interaction between phenolics and gut microbiota: role in human health ' () 57 Journal of Agricultural and Food Chemistry : 6485 -6501.
Shen, J., Obin, M.S. and Zhao, L., 2013. The gut microbiota, obesity and insulin resistance. Molecular Aspects of Medicine 34: 39-58.
'The gut microbiota, obesity and insulin resistance ' () 34 Molecular Aspects of Medicine : 39 -58.
Uyeno, Y., Kawashima, K., Hasunuma, T., Wakimoto, W., Noda, M., Nagashima, S., Akiyama, K., Tabata, M. and Kushibiki, S., 2013. Effects of cellooligosaccharide or a combination of cellooligosaccharide and live Clostridium butyricum culture on performance and intestinal ecology in Holstein calves fed milk or milk replacer. Livestock Science 153: 88-93.
'Effects of cellooligosaccharide or a combination of cellooligosaccharide and live Clostridium butyricum culture on performance and intestinal ecology in Holstein calves fed milk or milk replacer ' () 153 Livestock Science : 88 -93.
Uyeno, Y., Sekiguchi, Y. and Kamagata, Y., 2008. Impact of consumption of probiotic lactobacilli-containing yogurt on microbial composition in human feces. International Journal of Food Microbiology 122: 16-22.
'Impact of consumption of probiotic lactobacilli-containing yogurt on microbial composition in human feces ' () 122 International Journal of Food Microbiology : 16 -22.
Uyeno, Y., Sekiguchi, Y. and Kamagata, Y., 2010. rRNA-based analysis to monitor succession of faecal bacterial communities in Holstein calves. Letters in Applied Microbiology 51: 570-577.
'rRNA-based analysis to monitor succession of faecal bacterial communities in Holstein calves ' () 51 Letters in Applied Microbiology : 570 -577.
Williams, D.J., Edwards, D., Hamernig, I., Jian, L., James, A.P., Johnson, S.K. and Tapsell, L.C., 2013. Vegetables containing phytochemicals with potential anti-obesity properties: a review. Food Research International 52: 323-333.
'Vegetables containing phytochemicals with potential anti-obesity properties: a review ' () 52 Food Research International : 323 -333.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 176 | 137 | 16 |
Full Text Views | 17 | 3 | 0 |
PDF Views & Downloads | 13 | 5 | 0 |
Kale, a cultivar of Brassica oleracea, has attracted a great deal of attention because of its health-promoting effects, which are thought to be exerted through modulation of the intestinal microbiota. The present study was performed to investigate the effects of kale ingestion on the gastrointestinal microbial ecology of mice. 21 male C57BL/6J mice were divided into three groups and housed in a specific pathogen-free facility. The animals were fed either a control diet or experimental diets supplemented with different commercial kale products for 12 weeks. Contents of the caecum and colon of the mice were processed for the determination of active bacterial populations by a bacterial rRNA-based quantification method and short-chain fatty acids by HPLC. rRNAs of Bacteroides-Prevotella, the Clostridium coccoides-Eubacterium rectale group, and Clostridium leptum subgroup constituted the major fraction of microbiota regardless of the composition of the diet. The ratio of Firmicutes to Bacteroidetes was higher in the colon samples of one of the kale diet groups than in the control. The colonic butyrate level was also higher with the kale-supplemented diet. Overall, the ingestion of kale tended to either increase or decrease the activity of specific bacterial groups in the mouse gastrointestinal tract, however, the effect might vary depending on the nutritional composition.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 176 | 137 | 16 |
Full Text Views | 17 | 3 | 0 |
PDF Views & Downloads | 13 | 5 | 0 |