Save

In vitro protective effect of lactic acid bacteria on Listeria monocytogenes adhesion and invasion of Caco-2 cells

In: Beneficial Microbes
Authors:
L.K. Winkelströter Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brazil

Search for other papers by L.K. Winkelströter in
Current site
Google Scholar
PubMed
Close
and
E.C.P. De Martinis Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (FCFRP-USP), Av. do Café s/n, 14040-903 Ribeirão Preto, SP, Brazil

Search for other papers by E.C.P. De Martinis in
Current site
Google Scholar
PubMed
Close
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

$40.00

The adhesion of Listeria monocytogenes to intestinal endothelial cells is a crucial step in the infection process, which is not well understood. In this study, we evaluated the potential ability of bacteriocin-producing Enterococcus faecium, Leuconostoc mesenteroides and Lactobacillus sakei strains to prevent the adhesion and invasion of eukaryotic cells by ten different L. monocytogenes isolates. The results showed that E. faecium 130 co-cultured with L. monocytogenes was the most effective in preventing infection of Caco-2 cells, as the vast majority of isolates showed significantly lower adhesion counts and invasion rates below the quantification limit of the method (<30 cfu/plate). L. sakei 1 was the least effective strain in preventing L. monocytogenes infection; only one isolate presented a lower adhesion rate and two isolates reduced the invasion rate of Caco-2 cells. Fluorescence in situ hybridisation (FISH) assay was shown to be an effective tool to illustrate and identify species in co-culture with L. monocytogenes during the adhesion process to Caco-2 cells.

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 43 42 3
Full Text Views 4 2 0
PDF Views & Downloads 1 1 0