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Potentially probiotic Lactobacillus strains with anti-proliferative activity induce cytokine/chemokine production and neutrophil recruitment in mice

In: Beneficial Microbes
Authors:
G. Saxami Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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A. Karapetsas Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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P. Chondrou Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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S. Vasiliadis Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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E. Lamprianidou Department of Hematology, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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I. Kotsianidis Department of Hematology, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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P. Ypsilantis Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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S. Botaitis Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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C. Simopoulos Laboratory of Experimental Surgery and Surgical Research, Medical School, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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A. Galanis Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.

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Lactobacillus pentosus B281 and Lactobacillus plantarum B282 are two Lactobacillus strains previously isolated from fermented table olives. Both strains were found to possess probiotic properties and displayed desirable technological characteristics for application as starters in novel functional food production. In the present study the anti-proliferative and immunostimulatory activities of the two strains were investigated. Firstly, we demonstrated that live L. pentosus B281 and L. plantarum B282 significantly inhibited the growth of human colon cancer cells (Caco-2) in a time- and dose-dependent manner. By employing the air pouch system in mice, we showed that administration of both strains led to a rapid and statistically significant infiltration of leukocytes in the air pouch exudates. The phenotypical characterisation of the recruited immune cells was performed by flow cytometry analysis. We demonstrated that the majority of the infiltrated leukocytes were neutrophils. Finally by using the Mouse Cytokine Array Panel A Detection Antibody cocktail, we showed that both strains induced the expression of granulocyte-colony stimulating factor, interleukin (IL)-1α, IL-1β, IL-6, chemokine (C-X-C motif) ligand (CXCL)-1, chemokine (C-C motif) ligand (CCL)-3, CCL-4, and CXCL-2 and diminished the expression levels of soluble intercellular adhesion molecule, macrophage colony-stimulating factor and metallopeptidase inhibitor 1. Our results showed that both strains display anti-proliferative and immunostimulatory properties equal or even better in some cases than those of established and commonly used probiotic strains. These findings further support the probiotic character of the two strains.

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