Heat-treated lactic acid bacteria is added to some foods because it is easier to store and transport, and have less interference with other food ingredient compared with living lactic acid bacteria. We have reported that oral administration of heat-treated Lactobacillus crispatus KT-11 strain (KT-11) suppressed the symptoms of periodontal disease in mice orally infected with Porphyromonas gingivalis. However, the preventive effect of KT-11 on periodontal disease in human is unclear. The aim of this randomised double-blind clinical trial was to examine the effects of KT-11 consumption on the oral environment in healthy volunteers. Sixteen healthy volunteers were randomly assigned to two groups (KT-11 or placebo), and they consumed a KT-11 food tablet (1.2×1010 KT-11 cells) or a placebo food tablet (without KT-11) every day for 4 weeks. Clinical parameters including numbers of major periodontopathic bacteria in saliva, plaque score, gum conditions, and oral mucosal fluid level were evaluated at weeks 0 and 4. Amount of change in P. gingivalis numbers decreased in the KT-11 group compared to that in the placebo group at week 4. Female participants in the KT-11 group had decreased plaque scores, reddish tinge, and gingival swelling scores compared to those in the placebo group at week 4. Furthermore, male participants in the KT-11 group demonstrated increased oral mucosa fluid scores. These clinical findings suggest that daily KT-11 intake can prevent periodontal disease through the improvement of oral conditions.
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Beck, J., Garcia, R., Heiss, G., Vokonas, P.S. and Offenbacher, S., 1996. Periodontal disease and cardiovascular disease. Journal of Periodontology 67: 1123-1137.
'Periodontal disease and cardiovascular disease ' () 67 Journal of Periodontology : 1123 -1137.
Brandtzaeg, P., 1997. Homing of mucosal immune cells – a possible connection between intestinal and articular inflammation. Alimentary Pharmacology and Therapeutics 11: 24-39.
'Homing of mucosal immune cells – a possible connection between intestinal and articular inflammation ' () 11 Alimentary Pharmacology and Therapeutics : 24 -39.
Carrouel, F., Viennot, S., Santamaria, J., Veber, P. and Bourgeois, D., 2016. Quantitative molecular detection of 19 major pathogens in the interdental biofilm of periodontally healthy young adults. Frontiers in Microbiology 7: 840.
'Quantitative molecular detection of 19 major pathogens in the interdental biofilm of periodontally healthy young adults ' () 7 Frontiers in Microbiology : 840.
Chałas, R., Wójcik-Chęcińska, I., Woźniak, M.J., Grzonka, J., Święszkowski, W. and Kurzydłowski, K.J., 2015. Dental plaque as a biofilm – a risk in oral cavity and methods to prevent. Postepy Higieny I Medycyny Doswiadczalnej 69: 1140-1148.
'Dental plaque as a biofilm – a risk in oral cavity and methods to prevent ' () 69 Postepy Higieny I Medycyny Doswiadczalnej : 1140 -1148.
Dimidi, E., Christodouildes, S., Fragkos, K.C., Scott, S.M. and Whelan, K., 2014. The effect of probiotics on functional constipation in adults: a systematic review and meta-analysis of randomized controlled trials. American Journal of Clinical Nutrition 100: 1075-1084.
'The effect of probiotics on functional constipation in adults: a systematic review and meta-analysis of randomized controlled trials ' () 100 American Journal of Clinical Nutrition : 1075 -1084.
Hori, T., Kiyoshima, J., Shida, K. and Yasui, J., 2001. Effect of intranasal administration of Lactobacillus casei Shirota on influenza virus infection of upper respiratory tract in mice. Clinical and Diagnostic Laboratory Immunology 8: 593-597.
'Effect of intranasal administration of Lactobacillus casei Shirota on influenza virus infection of upper respiratory tract in mice ' () 8 Clinical and Diagnostic Laboratory Immunology : 593 -597.
How, K.Y., Song, K.P. and Chan, K.G., 2016. Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Frontiers in Microbiology 7: 53.
'Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line ' () 7 Frontiers in Microbiology : 53.
Imamura, T., 2003. The role of gingipains in the pathogenesis of periodontal disease. Journal of Periodontology 74: 111-118.
'The role of gingipains in the pathogenesis of periodontal disease ' () 74 Journal of Periodontology : 111 -118.
Ishihara, K., Nabuchi, A., Ito, R., Miyachi, K., Kuramitsu, H.K. and Okuda, K., 2004. Correlation between detection rates of periodontopathic bacterial DNA in carotid coronary stenotic artery plaque and in dental plaque samples. Journal of Clinical Microbiology 42: 1313-1315.
'Correlation between detection rates of periodontopathic bacterial DNA in carotid coronary stenotic artery plaque and in dental plaque samples ' () 42 Journal of Clinical Microbiology : 1313 -1315.
Iwamoto, Y., Nishimura, F., Nakagawa, M., Sugimoto, H., Shikata, K., Makino, H., Fukuda, T., Tsuji, T., Iwamoto, M. and Murayama, Y., 2001. The effect of antimicrobial periodontal treatment on circulating tumor necrosis factor-alpha and glycated hemoglobin level in patients with type 2 diabetes. Journal of Periodontology 72: 774-778.
'The effect of antimicrobial periodontal treatment on circulating tumor necrosis factor-alpha and glycated hemoglobin level in patients with type 2 diabetes ' () 72 Journal of Periodontology : 774 -778.
Kanehira, T., Yamaguchi, T., Asano, K., Morita, M., Maeshima, E., Matsuda, A., Fujii, Y. and Sakamoto, W., 2011. A screening test for capsaicin-stimulated salivary flow using filter paper: a study for diagnosis of hyposalivation with a complaint of dry mouth. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology 112: 73-80.
'A screening test for capsaicin-stimulated salivary flow using filter paper: a study for diagnosis of hyposalivation with a complaint of dry mouth ' () 112 Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology : 73 -80.
Karunasena, E., McMahon, K.W., Kurkure, P.C. and Brashears, M.M., 2014. A comparison of cell mediators and serum cytokines transcript expression between male and female mice infected with Mycobacterium avium subspecies paratuberculosis and/or consuming probiotics. Pathogens and Disease 72: 104-110.
'A comparison of cell mediators and serum cytokines transcript expression between male and female mice infected with Mycobacterium avium subspecies paratuberculosis and/or consuming probiotics ' () 72 Pathogens and Disease : 104 -110.
Kitagawa, N., 2009. The cause and checkup of dry mouth. Dental Medicine Research 29: 57-61.
'The cause and checkup of dry mouth ' () 29 Dental Medicine Research : 57 -61.
Lamont, R.J. and Jenkinson, H.F., 2000. Subgingival colonization by Porphyromonas gingivalis. Oral Microbiology and Immunology 15: 341-349.
'Subgingival colonization by Porphyromonas gingivalis ' () 15 Oral Microbiology and Immunology : 341 -349.
Macpherson, A.J., McCoy, K.D., Johansen, F-E. and Brandtzaeg, P., 2008. The immune geography of IgA induction and function. Immunology 1: 11-22.
'The immune geography of IgA induction and function ' () 1 Immunology : 11 -22.
Matsubara, V.H., Bandara, H.M., Ishikawa, K.H., Mayer, M.P. and Samaranayake, L.P., 2016. The role of probiotic bacteria in managing periodontal disease: a systematic review. Expert Review of Anti-infective Therapy 14: 643-655.
'The role of probiotic bacteria in managing periodontal disease: a systematic review ' () 14 Expert Review of Anti-infective Therapy : 643 -655.
Matsumoto, S., Hara, T., Hori, T., Mitsuyama, K., Nagaoka, M., Tomiyasu, N., Suzuki, A. and Sata, M., 2005. Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells. Clinical and Experimental Immunology 140: 417-426.
'Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells ' () 140 Clinical and Experimental Immunology : 417 -426.
Mitropoulou, G., Nedovic, V., Goyal, A. and Kourkoutas, Y., 2013. Immobilization technologies in probiotic food production. Journal of Nutrition and Metabolism 2013: 716861.
'Immobilization technologies in probiotic food production ' () 2013 Journal of Nutrition and Metabolism : 716861.
Mojon, P., 2002. Oral health and respiratory infection. Journal of the Canadian Dental Association 68: 340-345.
'Oral health and respiratory infection ' () 68 Journal of the Canadian Dental Association : 340 -345.
Nakagaki, H., Sekine, S., Terao, Y., Toe, M., Tanaka, M., Ito, H.O., Kawabata, S., Shizukuishi, S., Fujihashi, K. and Kataoka, K., 2010. Fusobacterium nucleatum envelope protein FomA is immunogenic and binds to the salivary statherin-derived peptide. Infection and Immunity 78: 1185-1192.
'Fusobacterium nucleatum envelope protein FomA is immunogenic and binds to the salivary statherin-derived peptide ' () 78 Infection and Immunity : 1185 -1192.
Nazir, M.A., 2017. Prevalence of periodontal disease, its association with systemic diseases and prevention. International Journal of Health Sciences 11: 72-80.
'Prevalence of periodontal disease, its association with systemic diseases and prevention ' () 11 International Journal of Health Sciences : 72 -80.
Oberoi, S.S., Harish, Y., Hiremath, S. and Puranik, K., 2016. A cross-sectional survey to study the relationship of periodontal disease with cardiovascular disease, respiratory disease, and diabetes mellitus. Journal of Indian Society of Periodontology 20: 446-452.
'A cross-sectional survey to study the relationship of periodontal disease with cardiovascular disease, respiratory disease, and diabetes mellitus ' () 20 Journal of Indian Society of Periodontology : 446 -452.
O-Brien-Simpson, N.M., Veith, P.D., Dashper, S.G. and Reynolds, E.C., 2003. Porphyromonas gingivalis gingipains: the molecular teeth of a microbial vampire. Current Protein and Peptide Science 4: 409-426.
'Porphyromonas gingivalis gingipains: the molecular teeth of a microbial vampire ' () 4 Current Protein and Peptide Science : 409 -426.
Ohno, H., 2016. Intestinal M cells. Journal of Biochemistry 159: 151-160.
'Intestinal M cells ' () 159 Journal of Biochemistry : 151 -160.
O’Leary, T.J., Drake, R.B. and Naylor, J.E., 1972. The plaque control record. Journal of Periodontology 43: 38.
'The plaque control record ' () 43 Journal of Periodontology : 38.
Sato, J., Gomi, K., Makino, T., Kawasaki, F., Yashima, A., Ozawa, T., Maeda, N. and Arai, T., 2011. The evaluation of bacterial flora in progress of peri-implant disease. Australian Dental Journal 56: 201-206.
'The evaluation of bacterial flora in progress of peri-implant disease ' () 56 Australian Dental Journal : 201 -206.
Sharma, M. and Devi, M., 2014. Probiotics: a comprehensive approach toward health foods. Critical Reviews in Food Science and Nutrition 54: 537-552.
'Probiotics: a comprehensive approach toward health foods ' () 54 Critical Reviews in Food Science and Nutrition : 537 -552.
Singh, V.P., Sharma, J., Babu, S., Rizwanulla and Singla, A., 2013. Role of probiotics in health and disease: a review. Journal of the Pakistan Medical Association 63: 253-257.
'Role of probiotics in health and disease: a review ' () 63 Journal of the Pakistan Medical Association : 253 -257.
Tadokoro, K., Yamaguchi, T., Kawamura, K., Shimizu, H., Egashira, T., Minabe, M., Yoshino, T. and Oguchi, H., 2010. Rapid quantification of periodontitis-related bacteria using a novel modification of Invader PLUS technologies. Microbiological Research 165: 43-49.
'Rapid quantification of periodontitis-related bacteria using a novel modification of Invader PLUS technologies ' () 165 Microbiological Research : 43 -49.
Taguchi, C., Arikawa, K., Saitou, M., Uchiyama, T., Watanabe, I., Tobita, K., Kobayashi, R., Kurita-Ochiai, T. and Nasu, I., 2015. Orally ingested Lactobacillus crispatus KT-11 inhibits Porphyromonas gingivalis-infected alveolar bone resorption. International Journal of Oral-Medical Sciences 13: 102-109.
'Orally ingested Lactobacillus crispatus KT-11 inhibits Porphyromonas gingivalis-infected alveolar bone resorption ' () 13 International Journal of Oral-Medical Sciences : 102 -109.
Takahashi, F., Koji, T. and Morita, O., 2005. The usefulness of an oral moisture checking device (Moisture checker for Mucus®). Nihon Hotetsu Shika Gakkai Zasshi 49: 283-289.
'The usefulness of an oral moisture checking device (Moisture checker for Mucus®) ' () 49 Nihon Hotetsu Shika Gakkai Zasshi : 283 -289.
Tamura, M., Shikina, T., Morihana, T., Hayama, M., Kajimoto, O., Sakamoto, A., Kajimoto, Y., Watanabe, O., Nonaka, C., Shida, K. and Nanno, M., 2007. Effects of probiotics on allergic rhinitis induced by Japanese cedar pollen: randomized double-blind, placebo-controlled clinical trial. International Archives of Allergy and Immunology 143: 75-82.
'Effects of probiotics on allergic rhinitis induced by Japanese cedar pollen: randomized double-blind, placebo-controlled clinical trial ' () 143 International Archives of Allergy and Immunology : 75 -82.
Tobita, K., Yanaka, H. and Otani, H., 2009. Heart-treated Lactobacillus crispatus KT strains reduce allergic symptoms in mice. Journal of Agricultural and Food Chemistry 57: 5586-5590.
'Heart-treated Lactobacillus crispatus KT strains reduce allergic symptoms in mice ' () 57 Journal of Agricultural and Food Chemistry : 5586 -5590.
Tobita, K., Yanaka, H. and Otani, H., 2010a. Anti-allergic effects of Lactobacillus crispatus KT-11 strain on ovalbumin-sensitized BALB/c mice. Animal Science Journal 81: 699-705.
'Anti-allergic effects of Lactobacillus crispatus KT-11 strain on ovalbumin-sensitized BALB/c mice ' () 81 Animal Science Journal : 699 -705.
Tobita, K., Yanaka, H. and Otani, H., 2010b. Lactobacillus crispatus KT-11 enhances intestinal immune functions in C3H/HeN mice. Journal of Nutritional Science and Vitaminology 56: 440-444.
'Lactobacillus crispatus KT-11 enhances intestinal immune functions in C3H/HeN mice ' () 56 Journal of Nutritional Science and Vitaminology : 440 -444.
Tsuchida, S., Satoh, M., Takiwaki, M. and Nomura, F., 2017. Ubiquitination in periodontal disease: a review. International Journal of Molecular Sciences 18: E1476.
'Ubiquitination in periodontal disease: a review ' () 18 International Journal of Molecular Sciences : E1476.
Winning, L. and Linden, G.J., 2017. Periodontitis and systemic disease: association or causality? Current Oral Health Reports 4: 1-7.
'Periodontitis and systemic disease: association or causality? ' () 4 Current Oral Health Reports : 1 -7.
Zalewska, A., Zwierz, K., Zółkowski, K. and Gindzieński, A., 2000. Structure and biosynthesis of human salivary mucins. Acta Biochimica Polonica 47: 1067-1079.
'Structure and biosynthesis of human salivary mucins ' () 47 Acta Biochimica Polonica : 1067 -1079.
All Time | Past 365 days | Past 30 Days | |
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Abstract Views | 511 | 494 | 101 |
Full Text Views | 17 | 8 | 1 |
PDF Views & Downloads | 27 | 17 | 2 |
Heat-treated lactic acid bacteria is added to some foods because it is easier to store and transport, and have less interference with other food ingredient compared with living lactic acid bacteria. We have reported that oral administration of heat-treated Lactobacillus crispatus KT-11 strain (KT-11) suppressed the symptoms of periodontal disease in mice orally infected with Porphyromonas gingivalis. However, the preventive effect of KT-11 on periodontal disease in human is unclear. The aim of this randomised double-blind clinical trial was to examine the effects of KT-11 consumption on the oral environment in healthy volunteers. Sixteen healthy volunteers were randomly assigned to two groups (KT-11 or placebo), and they consumed a KT-11 food tablet (1.2×1010 KT-11 cells) or a placebo food tablet (without KT-11) every day for 4 weeks. Clinical parameters including numbers of major periodontopathic bacteria in saliva, plaque score, gum conditions, and oral mucosal fluid level were evaluated at weeks 0 and 4. Amount of change in P. gingivalis numbers decreased in the KT-11 group compared to that in the placebo group at week 4. Female participants in the KT-11 group had decreased plaque scores, reddish tinge, and gingival swelling scores compared to those in the placebo group at week 4. Furthermore, male participants in the KT-11 group demonstrated increased oral mucosa fluid scores. These clinical findings suggest that daily KT-11 intake can prevent periodontal disease through the improvement of oral conditions.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 511 | 494 | 101 |
Full Text Views | 17 | 8 | 1 |
PDF Views & Downloads | 27 | 17 | 2 |