Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
Argyri, A.A., Zoumpopoulou, G., Karatzas, K.-A.G., Tsakalidou, E., Nychas, G.-J.E., Panagou, E.Z. and Tassou, C.C., 2013. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiology 33: 282-291.
'Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests ' () 33 Food Microbiology : 282 -291.
Banna, G.L., Torino, F., Marletta, F., Santagati, M., Salemi, R., Cannarozzo, E., Falzone, L., Ferraù, F. and Libra, M., 2017. Lactobacillus rhamnosus GG: an overview to explore the rationale of its use in cancer. Frontiers in Pharmacology 8: 603.
'Lactobacillus rhamnosus GG: an overview to explore the rationale of its use in cancer ' () 8 Frontiers in Pharmacology : 603.
Del Carmen, S., De LeBlanc, A.M., Miyoshi, A., Rocha, C.S., Azevedo, V. and LeBlanc, J.G., 2011. Potential application of probiotics in the prevention and treatment of inflammatory bowel diseases. Ulcers 2013: 841651.
'Potential application of probiotics in the prevention and treatment of inflammatory bowel diseases ' () 2013 Ulcers : 841651.
Dimitrellou, D., Kandylis, P., Sidira, M., Koutinas, A.A. and Kourkoutas, Y., 2014. Free and immobilized Lactobacillus casei ATCC 393 on whey protein as starter cultures for probiotic Feta-type cheese production. Journal of Dairy Science 97: 4675-4685.
'Free and immobilized Lactobacillus casei ATCC 393 on whey protein as starter cultures for probiotic Feta-type cheese production ' () 97 Journal of Dairy Science : 4675 -4685.
Doulgeraki, A.I., Pramateftaki, P., Argyri, A.A., Nychas, G.-J.E., Tassou, C.C. and Panagou, E.Z., 2013. Molecular characterization of lactic acid bacteria isolated from industrially fermented Greek table olives. LWT – Food Science and Technology 50: 353-356.
'Molecular characterization of lactic acid bacteria isolated from industrially fermented Greek table olives ' () 50 LWT – Food Science and Technology : 353 -356.
Dylag, K., Hubalewska-Mazgaj, M., Surmiak, M., Szmyd, J. and Brzozowski, T., 2014. Probiotics in the mechanism of protection against gut inflammation and therapy of gastrointestinal disorders. Current Pharmaceutical Design 20: 1149-1155.
'Probiotics in the mechanism of protection against gut inflammation and therapy of gastrointestinal disorders ' () 20 Current Pharmaceutical Design : 1149 -1155.
Faghfoori, Z., Gargari, B.P., Gharamaleki, A.S., Bagherpoure, H. and Khosroushahi, A.Y., 2015. Cellular and molecular mechanisms of probiotics effects on colorectal cancer. Journal of Functional Foods 18: 463-472.
'Cellular and molecular mechanisms of probiotics effects on colorectal cancer ' () 18 Journal of Functional Foods : 463 -472.
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: http://tinyurl.com/zdbrkeg.
Feng, J., Liu, P. and Zhao, X., 2015. Screening of immunomodulatory and adhesive Lactobacillus with antagonistic activities against Salmonella from fermented vegetables. World Journal of Microbiology and Biotechnology 31: 1947-1954.
'Screening of immunomodulatory and adhesive Lactobacillus with antagonistic activities against Salmonella from fermented vegetables ' () 31 World Journal of Microbiology and Biotechnology : 1947 -1954.
Goldin, B.R., Gorbach, S.L., Saxelin, M., Barakat, S., Gualtieri, L. and Salminen, S., 1992. Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Digestive Diseases and Sciences 37: 121-128.
'Survival of Lactobacillus species (strain GG) in human gastrointestinal tract ' () 37 Digestive Diseases and Sciences : 121 -128.
Goldstein, E.J.C., Johnson, S.J., Maziade, P.J., Evans, C.T., Sniffen, J.C., Millette, M. and McFarland, L.V., 2017. Probiotics and prevention of Clostridium difficile infection. Anaerobe 45: 114-119.
'Probiotics and prevention of Clostridium difficile infection ' () 45 Anaerobe : 114 -119.
Granato, D., Branco, G., Gomes Cruz, A., De Assis Fonseca Faria, J. and Shah, N., 2010. Probiotic dairy products as functional foods. Comprehensive Reviews in Food Science and Food Safety 9: 455-470.
'Probiotic dairy products as functional foods ' () 9 Comprehensive Reviews in Food Science and Food Safety : 455 -470.
Hata, A.N., Engelman, J.A. and Faber, A.C., 2015. The BCL2 family: key mediators of the apoptotic response to targeted anticancer therapeutics. Cancer Discovery 5: 475-487.
'The BCL2 family: key mediators of the apoptotic response to targeted anticancer therapeutics ' () 5 Cancer Discovery : 475 -487.
Ijiri, M., Fujiya, M., Konishi, H., Tanaka, H., Ueno, N., Kashima, S., Moriichi, K., Sasajima, J., Ikuta, K. and Okumura, T., 2017. Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells. Tumor Biology 39: 1-12.
'Ferrichrome identified from Lactobacillus casei ATCC334 induces apoptosis through its iron-binding site in gastric cancer cells ' () 39 Tumor Biology : 1 -12.
Iyer, C., Kosters, A., Sethi, G., Kunnumakkara, A.B., Aggarwal, B.B. and Versalovic, J., 2008. Probiotic Lactobacillus reuteri promotes TNF-induced apoptosis in human myeloid leukemia-derived cells by modulation of NF-κB and MAPK signalling. Cellular Microbiology 10: 1442-1452.
'Probiotic Lactobacillus reuteri promotes TNF-induced apoptosis in human myeloid leukemia-derived cells by modulation of NF-κB and MAPK signalling ' () 10 Cellular Microbiology : 1442 -1452.
Konishi, H., Fujiya, M., Tanaka, H., Ueno, N., Moriichi, K., Sasajima, J., Ikuta, K., Akutsu, H., Tanabe, H. and Kohgo, Y., 2016. Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis. Nature Communication 7: 12365.
'Probiotic-derived ferrichrome inhibits colon cancer progression via JNK-mediated apoptosis ' () 7 Nature Communication : 12365.
Lebeer, S., Claes, I., Tytgat, H.L., Verhoeven, T.L., Marien, E., Von Ossowski, I., Reunanen, J., Palva, A., Vos, W.M., Keersmaecker, S.C. and Vanderleyden, J., 2012. Functional analysis of Lactobacillus rhamnosus GG pili in relation to adhesion and immunomodulatory interactions with intestinal epithelial cells. Applied Environmental Microbiology 78: 185-193.
'Functional analysis of Lactobacillus rhamnosus GG pili in relation to adhesion and immunomodulatory interactions with intestinal epithelial cells ' () 78 Applied Environmental Microbiology : 185 -193.
Mantegazza, C., Molinari, P., D’Auria, E., Sonnino, M., Morelli, L. and Zuccotti, G.V., 2017. Probiotics and antibiotic-associated diarrhea in children: a review and new evidence on Lactobacillus rhamnosus GG during and after antibiotic treatment. Pharmacological Research 128: 63-72.
'Probiotics and antibiotic-associated diarrhea in children: a review and new evidence on Lactobacillus rhamnosus GG during and after antibiotic treatment ' () 128 Pharmacological Research : 63 -72.
Orlando, A., Refolo, M.G., Messa, C., Amati, L., Lavermicocca, P., Guerra, V. and Russo, F., 2012. Antiproliferative and proapoptotic effects of viable or heat-killed Lactobacillus paracasei IMPC2.1 and Lactobacillus rhamnosus GG in HGC-27 gastric and DLD-1 colon cell lines. Nutrition and Cancer 64: 1103-1111.
'Antiproliferative and proapoptotic effects of viable or heat-killed Lactobacillus paracasei IMPC2.1 and Lactobacillus rhamnosus GG in HGC-27 gastric and DLD-1 colon cell lines ' () 64 Nutrition and Cancer : 1103 -1111.
Österlund, P., Ruotsalainen, T., Korpela, R., Saxelin, M., Ollus, A., Valta, P., Kouri, M., Elomaa, I. and Joensuu, H., 2007. Lactobacillus supplementation for diarrhoea related to chemotherapy of colorectal cancer: a randomised study. British Journal of Cancer 97: 1028-1034.
'Lactobacillus supplementation for diarrhoea related to chemotherapy of colorectal cancer: a randomised study ' () 97 British Journal of Cancer : 1028 -1034.
Ouwehand, A.C. and Salminen, S., 2003. In vitro adhesion assays for probiotics and their in vivo relevance: a review. Microbial Ecology in Health and Disease 15: 175-184.
'In vitro adhesion assays for probiotics and their in vivo relevance: a review ' () 15 Microbial Ecology in Health and Disease : 175 -184.
Pace, F., Pace, M. and Quartarone, G., 2015. Probiotics in digestive diseases: focus on Lactobacillus GG. Minerva Gastroenterologica e Dietologica 61: 273-292.
'Probiotics in digestive diseases: focus on Lactobacillus GG ' () 61 Minerva Gastroenterologica e Dietologica : 273 -292.
Petrova, M.I., Imholz, N.C., Verhoeven, T.L., Balzarini, J., Van Damme, E.J., Schols, D., Vanderleyden, J. and Lebeer, S., 2016. Lectin-like molecules of Lactobacillus rhamnosus GG inhibit pathogenic Escherichia coli and Salmonella biofilm formation. PLoS ONE 18: e0161337.
'Lectin-like molecules of Lactobacillus rhamnosus GG inhibit pathogenic Escherichia coli and Salmonella biofilm formation ' () 18 PLoS ONE : e0161337.
Plessas, S., Nouska, C., Karapetsas, A., Kazakos, S., Alexopoulos, A., Mantzourani, I., Chondrou, P., Fournomiti, M., Galanis, A. and Bezirtzoglou, E., 2017. Isolation, characterization and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from Feta-type cheese. Food Chemistry 226: 102-108.
'Isolation, characterization and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from Feta-type cheese ' () 226 Food Chemistry : 102 -108.
Saxami, G., Karapetsas, A., Lamprianidou, E., Kotsianidis, I., Chlichlia, A., Tassou, C.C., Zoumpourlis, V. and Galanis, A., 2016. Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines. Journal of Functional Foods 24: 461-471.
'Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines ' () 24 Journal of Functional Foods : 461 -471.
Saxami, G., Ypsilantis, P., Sidira, M., Simopoulos, C., Kourkoutas, Y. and Galanis, A., 2012. Distinct adhesion of probiotic strain Lactobacillus casei ATCC 393 to rat intestinal mucosa. Anaerobe 18: 417-420.
'Distinct adhesion of probiotic strain Lactobacillus casei ATCC 393 to rat intestinal mucosa ' () 18 Anaerobe : 417 -420.
Schlee, M., Wehkamp, J., Altenhoefer, A., Oelschlaeger, T.A., Stange, E.F. and Fellermann, K., 2007. Infection and Immunity 75: 2399-2407.
() 75 Infection and Immunity : 2399 -2407.
Sidira, M., Galanis, A., Nikolaou, A., Kanellaki, M. and Kourkoutas, Y., 2014a. Evaluation of Lactobacillus casei ATCC 393 protective effect against spoilage of probiotic dry fermented sausages. Food Control 42: 315-320.
'Evaluation of Lactobacillus casei ATCC 393 protective effect against spoilage of probiotic dry fermented sausages ' () 42 Food Control : 315 -320.
Sidira, M., Galanis, A., Ypsilantis, P., Karapetsas, A., Progaki, Z., Simopoulos, C. and Kourkoutas, Y., 2010. Effect of probiotic-fermented milk administration on gastrointestinal survival of Lactobacillus casei ATCC 393 and modulation of intestinal microbial flora. Journal of Molecular Microbiology and Biotechnology 19: 224-230.
'Effect of probiotic-fermented milk administration on gastrointestinal survival of Lactobacillus casei ATCC 393 and modulation of intestinal microbial flora ' () 19 Journal of Molecular Microbiology and Biotechnology : 224 -230.
Sidira, M., Karapetsas, A., Galanis, A., Kanellaki, M. and Kourkoutas, Y., 2014b. Effective survival of immobilized Lactobacillus casei during ripening and heat treatment of probiotic dry-fermented sausages and investigation of the microbial dynamics. Meat Science 96: 948-955.
'Effective survival of immobilized Lactobacillus casei during ripening and heat treatment of probiotic dry-fermented sausages and investigation of the microbial dynamics ' () 96 Meat Science : 948 -955.
Sidira, M., Kourkoutas, Y., Kanellaki, M. and Charalampopoulos, D., 2015. In vitro study on the cell adhesion ability of immobilized lactobacilli on natural supports. Food Research International 76: 532-539.
'In vitro study on the cell adhesion ability of immobilized lactobacilli on natural supports ' () 76 Food Research International : 532 -539.
Sidira, M., Saxami, G., Dimitrellou, D., Santarmaki, V., Galanis, A. and Kourkoutas, Y., 2013. Monitoring survival of Lactobacillus casei ATCC 393 in probiotic yogurts using an efficient molecular tool. Journal of Dairy Science 96: 1-9.
'Monitoring survival of Lactobacillus casei ATCC 393 in probiotic yogurts using an efficient molecular tool ' () 96 Journal of Dairy Science : 1 -9.
Terpou, A., Bosnea, L., Kanellaki, M., Bekatorou, A., Plessas, S., Bezirtzoglou, E. and Koutinas A., 2017. Growth capacity of the novel Lactobacillus paracasei K5 strain incorporated in industrial white brine cheese as an adjunct. Journal of Food Science 83: 723-731.
'Growth capacity of the novel Lactobacillus paracasei K5 strain incorporated in industrial white brine cheese as an adjunct ' () 83 Journal of Food Science : 723 -731.
Thirabunyanon, M. and Hongwittayakorn, P., 2013. Potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction. Applied Biochemistry and Biotechnology 169: 511-525.
'Potential probiotic lactic acid bacteria of human origin induce antiproliferation of colon cancer cells via synergic actions in adhesion to cancer cells and short-chain fatty acid bioproduction ' () 169 Applied Biochemistry and Biotechnology : 511 -525.
Tiptiri-Kourpeti, A., Spyridopoulou, K., Santarmaki, V., Aindelis, G., Tompoulidou, E., Lamprianidou, E.E., Saxami, G., Ypsilantis, P., Lampri, E.S., Simopoulos, C., Kotsianidis, I., Galanis, A., Kourkoutas, Y., Dimitrellou, D. and Chlichlia, K., 2016. Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells. PLoS ONE 11: e0147960.
'Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells ' () 11 PLoS ONE : e0147960.
Uccello, M., Malaguarnera, G., Basile, F., D’agata, V., Malaguarnera, M., Bertino, G., Vacante, M., Drago, F. and Biondi, A., 2012. Potential role of probiotics on colorectal cancer prevention. BMC Surgery 12, Suppl. 1: S35.
'Potential role of probiotics on colorectal cancer prevention ' () 1 : S35.
Valeur, N., Engel, P., Carbajal, N., Connolly, E. and Ladefoged, K., 2004. Colonization and immunomodulation by Lactobacillus reuteri ATCC 55730 in the human gastrointestinal tract. Applied Environmental Microbiology 70: 1176-1181.
'Colonization and immunomodulation by Lactobacillus reuteri ATCC 55730 in the human gastrointestinal tract ' () 70 Applied Environmental Microbiology : 1176 -1181.
Wang, G., Zhao, Y., Tian, F., Jin, X., Chen, H., Liu, X., Zhang, Q., Zhao, J., Chen, Y., Zhang, H. and Chen, W., 2014. Screening of adhesive lactobacilli with antagonistic activity against Campylobacter jejuni. Food Control 44: 49-57.
'Screening of adhesive lactobacilli with antagonistic activity against Campylobacter jejuni ' () 44 Food Control : 49 -57.
Wang, Y.H., Yao, N., Wei, K.K., Jiang, L., Hanif, S., Wang, Z.X. and Pei, C.X., 2016. The efficacy and safety of probiotics for prevention of chemoradiotherapy-induced diarrhea in people with abdominal and pelvic cancer: a systematic review and meta-analysis. European Journal of Clinical Nutrition 70: 1246-1253.
'The efficacy and safety of probiotics for prevention of chemoradiotherapy-induced diarrhea in people with abdominal and pelvic cancer: a systematic review and meta-analysis ' () 70 European Journal of Clinical Nutrition : 1246 -1253.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 383 | 331 | 107 |
Full Text Views | 16 | 10 | 0 |
PDF Views & Downloads | 26 | 10 | 0 |
Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 383 | 331 | 107 |
Full Text Views | 16 | 10 | 0 |
PDF Views & Downloads | 26 | 10 | 0 |