Effect of dietary protein level and salinity on growth, survival, enzymatic activities and amino-acid composition of the white shrimp Litopenaeus vannamei (Boone, 1931) juveniles

in Crustaceana
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Litopenaeus vannamei (Boone, 1931) postlarvae with average initial body weight of 0.089 g were reared in 75-litre PVC tanks for 40 days at salinities of 30 and 60 g l−1. The shrimps were fed compound feed containing protein levels of 35, 40, 45 and 50%, respectively. Salinity had a remarkable effect on growth and survival of L. vannamei juveniles. Higher survival rate and lower growth were observed at 60 g l−1 salinity. Dietary protein level affected the survival and growth of juveniles at both salinities, increased with dietary protein levels in the range of 35 to 45%, but decreased slightly with 50% dietary protein. Broken line analysis showed that the estimated optimal dietary protein levels at salinities of 30 and 60 g l−1 were 45.93 and 46.74%, respectively. Higher salinity resulted in an increased moisture content, ash and crude protein content in the shrimp muscle tissue. The amino acid contents in the shrimp muscle tissue were generally higher at 60 g l−1 salinity and increased dietary protein level led to higher protein content, except with 50% dietary protein. At salinity 60 g l−1, the soluble protein content and activities of glutamic oxalacetic transferase (GOT) and glutamic pyruvic transaminase (GPT) in shrimp muscle tissue were higher, while catalase (CAT) activities were lower. Farming of L. vannamei at a marginal culture salinity (60 g l−1) is feasible though the shrimps were likely exposed to stressful conditions. Reduced growth rate at higher salinity may be attributed to the higher total ammonium (TAN) concentration in the culture medium and extra energy consumption for osmoregulation at hypersaline conditions.

Effect of dietary protein level and salinity on growth, survival, enzymatic activities and amino-acid composition of the white shrimp Litopenaeus vannamei (Boone, 1931) juveniles

in Crustaceana

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References

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Figures

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    Survival of Litopenaeus vannamei (Boone, 1931) fed compound feeds with various dietary protein levels at 30 g l−1 and 60 g l−1 salinity. The bar represents mean value of four replicates with standard deviation. Lower-case and upper-case letters represent statistically significant differences at 30 g l−1 and 60 g l−1 salinity, respectively (P<0.05).

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    Specific growth rate of Litopenaeus vannamei (Boone, 1931) fed compound feeds with various dietary protein levels at 30 g l−1 and 60 g l−1 salinity. The bar represents mean value of four replicates with standard deviation. Lower-case and upper-case letters represent statistically significant differences at 30 g l−1 and 60 g l−1 salinity, respectively (P<0.05).

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    Estimated optimum dietary protein levels Litopenaeus vannamei (Boone, 1931) reared at (A) 30 g l−1 and (B) 60 g l−1 salinity by broken line analysis.

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    Total ammonium concentration in culture medium of Litopenaeus vannamei (Boone, 1931) fed compound feeds with various dietary protein levels at 30 g l−1 and 60 g l−1 salinity.

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