The aim of this study was to determine the acute toxicity effect and the accumulation of microcystin-leucine-arginine (MC-LR) on the crayfish Procambarus clarkii (Girard, 1852). Juvenile P. clarkii (5.47 ± 1.3 g) were cultured under 5 different MC-LR concentrations (0.3, 0.6, 1.2, 2.4, 4.8 mg/l), then the acute toxicity effect was observed. The median lethal concentration (LC50) of MC-LR on juvenile P. clarkii was 3.741 mg/l at 24 h, 1.494 mg/l at 48 h, 0.817 mg/l at 72 h and 0.567 mg/l at 96 h. Accumulation of MC-LR was measured in different organs of mature P. clarkii (58.7 ± 3.8 g) exposed to 0.3 mg/l MC-LR for 120 h. The detected MC-LR concentration decreased in the sequence: hepatopancreas > ovary > abdominal muscle > intestine. Hepatopancreas and ovary were found to be the main targets of the toxin. The results suggested that the MC-LR produced by cyanobacteria blooms could not only reduce the survival rate of juvenile P. clarkii but also affect the fecundity of mature crayfish. This research also provides a reference basis for the detection and assessment of the pollution of water bodies in P. clarkii culture.
CazenaveJ.BistoniM. A.PesceS. F.WunderlinD. A., 2006.
Differential detoxification and antioxidant response in diverse organs of Corydoras paleatus experimentally exposed to microcystin-RR.
LindsayJ.MetcalfJ. S.CoddG. A., 2006.
Protection against the toxicity of microcystin-LR and cylindrospermopsin in Artemia salina and Daphnia spp. by pretreatment with cyanobacterial lipopolysacchari (LPS).
MarceloG. G.IlieS. R.HunbertoV., 2003.
Variation in lipid, protein, and carbohydrate content during the embryonic development of the crayfish Cherax quadricarinatus (Decapoda: Parastacidae).
J. Crustacean Biol.,
Depuration of microcystin-LR from the red swamp crayfish P. clarkii with assessment of its food quality.