Scanning electron microscopy (SEM) is widely used in studies on crustacean systematics and functional morphology. The surface ultrastructure of cladoceran ephippia may carry valuable information for taxonomy, and its analysis may be also helpful in palaeoecological studies focusing on ephippia preserved in old sediments. SEM is also commonly used in analyses of cladoceran filtering limbs, which not only serve for filtering of food particles, but are also taxonomically important structures. In this study, we describe an efficient method for preparing both ephippia and limbs for SEM analyses. The workflow minimizes physical manipulation, which may reduce the risk of damage or loss of material, and allows a relatively large amount of material to be studied. We also evaluated the effects of two strong chemical agents used to remove unwanted biofilm from both ephippia and limb surfaces. This approach may further facilitate SEM analyses in systematic, ecological and palaeoecological surveys of Cladocera.
La microcopie électronique à balayage (MEB) est largement utilisée dans les études sur la systématique et la morphologie fonctionnelle des crustacés. L’ultrastructure de la surface des éphippies de cladocères peut apporter des informations précieuses pour la taxonomie, et son analyse peut aussi être utile lors dans les études paléoécologiques des éphippies conservées dans les sédiments anciens. La MEB est aussi couramment utilisée dans l’analyse des appendices filtreurs des cladocères, qui ne servent pas seulement à la filtration des particules alimentaires, mais sont aussi des structures taxonomiquement importantes. Dans cette étude, nous décrivons une méthode efficace de préparation à la fois des éphippies et des appendices pour les analyses en MEB. Le processus utilisé minimise la manipulation physique, ce qui peut réduire le risque de dommage ou de perte de matériel, et permet l’étude d’une relative grande quantité de matériel. Nous avons aussi évalué les effets de deux puissants agents chimiques utilisés pour retirer le biofilm indésirable à la surface à la fois des éphippies et des appendices. Cette approche pourra ultérieurement faciliter les analyses en MEB pour l’étude systématique, écologique et paléoécologique des Cladocera.
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Scanning electron microscopy (SEM) is widely used in studies on crustacean systematics and functional morphology. The surface ultrastructure of cladoceran ephippia may carry valuable information for taxonomy, and its analysis may be also helpful in palaeoecological studies focusing on ephippia preserved in old sediments. SEM is also commonly used in analyses of cladoceran filtering limbs, which not only serve for filtering of food particles, but are also taxonomically important structures. In this study, we describe an efficient method for preparing both ephippia and limbs for SEM analyses. The workflow minimizes physical manipulation, which may reduce the risk of damage or loss of material, and allows a relatively large amount of material to be studied. We also evaluated the effects of two strong chemical agents used to remove unwanted biofilm from both ephippia and limb surfaces. This approach may further facilitate SEM analyses in systematic, ecological and palaeoecological surveys of Cladocera.
La microcopie électronique à balayage (MEB) est largement utilisée dans les études sur la systématique et la morphologie fonctionnelle des crustacés. L’ultrastructure de la surface des éphippies de cladocères peut apporter des informations précieuses pour la taxonomie, et son analyse peut aussi être utile lors dans les études paléoécologiques des éphippies conservées dans les sédiments anciens. La MEB est aussi couramment utilisée dans l’analyse des appendices filtreurs des cladocères, qui ne servent pas seulement à la filtration des particules alimentaires, mais sont aussi des structures taxonomiquement importantes. Dans cette étude, nous décrivons une méthode efficace de préparation à la fois des éphippies et des appendices pour les analyses en MEB. Le processus utilisé minimise la manipulation physique, ce qui peut réduire le risque de dommage ou de perte de matériel, et permet l’étude d’une relative grande quantité de matériel. Nous avons aussi évalué les effets de deux puissants agents chimiques utilisés pour retirer le biofilm indésirable à la surface à la fois des éphippies et des appendices. Cette approche pourra ultérieurement faciliter les analyses en MEB pour l’étude systématique, écologique et paléoécologique des Cladocera.
All Time | Past 365 days | Past 30 Days | |
---|---|---|---|
Abstract Views | 538 | 74 | 7 |
Full Text Views | 182 | 1 | 0 |
PDF Views & Downloads | 28 | 2 | 0 |