A colour variation of Clibanarius virescens (Krauss, 1843) (Decapoda, Anomura) collected from Amami Oshima Island and Okinawa, Japan

in Crustaceana
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During our survey on the intertidal hermit crab fauna on Amami Oshima Island and Okinawa Island, southwestern Japan, we collected specimens identified as Clibanarius species by their morphological features, but that had an unknown coloration for the genus: they have whitish ambulatory legs and a whitish shield. For species identification of these specimens, we observed their coloration and morphology and also performed phylogenetic analyses using the genes mitochondrial cytochrome c oxidase I (COI) and nuclear histone H3. The analysis has shown that these specimens are to be identified as Clibanarius virescens (Krauss, 1843).

A colour variation of Clibanarius virescens (Krauss, 1843) (Decapoda, Anomura) collected from Amami Oshima Island and Okinawa, Japan

in Crustaceana



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    Map showing the study sites: Amami Oshima Island and Okinawa Island: A, Tatsugo-chou, Amami Oshima Island, Kagoshima; B, Gushikawa, Uruma-shi, Okinawa.

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    Comparison with normal colour and WL of Clibanarius virescens (Krauss, 1843): a, SMBL-AY 206, male, normal colour individual collected in Shirahama, Wakayama; b, SMBL-AY454, male colour variant collected in Tatsugo-chou, Amami Oshima, Kagoshima; c-d, SMBL-AY523, male and SMBL-AY527 female, colour variants collected in Gushikawa, Uruma-shi, Okinawa.

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    Colour pattern of chelipeds and right second and third pereopods, WL individual, SMBL-AY 454. Chelipeds: a, dorsal; b, ventral; c, lateral; d, mesial. Second pereopod (right): e, lateral; f, mesial. Third pereopod (right): g, lateral; h, mesial.

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    Maximum likelihood tree based on COI data (510 bp) with bootstrap values (based on 1000 pseudoreplications). An asterisk indicates WL individuals. Calcinus laevimanus (Randall, 1840), Coenobita perlatus (Milne Edwards, 1853) and Dardanus sanguinocarpus Degener, 1925 are used as outgroups. A, B and C indicate three colour types of Clibanarius virescens (Krauss, 1843) according to Morgan (1985). See text for an explanation of types A-C.

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    Maximum likelihood tree based on histone 3 data (304 bp) with the morphologically similar sympatric species with bootstrap values (based on 1000 pseudoreplications). A-C as in fig. 4.

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    Colour variations of Clibanarius virescens (Krauss, 1843): I, SMBL-AY1296 (Female, SL = 2.9 mm), normal colour individual collected in Shirahama, Wakayama; II, SMBL-AY454 (Male, SL = 2.6 mm), one of the WL individual collected in Tatsugo-chou, Amami Oshima, Kagoshima.

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