Morphological and genetic characterization of a new Armadillidium species (Oniscidea, Armadillidiidae) from Tunisia

in Crustaceana
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Armadillidium boukorninense n. sp., a new species collected in northeastern Tunisia, is described. This species can be easily recognised by the tegument structure, the morphology of the cephalon and the high differentiation of the male pereopod 7. Its geographical distribution is limited to the northeast of Tunisia, in the maquis and garrigues habitats of sub-humid and semi-arid areas. This new species is morphologically similar to A. decorum. However, the morphological comparison between these two species showed a divergence at the cephalon, hind margin of pereon-epimeron 1, pereopod VII and pleotelson. An allozyme analysis of ten enzymatic systems (ME, PHI, EST, MPI, ACPH, GOT, IDH, PEP, PGM, MDH) confirmed this morphological differentiation. Among the eighteen loci that were analysed, differentiation in six diagnostic (Mpi, Pgm, Me, Acph-2, Pep-3, Got-2) and two partially-diagnostic loci (Idh-1, Phi),were easily recognized. Furthermore, high values of Nei’s genetic distance (Nei, 1978) (D¯=0.69±0.1) and FST=0.72 indicated a high genetic differentiation of these two species. When taking into account these two approaches, A. boukorninense n. sp. is morphologically and genetically different from A. decorum.

Morphological and genetic characterization of a new Armadillidium species (Oniscidea, Armadillidiidae) from Tunisia

in Crustaceana



AyalaF. J.PowelJ. R.TraceyM. L.MouraoC. A.Perez-SalasS.1972. Enzyme variability in the Drosophila willistoni group. IV. Genic variation in natural populations of Drosophila willistoni. Genetics70: 113-139.

BattiatoC.1995. Differenziamento genetico e morfologico di alcune specie epigee e cavernicole di Armadillidium di Sicilia e di Malta (Crustacea Isopoda Oniscidea). (Ph.D. Thesis University of Catania Catania).

BrewerG. J.1970. An introduction to isozyme techniques. (Academic PressNew York).

CarusoD.BaglieriC.Di MaioM. C.LombardoB. M.1987. Isopodi terrestri di Sicilia ed Isole circumsiciliane. Animalia14: 5-211.

Hamaied-MelkiS.2008. Systématique distribution géographique et écologie du genre Armadillidium en Tunisie (Crustacés Isopodes Oniscidea). (Ph.D. Thesis University of Tunis El Manar Tunis).

HamaiedS.Charfi-CheikhrouhaF.2007. Description d’une nouvelle espèce du genre Armadillidium (Isopoda, Oniscidea) de Tunisie. Crustaceana80: 523-532.

HamaiedS.Charfi-CheikhrouhaF.2017. Description d’une nouvelle espèce de Armadillidium Brandt, 1833 (Isopoda, Oniscidea) de Tunisie. Crustaceana90: 1463-1471.

HarrisH.HopkinsonD. A.1976. Handbook of enzyme electrophoresis in human genetics. (American ElsevierNew York).

La GrecaM.1989. La Zoogeografia e la tettonica a placche. Scienza e tecnica Ann. EST89/90: 122-134.

LaulierM.1988. Génétiques et systématique évolutives du complexe d’espèces Sphaeroma hookeri Leach, Sphaeroma levii Argano, et Sphaeroma rugicauda Leach (Crustacés, Isopodes, Flabellifères). 1. Génétique formelle de onze locus enzymatiques. Genetics Selection Evolution20(1): 63-74.

LombardoB. M.ViglianisiF. M.2002. Differenziamento genetico tra due specie troglobie di Sicilia e di Malta: Armadillidium lagrecai Vandel 1969 e Armadillidium ghardalamensis Caruso e Hili 1991 (Crustacea Isopoda Oniscidea). Atti dell’Accademia Gioenia di Scienze Naturali in Catania36: 603-610.

LombardoB. M.ViglianisiF. M.CarusoD.2001. Definizione, su base genetica e morfologica, di alcune specie di Armadillidium di Sicilia, Calabria, Isole Circumsiciliane e Tunisia (Crustacea, Isopoda, Oniscidea). Naturalista siciliano25: 397-412.

MediniL.2002. Systématique Biologie et Biogéographie du genre Porcellio en Tunisie (Crustacé Isopodes Oniscidea). (Ph.D. Thesis University of Tunis El Manar Tunis).

NeiM.1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics89: 583-590.

PasteurN.PasteurG.BonhommeF.CatalanJ.Britton-DavidianJ.1988. Practical isozyme genetics: 1-215. (Ellis Horwood PublisherChichester).

SelanderR. K.SmithM. H.YangS. Y.JohnsonW. E.GentryJ. B.1971. Biochemical polymorphism and systematics in the genus Peromyscus. IV. Variation in the old-field mouse (Peromyscus polionotus). Studies in Genetics6: 49-90.

SweeneyB. W.FunkD. H.VannoteR. L.1987. Genetic variation in stream mayfly (Insecta: Ephemeroptera) populations of eastern North America. Annals of the Entomological Society of America80: 600-612.

SwoffordD. L.SelanderB.1981. A computer program for the analysis of allelic variation in genetics. Journal of Heredity72: 281-283.

VandelA.1969. Les Isopodes terrestres de la Sicile. Atti dell’Accademia Gioenia di Scienze naturali in Catania Serie 71: 1-59.

ViglianisiF.LombardoB. M.CarusoD.1992. Differenziamento genetico e morfologico in alcune specie siciliane di Isopodi terrestri del genere Porcellio e descrizione di tre nuove specie (Crustacea, Isopoda, Oniscoïdea). Animalia19: 235-273.

WrightS.1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution19: 395-420.


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    Distribution of Armadillidium boukorninense n. sp. and geographic origin of the examined populations of this new species and A. decorum Brandt, 1833. Abbreviations: BO, Boukornine; CO, Collesano; DA, Dar Allouch; EP, Epipoli; JE, Jabbès; MB, between Menzel Bouzelfa and Menzel Temime; ME, Mellili; NA, Nahli; PA, Palazzo Adriano; PT, Porto di Tindari.

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    Armadillidium boukorninense n. sp. A, male side view; B, cephalon (side view); C, cephalon (dorsal view); D, cephalon (frontal view); E, pleotelson.

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    Armadillidium boukorninense n. sp. A, pereopod 1 male (sternal view); B, pereopod 1 male (tergal view); C, pereopod 7 male (sternal view); E, pereopod 7 male (tergal view).

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    Armadillidium boukorninense n. sp. A, antenna; B, antennula; C, exopodite of pleopod 1 male; D, endopodite of pleopod 1 male; E, pleopod 2 male; F, uropod.

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    Armadillidium decorum Brandt, 1833. A, flagellum of antenna; B, cephalon (dorsal view); C, cephalon (side view); D, cephalon (ventral view); E, pereopod 7 male (sternal view); F, pleotelson; G, exopodite of pleopod I.

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    UPGMA dendrogram based on Nei’s genetic distance among the three populations belonging to two species of Armadillidium from Sicily and Tunisia.

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