Restraining small decapods and amphipods for in vivo laboratory studies
In: CrustaceanaBaikal Research Centre, Lenin st. 21, Irkutsk 664003, Russia
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Institute of Biologyat Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya st. 11, Petrozavodsk 185035, Russia
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Abstract
During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail.
Résumé
Au cours des études physiologiques, il est souvent nécessaire d’immobiliser un petit animal pour une expérience, et il est difficile d’effectuer des mesures ou de l’imagerie in vivo sur des individus libres et mouvants. Dans cet article nous décrivons deux approches, qui sont bien établies dans notre laboratoire, applicables largement pour une immobilisation répétée de petits (∼0.5-4 cm) décapodes et amphipodes : immobilisation par collage doux et aspiration. L’application des ces deux approches ainsi que leurs avantages et désavantages sont discutés en détail.
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| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 497 | 109 | 19 |
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Restraining small decapods and amphipods for in vivo laboratory studies
In: CrustaceanaBaikal Research Centre, Lenin st. 21, Irkutsk 664003, Russia
Search for other papers by Anton Gurkov in
Current site
Google Scholar
PubMed
Institute of Biologyat Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya st. 11, Petrozavodsk 185035, Russia
Search for other papers by Ekaterina Borvinskaya in
Current site
Google Scholar
PubMed
Search for other papers by Ekaterina Shchapova in
Current site
Google Scholar
PubMed
Search for other papers by Maxim Timofeyev in
Current site
Google Scholar
PubMed
Abstract
During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 497 | 109 | 19 |
| Full Text Views | 189 | 2 | 0 |
| PDF Views & Downloads | 23 | 4 | 0 |