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Restraining small decapods and amphipods for in vivo laboratory studies

In: Crustaceana
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  • 1 Institute of Biologyat Irkutsk State University, Lenin st. 3, Irkutsk 664025, Russia
  • | 2 Baikal Research Centre, Lenin st. 21, Irkutsk 664003, Russia
  • | 3 Institute of Biologyat Karelian Research Centre of Russian Academy of Sciences, Pushkinskaya st. 11, Petrozavodsk 185035, Russia
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Abstract

During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail.

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