Characterization of a protandrous hermaphroditic reproductive system in transitional and female phases of Norileca indica — morphological, histological and ultrastructural approach

in Crustaceana
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Abstract

The present study explores the reproductive system of Norileca indica during its transitional and female phase at morphological, histological, ultrastructural and histochemical levels. The paired and symmetrical hermaphroditic reproductive system of N. indica in the transitional and female phases lies dorsally in the thorax on either side of the gut, each consisting of a three-lobed testis (with lobes t1, t2 and t3) followed by an ovary and then a vas deferens, which opens into the paired penes located at sternite 7; the oviduct, arising laterally from the ovarian lobe, opens into the gonopore located on the 6th pereonite. In the transitional phase, the gonads show a presence of germ cells at different maturation stages: spermiogenesis in the testes has already halted, while the ovary undergoes active vitellogenesis. Spermatophores are frequently seen in the vas deferens but seldom in the testes; the size of the oocytes then is 250-1200 μm. The gonadosomatic index (GSI) and nucleocytoplasmic index (NCI) range over 0.090-0.198 and 0.46-0.11, respectively. In the female, oocyte size increases to 1500 μm; the GSI ranges 0.019-0.235 and the NCI from 2.40 to 0.09; testes and vas deferens are not prominent. This paper discusses the possible role of protandrous hermaphroditism in the reproductive life of N. indica.

Characterization of a protandrous hermaphroditic reproductive system in transitional and female phases of Norileca indica — morphological, histological and ultrastructural approach

in Crustaceana

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References

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Figures

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    Norileca indica (H. Milne Edwards, 1840) and its reproductive system: A, transitional stage; B, female stage; C, penes in transitional specimen; D, brood plate in female.

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    Characteristics of the ovary in the transitional and female phases of Norileca indica (H. Milne Edwards, 1840)

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    Position in situ (lefthand pictures) and detailed morphological features (righthand photo’s) of the ovary with respect to different maturation stages in the transitional and female phases of Norileca indica (H. Milne Edwards, 1840) (viewed at 10× magnification): A-B, PVS; C-D, Vg-1; E-F, Vg-2; G-H, Vg-3; I-J, S. Legends: PVS, previtellogenic stage; Vg-1, 2, 3, vitellogenic stages 1, 2, and 3, respectively; S, spent stage; g, gonad; t, testis lobes; o, ovary; p1-p7, pereonites 1-7; od, oviduct; vd, vas deferens; Oc, oocyte.

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    Hermaphroditic gonad and the various ducts in Norileca indica (H. Milne Edwards, 1840) in the transitional phase: a, gonad in early transitional stage; b, gonad in late transitional stage; A, testis in early transitional stage (1000×); B, testis in late transitional phase (1000×); C, ovary showing yolky oocytes (200×); D, ovary showing follicle cells (semithin LS: 1 μm; methylene blue; 1000×); E, vas deferens (1000×); F, oviduct (LS: 4 μm; Haematoxylin-Eosin; 1000×). Legends: LS, longitudinal section; Oo, oocytes; sp, spermatophore; fc, follicle cell; t, tails of spermatozoa; n, nuclei of spermatophore.

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    Histology of the testis, vas deferens and oviduct in the female phase of Norileca indica (H. Milne Edwards, 1840): A, testis; B, vas deferens without spermatophores; C, vas deferens showing spermatophores; D, oviduct without spermatophores; E, oviduct showing spermatophores. Legends: Od-1, -2, -3, respectively, epithelial cell types 1, 2, and 3; s, spermatophore; st, sperm tail; t1, 2, 3, respectively, testis lobes 1, 2, and 3.

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    Histology of the ovary at different stages in the female phase of Norileca indica (H. Milne Edwards, 1840): A, PVS (200×); B, Vg-1 (400×); C, Vg-2 (200×); D, Vg-3 (200×); E, early S (200×); F, late S (200×). LS: 4 μm, Haematoxylin-Eosin. Legends: gz, germinal zone; n, nucleus; pvOo, previtellogenic oocyte; vOo, vitellogenic oocyte; fc 1, follicle cell type 1; fc 2, follicle cell type 2; nl, nucleolus; t, trabeculae; s, sinus.

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    Electron micrographs of previtellogenic ovary in the female phase of Norileca indica (H. Milne Edwards, 1840): A, convoluted ovary wall (1900×); B, follicle cells (11 000×); C, previtellogenic oocyte (9300×). Legends: d, desmosomes; m, mitochondria; n, nucleus; ow, ovary wall.

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    Histochemistry of ovary at different stages in the female phase of Norileca indica (H. Milne Edwards, 1840): A-D, PAS test (400×); A, Vg-1; B, Vg-2; C, Vg-3; D, Spent; E-H, Sudan Black test (400×); E, Vg-1; F, Vg-2; G, Vg-3; H, Spent; I-L, MBB test (400×); I, Vg-1; J, Vg-2; K, Vg-3; L, Spent; M-N, Basic Fuchsin test (200×); M, PVS; N, Vg-1; O, Spent.

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    Correlation between ovarian cycle and marsupial development in Norileca indica (H. Milne Edwards, 1840).

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