Save

Molecular cloning, tissue distribution and gene expression in response to nutritional regulation of sterol regulatory element binding protein-1 from the swimming crab Portunus trituberculatus (Miers, 1876) (Decapoda, Portunidae)

In: Crustaceana
View More View Less
  • 1 Laboratory of Fish and Shellfish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, P.R. China
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

€29.95$34.95

Abstract

The sterol regulatory element-binding proteins (SREBPs) are a family of transcription factors known to activate the transcription of genes encoding key lipogenic enzymes. The present study reports on the molecular cloning, tissue expression and nutritional regulation of SREBP-1 from the swimming crab Portunus trituberculatus (Miers, 1876). The SREBP-1 full-length cDNA was 3785 bp, encoding a polypeptide of 1039 amino acids. Quantitative PCR analysis revealed that SREBP-1 expression was detected in various tissues, and the significantly higher expression levels were found in eyestalk and hepatopancreas compared with other tested tissues. Additionally, the effects of dietary iron on expression of SREBP-1 were investigated, and the results indicated that SREBP-1 expressions were down-regulated by crabs fed diets containing 218.9 and 373.9 mg/kg iron compared with that fed the basal diet (55.2 mg/kg). Suggesting that the relative expression level of SREBP-1 can be suppressed by dietary iron supplementation. These findings provide further insight into the regulatory capacity of SREBP-1 in the lipid anabolism of P. trituberculatus.

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 155 154 8
Full Text Views 4 4 0
PDF Views & Downloads 8 8 0