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Transcriptome analysis of Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) in response to CO2-driven acidification

In: Crustaceana
Authors:
Qi Shen Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, P.R. China

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Wei Xin Wang Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, P.R. China
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, P.R. China

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Hai Gang Chen Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, P.R. China

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Hong Geng Zhu Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, P.R. China

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Jian Hua Chen Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, P.R. China
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, P.R. China
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, P.R. China

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Huan Gao Jiangsu Key Laboratory of Marine Biotechnology/College of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, P.R. China
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, P.R. China

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Abstract

The effects of ocean acidification on marine organisms are of increasing concern. Exopalaemon carinicauda is an important economic shrimp. However, little is known about the transcriptome data for shrimp in response to seawater acidification stress. In this study, the transcriptome of E. carinicauda in response to seawater acidification stress was recorded using the Illumina RNA-sequencing. A total of 59 990 unigenes from high-quality transcripts were generated. Of all annotated unigenes, 18 386 and 17 681 unigenes had significant matches with sequences in the NR, and GO databases, respectively. A total of 183 differentially expressed genes (DEGs) could be screened, of which 119 DEGs were up-regulated and 64 DEGs were down-regulated. KEGG enrichment analysis showed these DEGs were primarily enriched in the pathways of lysosome, carbohydrate digestion and absorption, apoptosis, and alpha-linolenic acid metabolism. These results indicate that seawater acidification stress leads to the activation of apoptosis and the activity of the energy metabolism system in order to resist the external environmental stress and ensure the continuity of the normal life metabolism, and thus the energy supply of the organism. These data will be helpful to further study the molecular mechanisms of shrimp resistance to seawater acidification stress.

Résumé

Les effets de l’acidification des océans sur les organismes marins sont une préoccupation croissante. Exopalaemon carinicauda est une crevette économiquement importante. Cependant, peu de choses sont connues sur son transcriptome en réponse à un stress d’acidification de l’eau de mer. Dans cette étude, le transcriptome de E. carinicauda en réponse à un stress d’acidification marine a été étudié par séquençage d’ARN Illumina. Un total de 59 990 uni-gènes de transcrits de haute qualité a été généré. Parmi tous les uni-gènes annotés, 18 386 et 17 681 montraient une correspondance significative avec, respectivement, des séquences des bases de données NR et GO. Un total de 183 gènes différentiellement exprimés (DEGs) peuvent être criblés, parmi lesquels 119 DEGs ont été régulés à la hausse et 64 à la baisse. Les analyses d’enrichissement KEGG ont montré que ces DEGs étaient principalement enrichis dans les voies des lysosomes, la digestion et l’absorption des glucides, l’apoptose et le métabolisme de l’acide alpha-linolénique. Ces résultats montrent que le stress d’acidification de l’eau de mer conduit à une activation de l’apoptose et de l’activité du métabolisme énergétique afin de résister au stress environnemental externe et assurer la continuité d’un métabolisme de vie normal, et donc de l’apport d’énergie à l’organisme. Ces données seront utiles aux futures études des mécanismes moléculaires de la résistance des crevettes à un stress d’acidification de l’eau de mer.

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