Haemocyte characterization in invasive Carcinus maenas (Linnaeus, 1758) (Decapoda, Portunidae) from unpolluted vs polluted sites in Argentina
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Abstract
Pollution, biological invasions and climate change pose the greatest threats to biodiversity worldwide. Few studies explore the interlink between invasive species and local pollution sites. This study describes the type and cytochemical properties of haemocytes from invasive Carcinus maenas on the southwestern Atlantic coast (Argentina). Moreover, the morphology of haemocytes and the total haemocyte count (THC) as well as the differential haemocyte count (DHC) of crabs sampled at clean or unpolluted and polluted sites were compared, to determine whether environmental stresses were evident in their haemocytes. Our results revealed two groups or subpopulations of haemocytes: hyalinocytes, as well as granulocytes and semi-granulocytes. The type of haemocyte from crabs from both sites showed no differences in their morphology. For cytochemical assays, only neutral red dye showed a more intense staining of crab haemocytes at the polluted site. All haemocytes were positive to Periodic Acid-Schiff and Sudan Black. THC of crabs from the clean site were significantly higher, as well as having a significantly higher proportion of hyalinocytes and a lower proportion of granular cells. This study provides a baseline immunological assessment for C. maenas haemocytes, suggesting a differential haemocytic response to environmental stress.
Resumen
La contaminación, las invasiones biológicas y el cambio climático representan las mayores amenazas para la biodiversidad en todo el mundo. Pocos estudios exploran la interrelación entre las especies invasoras y los sitios de contaminación local. Este estudio describe el tipo y las propiedades citoquímicas de los hemocitos del invasor Carcinus maenas en la costa suroeste del Atlántico (Argentina). Además, se comparó la morfología de los hemocitos y el recuento total de hemocitos (THC) y el recuento diferencial de hemocitos (DHC) de cangrejos muestreados en sitios limpios o no contaminados y contaminados, para determinar si el estrés ambiental era evidente en sus hemocitos. Nuestros resultados revelaron dos grupos o subpoblaciones de hemocitos: hialinocitos y granulocitos y semigranulocitos. El tipo de hemocito de los cangrejos de ambos sitios no mostró diferencias en su morfología. Para los ensayos citoquímicos, solo el tinte rojo neutro mostró una tinción más intensa de los hemocitos de cangrejo en el sitio contaminado. Todos los hemocitos fueron positivos a PeriodicAcid-Schiff y Sudan Black. El THC de los cangrejos del sitio limpio fue significativamente mayor, además de tener una proporción significativamente mayor de hialinocitos y una menor proporción de células granulares. Este estudio proporciontabña una evaluación inmunológica de referencia para los hemocitos de C. maenas, lo que sugiere una respuesta hemocítica diferencial al estrés ambiental.
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Haemocyte characterization in invasive Carcinus maenas (Linnaeus, 1758) (Decapoda, Portunidae) from unpolluted vs polluted sites in Argentina
In: CrustaceanaSearch for other papers by Antonella C. Frizzera in
Current site
Google Scholar
PubMed
Search for other papers by Clara G. Samartino in
Current site
Google Scholar
PubMed
Search for other papers by Aldo M. Santo in
Current site
Google Scholar
PubMed
Search for other papers by Jamie Bojko in
Current site
Google Scholar
PubMed
Search for other papers by Florencia Cremonte in
Current site
Google Scholar
PubMed
Search for other papers by Nuria Vázquez in
Current site
Google Scholar
PubMed
Abstract
Pollution, biological invasions and climate change pose the greatest threats to biodiversity worldwide. Few studies explore the interlink between invasive species and local pollution sites. This study describes the type and cytochemical properties of haemocytes from invasive Carcinus maenas on the southwestern Atlantic coast (Argentina). Moreover, the morphology of haemocytes and the total haemocyte count (THC) as well as the differential haemocyte count (DHC) of crabs sampled at clean or unpolluted and polluted sites were compared, to determine whether environmental stresses were evident in their haemocytes. Our results revealed two groups or subpopulations of haemocytes: hyalinocytes, as well as granulocytes and semi-granulocytes. The type of haemocyte from crabs from both sites showed no differences in their morphology. For cytochemical assays, only neutral red dye showed a more intense staining of crab haemocytes at the polluted site. All haemocytes were positive to Periodic Acid-Schiff and Sudan Black. THC of crabs from the clean site were significantly higher, as well as having a significantly higher proportion of hyalinocytes and a lower proportion of granular cells. This study provides a baseline immunological assessment for C. maenas haemocytes, suggesting a differential haemocytic response to environmental stress.
Resumen
La contaminación, las invasiones biológicas y el cambio climático representan las mayores amenazas para la biodiversidad en todo el mundo. Pocos estudios exploran la interrelación entre las especies invasoras y los sitios de contaminación local. Este estudio describe el tipo y las propiedades citoquímicas de los hemocitos del invasor Carcinus maenas en la costa suroeste del Atlántico (Argentina). Además, se comparó la morfología de los hemocitos y el recuento total de hemocitos (THC) y el recuento diferencial de hemocitos (DHC) de cangrejos muestreados en sitios limpios o no contaminados y contaminados, para determinar si el estrés ambiental era evidente en sus hemocitos. Nuestros resultados revelaron dos grupos o subpoblaciones de hemocitos: hialinocitos y granulocitos y semigranulocitos. El tipo de hemocito de los cangrejos de ambos sitios no mostró diferencias en su morfología. Para los ensayos citoquímicos, solo el tinte rojo neutro mostró una tinción más intensa de los hemocitos de cangrejo en el sitio contaminado. Todos los hemocitos fueron positivos a PeriodicAcid-Schiff y Sudan Black. El THC de los cangrejos del sitio limpio fue significativamente mayor, además de tener una proporción significativamente mayor de hialinocitos y una menor proporción de células granulares. Este estudio proporciontabña una evaluación inmunológica de referencia para los hemocitos de C. maenas, lo que sugiere una respuesta hemocítica diferencial al estrés ambiental.
| All Time | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 216 | 87 | 3 |
| Full Text Views | 11 | 6 | 0 |
| PDF Views & Downloads | 21 | 9 | 1 |