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Identification and functional characterization of WNT5 in the ovarian maturation of Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae)

In: Crustaceana
Authors:
Xiao F. Lai School of Marine Science and Fisheries, Jiangsu Key Laboratory of Marine Bioresources and Environment / Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Marine Resource Development institute of Jiangsu (Lianyungang), Lianyungang 222005, P.R. China
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, P.R. China

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https://orcid.org/0000-0002-6038-1824
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Ting T. Shi School of Marine Science and Fisheries, Jiangsu Key Laboratory of Marine Bioresources and Environment / Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China

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Hao Chen School of Marine Science and Fisheries, Jiangsu Key Laboratory of Marine Bioresources and Environment / Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China

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Shan R. Shen School of Marine Science and Fisheries, Jiangsu Key Laboratory of Marine Bioresources and Environment / Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Marine Resource Development institute of Jiangsu (Lianyungang), Lianyungang 222005, P.R. China
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, P.R. China

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Huan Gao School of Marine Science and Fisheries, Jiangsu Key Laboratory of Marine Bioresources and Environment / Key Laboratory of Marine Biological Resources and Environment of Jiangsu Province, Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, P.R. China
Marine Resource Development institute of Jiangsu (Lianyungang), Lianyungang 222005, P.R. China
The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, P.R. China

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Ge T. Wu Jiangsu Youshi Biotechnology Development Co., Ltd, Suqian, Jiangsu 223831, P.R. China

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Abstract

The Wnt family, the ligand of the Wnt signal pathway, is highly conserved and plays an important role in various cellular processes including reproduction and development. In this study, wnt5 was cloned from the ridgetail white prawn Exopalaemon carinicauda (Ec-wnt5) and its function in ovarian maturation was investigated. The results showed that the Ec-wnt5 cDNA was 2422 bp, including an ORF with 1116 bp encoding a polypeptide with 371 amino acids. Ec-WNT5 had the highest homology with WNT5 from Penaeus vannamei (93.1%). Ec-wnt5 was expressed in 9 tissues with the most expression in ovary and increased significantly ( p < 0.05) along with ovarian maturation, while decreased significantly ( p < 0.05) with vitellogenin and cathepsin L in E. carinicauda ovary post Ec-wnt5 RNA interference. All gonadosmatic indices of the RNAi group were significantly lower than those of the control ( p < 0.05). The results suggest that Ec-WNT5 might be the key member of the Wnt family involved in ovarian maturation.

Résumé

La famille de Wnt, le ligand de la voie de signalisation Wnt, est fortement conservée et joue un rôle important dans divers processus cellulaires incluant la reproduction et le développement. Dans cette étude, wnt5 a été cloné à partir de la crevette bouquet Exopalaemon carinicauda (Ec-wnt5) et ses fonctions dans la maturation ovarienne ont été étudiées. Les résultats montrent que l’ADNc de Ec-wnt5 est de 2422 pb, incluant un ORF (cadre de lecture ouvert) de 1116 pb codant un polypeptide de 371 acides amines. Ec-WNT5 montre la plus forte homologie avec WNT5 de Penaeus vannamei (93,1%). Ec-wnt5 a été exprimé dans 9 tissus avec la plus forte expression dans l’ovaire et a augmenté significativement ( p < 0 , 05) au cours de la maturation ovarienne, tandis qu’il a décru significativement ( p < 0 , 05) avec vitellogenin et cathepsin L dans l’ovaire de E. carinicauda après interférence par ARN post Ec-wnt5. Tous les indices gonadosomatiques du groupe ARNi ont été plus faibles que ceux des témoins ( p < 0 , 05). Les résultats suggèrent que Ec-WNT5 pourrait être le membre clé de la famille Wnt impliqué dans la maturation ovarienne.

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