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Expression of the senescence-related gene FoxO in Daphnia pulex and its role in the regulation of reproductive transformation (Branchiopoda, Cladocera)

In: Crustaceana
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Shun Zhang School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China

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https://orcid.org/0000-0002-2769-1257
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Xuan-Yu Zhou School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China

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Cheng Zhang School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China

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Chun-Pu Zhao School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China

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Wei Li School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China

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Dan-Li Wang School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China

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Shan-Liang Xu School of Marine Science, Ningbo University, Zhejiang, Ningbo 315211, P.R. China
Key Laboratory of Applied Marine Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, P.R. China

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Abstract

The gene FoxO plays an important role in regulating individual growth and development, cell survival, and senescence, mainly through transcriptional regulation and the signal transduction pathway. In this study, the full-length cDNA sequence of the FoxO gene was cloned from Daphnia pulex. FoxO was silenced by RNA interference. The expression levels of the senescence-related genes FoxO, Akt, and Sir2 were analysed before and after interference by real-time polymerase chain reaction and Western blotting. Meanwhile, the production of an ephippium in D. pulex was evaluated before and after interference. The expression of FoxO and protein abundance decreased after interference. The number of ephippia produced in the Akt interference group was higher than in the two control groups, and the expressions of the senescence-related genes Akt and Sir2 decreased after interference. The results indicate that FoxO regulated senescence, primarily through the phosphorylation of Akt and acetylation of Sir2, thereby affecting the reproductive transformation of D. pulex.

Résumé

Le gène FoxO joue un rôle important dans la régulation de la croissance individuelle, le développement, la survie cellulaire et la sénescence, essentiellement grâce à la régulation transcriptionnelle et la voie de transcription du signal. Dans cette étude, la séquence complète de l’ADNc du gène FoxO a été clonée chez Daphnia pulex. FoxO a été réduit au silence par ADN interférence. Les niveaux d’expression des gènes reliés à la sénescence FoxO, Akt, et Sir2 ont été analysés avant et après interférence par PCR et Western Blot. En même temps, la production d’éphippie par D. pulex a été évaluée avant et après l’interférence. Le nombre d’éphippies produites dans le groupe d’interférence Akt a été plus élevé que dans les deux groupes témoins, et les expressions des gènes reliés à la sénescence Akt et Sir2 ont diminué après interférence. Les résultats montrent que FoxO a régulé la sénescence principalement par la phosphorylation de Akt et l’acétylation de Sir2, affectant ainsi le changement de la reproduction de D. pulex.

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