A Video Technique for Imaging the Three-Dimensional Architecture of Wood

in IAWA Journal
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This paper describes a video micrographic technique to image the internal three-dimensional structure of wood. The technique uses high resolution video and an optical disk recorder to give immediate access to noise-free serially-recorded images in both still-frame and motion modes. By using a hydrophilie embedding medium, small woody tissue samples can be successfully embedded, sectioned at thicknesses that are useful for video micrography of the exposed tissue surface, and serially recorded to analyse three-dimensional architecture. The technique can be used by researchers working in wood anatomy, xylem development, and water transport. It also promises to be useful for studying the three-dimensional architecture of small, non-woody structures. Modifications of the technique make it useful for larger woody and non-woody material.

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