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Simple differential staining method of paraffin-embedded plant sections with safranin-alcian blue

In: IAWA Journal
Authors:
Shunamit Wolberg Eastern R&D Center, Milken Campus, Ariel, Israel

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Mor Haim Eastern R&D Center, Milken Campus, Ariel, Israel

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Ilana Shtein Eastern R&D Center, Milken Campus, Ariel, Israel
Department of Molecular Biology, Ariel University, Ariel 40700, Israel

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https://orcid.org/0000-0002-2281-3550
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Summary

We present here a modified protocol for safranin-alcian blue staining of paraffin-embedded plant samples. After deparaffination, the double staining uses a 2:1 mix of 1% safranin and 1% alcian blue. This method is less expensive than previously published double staining protocols as it does not require absolute ethanol. Moreover, safranin-alcian blue staining creates a range of hues that allows good cell type differentiation, it gives reproducible results for a wide variety of plant tissues and is easy to use. We hope it will be found useful both for research and teaching purposes.

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