Save

Practical guidelines for quantitative wood anatomy on Ginkgo biloba L.

In: IAWA Journal
Authors:
Weiwei Huang Collaborative Innovation Center of Sustainable Forestry in Southern China of Jiangsu Province, Nanjing Forestry University, N anjing 210037, P.R. China
Bamboo Research Institute, Nanjing Forestry University, Nanjing 210037, P.R. China
Department of Geosciences and Natural Resource Management, The University of Copenhagen, Rolighedsvej 23, DK-1958 Frederiksberg C, Denmark

Search for other papers by Weiwei Huang in
Current site
Google Scholar
PubMed
Close
https://orcid.org/0000-0002-5682-1989
and
Yueyi Li Bamboo Research Institute, Nanjing Forestry University, Nanjing 210037, P.R. China

Search for other papers by Yueyi Li in
Current site
Google Scholar
PubMed
Close
https://orcid.org/0000-0002-4206-7858
Download Citation Get Permissions

Access options

Get access to the full article by using one of the access options below.

Institutional Login

Log in with Open Athens, Shibboleth, or your institutional credentials

Login via Institution

Purchase

Buy instant access (PDF download and unlimited online access):

$40.00

Summary

Ginkgo biloba L., a long-lived relict species, has high ecological, cultural and economic value. Studying the quantitative wood anatomy of G. biloba is important to understand the relationships between their tree-growth and climate change, which could help in the protection of old G. biloba trees. Preparing high-quality wood thin sections is the premise of quantitative wood anatomy analysis. In addition, manual correction of image analysis is a time-consuming process. We therefore present a protocol on how to make G. biloba high-quality xylem thin sections, from sample preparation, sample embedding, blade selection to thickness selection. We also present a method to determine the necessary sample width and number of cores to establish reliable wood anatomical time series that both can capture climate signals and reduce the time used for image analysis. For microsection preparation, it is optimal to cut 20–30-μm-thick sections using Leica DB80 blades after applying the cornstarch solution on top of the sample surface. For image analysis, the reliable wood anatomical time series can be established when measuring six cores (trees) or above, and the environmental signals are captured when analyzing a 1.2-mm-wide radial strip along the increment core (deviation <2%) for the parameters cell density, mean lumen area, potential hydraulic conductivity and percentage of the conductive area. This paper provides practical guidance to successfully use quantitative wood anatomy methods on G. biloba. We hope our study will be helpful for future research on the quantitative wood anatomy of G. biloba and similar tree species.

Content Metrics

All Time Past Year Past 30 Days
Abstract Views 404 222 14
Full Text Views 252 83 2
PDF Views & Downloads 457 162 5