Double-Staining to Improve Visualisation of Wood Decay Hyphae in Wood Sections

in IAWA Journal
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Combining fluorescent-labeled wheat germ agglutinin (a chitin-specific lectin) with conventional histological stains offers a simple, efficient method for studying fungal hyphae in deteriorating wood. Cell walls stain dark red with safranin 0, providing excellent contrast for the green-fluorescing hyphae. Staining sections with brilliant vital red markedly enhances the visibility of fungal bore holes.

Double-Staining to Improve Visualisation of Wood Decay Hyphae in Wood Sections

in IAWA Journal

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