Activity of free fatty acids-rich non-polar fractions from fruits of Capsicum chinense var. Criolla and Jaguar

In: Israel Journal of Plant Sciences

Plant-essential oils have been considered as an important source of bioactive molecules like antimicrobials, analgesics, anti-inflammatory and anti-carcinogen agents. Biological functions of plant extracts from the genus Capsicum are unknown. In the present work, non-polar fractions of ripe and unripe fruits of Capsicum chinense Jacq. Cultivar (cv.) Jaguar and Criollo were obtained by hexane-batch extraction and tested for antimicrobial activity against Gram-negative bacterial strain Escherichia coli (ATCC 25922), Gram-positive bacterial strains Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 12228), and yeast Candida albicans (ATCC 90028). Non-polar fractions from ripe fruits for both cv. exhibited greater antimicrobial activity compared to unripe fruits. Implication of numbered FFA’s on observed antimicrobial activity are discussed.

  • Addison R.F. Ackman R.G. (1970). Exceptional occurrence of odd-chain fatty acids in smelt (Osmerus mordax) from Jeddore Harbour, Nova Scotia. Lipids5(6): 554557. doi:10.1007/bf02532744 .

    • Search Google Scholar
    • Export Citation
  • Akthar M.S. Degaga B. Azam T. (2014). Antimicrobial activity of essential oils extracted from medicinal plants against the pathogenic microorganisms: a review. Issues in Biological Sciences and Pharmaceutical Research2(1): 17 .

    • Search Google Scholar
    • Export Citation
  • Alsabri S.G. El-Basir H.M. Rmeli N.B. Mohamed S.B. Allafi A.A. Zetrini A.A. Salem A.A. Mohamed S.S. Gbaj A. El-Baseir M.M. (2013). Phytochemical screening, antioxidant, antimicrobial and anti-proliferative activities study of Arbutus pavarii plant. Journal of Chemical and Pharmaceutical Research5(1): 3236 .

    • Search Google Scholar
    • Export Citation
  • Anisimov M.M. Gerasimenko N.I. Chaǐkina E.L. Serebriakov I.M. (2009). Biological activity of metabolites of the herb Kalanchoe diagremontania (Hamet de la Bbathie) Jacobs et Perr. Izvestiia Akademii nauk. Seriia Biologicheskaia6: 669676 .

    • Search Google Scholar
    • Export Citation
  • Babic I. Nguyen-the C. Amiot M.J. Aubert S. (1994). Antimicrobial activity of shredded carrot extracts on food-borne bacteria and yeast. Journal of Applied Bacteriology76(2): 135141. doi:10.1111/j.1365-2672.1994.tb01608.x .

    • Search Google Scholar
    • Export Citation
  • Ben Jannet H. Chaari A. Bakhrouf A. Mighri Z. (2006). Structure-antibacterial activity relationship of secondary metabolites from Ajuga pseudoiva Rob. leaves. Natural Product Research20(3): 299304. doi:10.1080/14786410500129129 .

    • Search Google Scholar
    • Export Citation
  • Bhagat R.B. Kulkarni D.K. (2010). In vitro antimicrobial activity, lipid content and physico-chemical analysis of non-edible oils. Asian Journal of Chemistry22(9): 68846890 .

    • Search Google Scholar
    • Export Citation
  • Cetinkaya Y. Falk P. Mayhall C.G. (2000). Vancomycin-resistant enterococci. Clinical microbiology reviews13(4): 686707. doi:10.1128/CMR.13.4.686 .

    • Search Google Scholar
    • Export Citation
  • Cowan M.M. (1999). Plant products as antimicrobial agents. Clinical microbiology reviews12(4): 564582. doi:10.1128/CMR.12.4.564 .

  • Da Silva P.E.A. Palomino J.C. (2011). Molecular basis and mechanisms of drug resistance in Mycobacterium tuberculosis: classical and new drugs. Journal of antimicrobial chemotherapy66(7): 14171430. doi:10.1093/jac/dkr173 .

    • Search Google Scholar
    • Export Citation
  • Desbois A.P. Mearns-Spragg A. Smith V.J. (2009). A fatty acid from the diatom Phaeodactylum tricornutum is antibacterial against diverse bacteria including multi-resistant Staphylococcus aureus (MRSA). Marine Biotechnology11(1): 4552. doi:10.1007/s10126-008-9118-5 .

    • Search Google Scholar
    • Export Citation
  • Desbois A.P. Smith V.J. (2010). Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology85(6): 16291642. doi:10.1007/s00253-009-2355-3 .

    • Search Google Scholar
    • Export Citation
  • Enright M.C. Robinson D.A. Randle G. Feil E.J. Grundmann H. Spratt B.G. (2002). The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proceedings of the National Academy of Sciences99(11): 76877692. doi:10.1073/pnas.122108599 .

    • Search Google Scholar
    • Export Citation
  • Farmer E.E. Weber H. Vollenweider S. (1998). Fatty acid signaling in Arabidopsis. Planta206(2): 167174. doi:10.1007/s004250050388.

    • Search Google Scholar
    • Export Citation
  • Feng S. Saw C.L. Lee Y.K. Huang D. (2007). Fungal-stressed germination of black soybeans leads to generation of oxooctadecadienoic acids in addition to glyceollins. Journal of Agricultural and Food Chemistry55(21): 85898595. doi:10.1021/jf0716735 .

    • Search Google Scholar
    • Export Citation
  • Hilditch T.P. Williams P.N. (1964). The Chemical Constitution of Natural Fats4th ed. New York, USA: John Willey & Sons, Inc. pp. 745.

    • Search Google Scholar
    • Export Citation
  • Hood J.R. Wilkinson J.M. Cavanagh H.M. (2003). Evaluation of common antibacterial screening methods utilized in essential oil research. Journal of Essential Oil Research15(6): 428433. doi:10.1080/10412905.2003.9698631 .

    • Search Google Scholar
    • Export Citation
  • Huie C. (2002). A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Analytical and Bioanalytical Chemistry373(1-2): 2330. doi:10.1007/s00216-002-1265-3 .

    • Search Google Scholar
    • Export Citation
  • Isbell T.A. Carlson K.D. Abbott T.P. Phillips B.S. Erhan S.M. Kleiman R. (1996). Isolation and characterization of wax esters in meadowfoam oil. Industrial Crops and Products5(3): 239243. doi:10.1016/0926-6690(96)89456-8 .

    • Search Google Scholar
    • Export Citation
  • Kachroo A. Kachroo P. (2009). Fatty acid-derived signals in plant defense. Annual Review of Phytopathology47: 153176. doi:10.1146/annurev-phyto-080508-081820 .

    • Search Google Scholar
    • Export Citation
  • Kattner G. Hagen W. Graeve M. Albers C. (1998). Exceptional lipids and fatty acids in the pteropod Clione limacina (Gastropoda) from both polar oceans. Marine Chemistry61(3–4): 219228. doi:10.1016/S0304-4203(98)00013-9 .

    • Search Google Scholar
    • Export Citation
  • Koch R. (1881). Über Desinfektions. Mittheil. des Kaiserl. Gesundheitsamtes1: 234282 .

  • Lieberman S. Enig M.G. Preuss H.G. (2006). A review of monolaurin and lauric acid: natural virucidal and bactericidal agents. Alternative and Complementary Therapies12(6): 310314. doi:10.1089/act.2006.12.310 .

    • Search Google Scholar
    • Export Citation
  • Manohar V. Echard B. Perricone N. Ingram C. Enig M. Bagchi D. Preuss H.G. (2013). In vitro and in vivo effects of two coconut oils in comparison to monolaurin on Staphylococcus aureus: rodent studies. Journal of Medicinal Food16(6): 499503. doi:10.1089/jmf.2012.0066 .

    • Search Google Scholar
    • Export Citation
  • McGaw L. Jäger A. Van Staden J. Houghton P. (2002). Antibacterial effects of fatty acids and related compounds from plants. South African journal of botany68(4): 417423. doi:10.1016/S0254-6299(15)30367-7 .

    • Search Google Scholar
    • Export Citation
  • Morrison W.R. and Smith L.M. (1964). Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol. Journal of lipid research5(4): 600608 .

    • Search Google Scholar
    • Export Citation
  • Mueller M. Hobiger S. Jungbauer A. (2010). Anti-inflammatory activity of extracts from fruits, herbs and spices. Food Chemistry122(4): 987996. doi:10.1016/j.foodchem.2010.03.041

    • Search Google Scholar
    • Export Citation
  • Murata N. Sato N. Takahashi N. (1984). Very-long-chain saturated fatty acids in phosphatidylserine from higher plant tissues. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism795(1): 147150. doi:10.1016/0005-2760(84)90115-2 .

    • Search Google Scholar
    • Export Citation
  • Nazif N.M. (2002). Phytoconstituents of Zizyphus spina-christi L. fruits and their antimicrobial activity. Food Chemistry76(1): 7781. doi:10.1016/s0308-8146(01)00243-6 .

    • Search Google Scholar
    • Export Citation
  • Oliveira I. Sousa A. Morais J.S. Ferreira I.C.F.R. Bento A. Estevinho L. Pereira J.A. (2008). Chemical composition, and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food and Chemical Toxicology46(5): 18011807. doi:10.1016/j.fct.2008.01.026.

    • Search Google Scholar
    • Export Citation
  • Pan S.-Y. Zhou S.-F. Gao S.-H. Yu Z.-L. Zhang S.-F. Tang M.-K. Sun J.-N. Ma D.-L. Han Y.-F. Fong W.-F. Ko K.-M. (2013). New perspectives on how to discover drugs from herbal medicines: CAM’s outstanding contribution to modern therapeutics. Evidence-Based Complementary and Alternative Medicine2013: 25. doi:10.1155/2013/627375.

    • Search Google Scholar
    • Export Citation
  • Pérez-Gálvez A. Garrido-Fernández J. Mínguez-Mosquera M.I. Lozano-Ruiz M. Montero-de-Espinosa V. (1999). Fatty acid composition of two new pepper varieties (Capsicum annuum L. cv. Jaranda and Jariza). Effect of drying process and nutritional aspects. Journal of the American Oil Chemists’ Society76(2): 205208. doi:10.1007/s11746-999-0219-8 .

    • Search Google Scholar
    • Export Citation
  • Sands J.A. Auperin D.D. Landin P.D. Reinhardt A. Cadden S.P. (1978). Antiviral effects of fatty acids & derivatives: lipid-containing bacteriophages as a model system. In: Kabara J.J. (ed.) The Pharmacological Effects of Lipids Vol. 1. Illinois, USA: American Oils Chemists’ Society pp. 7595.

    • Search Google Scholar
    • Export Citation
  • Seidel V. Taylor P.W. (2004). In vitro activity of extracts and constituents of Pelagonium against rapidly growing mycobacteria. International Journal of Antimicrobial Agents23(6): 613619. doi:10.1016/j.ijantimicag.2003.11.008 .

    • Search Google Scholar
    • Export Citation
  • Tamokou J.D.D. Simo Mpetga D.J. Keilah Lunga P. Tene M. Tane P. Kuiate J.R. (2012). Antioxidant and antimicrobial activities of ethyl acetate extract, fractions and compounds from stem bark of Albizia adianthifolia (Mimosoideae). BMC Complementary and Alternative Medicine12. doi:10.1186/1472-6882-12-99 .

    • Search Google Scholar
    • Export Citation
  • Tolan I. Ragoobirsingh D. Morrison E.Y. (2004). Isolation and purification of the hypoglycaemic principle present in Capsicum frutescens . Phytotherapy Research18: 9596. doi:10.1002/ptr.1328 .

    • Search Google Scholar
    • Export Citation
  • Tsuchiya H. (2001). Biphasic membrane effects of capsaicin, an active component in Capsicum species. Journal of Ethnopharmacology75(2–3): 295299. doi:10.1016/S0378-8741(01)00200-8 .

    • Search Google Scholar
    • Export Citation
  • Tsydendambaev V.D. Christie W.W. Brechany E.Y. Vereshchagin A.G. (2004). Identification of unusual fatty acids of four alpine plant species from the Pamirs. Phytochemistry65(19): 26952703. doi:10.1016/j.phytochem.2004.08.021.

    • Search Google Scholar
    • Export Citation
  • Wille J. Kydonieus A. (2003). Palmitoleic acid isomer (C16: 1Δ6) in human skin sebum is effective against gram-positive bacteria. Skin Pharmacology and Physiology16(3): 176187. doi:10.1159/000069757.

    • Search Google Scholar
    • Export Citation
  • Wu H. Guo J. Chen S. Liu X. Zhou Y. Zhang X. Xu X. (2013). Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis72(0): 267291. doi:10.1016/j.jpba.2012.09.004 .

    • Search Google Scholar
    • Export Citation
  • Yff B.T. Lindsey K.L. Taylor M.B. Erasmus D.G. Jäger A.K. (2002). The pharmacological screening of Pentanisia prunelloides and the isolation of the antibacterial compound palmitic acid. Journal of Ethnopharmacology79(1): 101107. doi:10.1016/S0378-8741(01)00380-4.

    • Search Google Scholar
    • Export Citation
  • Zhukov A.V. and Vereshchagin A.G. (1970). Heptadecenoic acid as an internal standard in the gas chromatographic weight determination of fatty acids. Journal of Chromatography A51(0): 155166. doi:10.1016/S0021-9673(01)96850-5.

    • Search Google Scholar
    • Export Citation

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