Epicypta is treated in the sense employed by Edwards (1925) under the name Delopsis Skuse. The species of Epicypta in the Holarctic region are discussed and keyed. Type material of Mycetophila aterrima Zett., M. selecta Walker, M. vitrea Coquillett and Epicypta pulicaria Loew has been examined and found to be conspecific. Lectotypes are designated for aterrima, vitrea and pulicaria. Mycetophila anomala Johannsen (holotype examined) is a distinct species but a new name, limnophila nom.n. is proposed as it is a homonym. Both aterrima and limnophila are Holarctic. Four other European species, scatophora (Perris), fumigata (Dziedzicki), torquata Matile and nigrobasis (Dziedzicki) (last not examined) are recognised. These six species and the Japanese ornatipennis (Okada) (not examined) belong to one species group related to Oriental and Afrotropical species. Two new species, lepida n.sp. and helvopicta n.sp., described from north America, belong to different, otherwise Neotropical, species groups. Descriptions and figures are given for all species known from the Holarctic, except nigrobasis and ornatipennis.