Thirteen species of Chloropidae are recorded from the Galápagos Islands, Ecuador. Seven new species are described: Diplotoxa loma sp. n. (subfamily Chloropinae); Conioscinella empheria sp. n.; Gaurax gethosyne sp. n.; Hippelates alyscus sp. n.; Liohippelates baptipalpis sp. n.; Olcella anaclasta sp. n.; Olcella lupina sp. n. (subfamily Oscinellinae). Another species in the genus Apallates is apparently undescribed, but there is insufficient information to justify a formal description. Monochaetoscinella anonyma is recorded for the first time from the archipelago. Four species previously recorded from the archipelago were also identified: Cadrema pallida; Conioscinella galapagensis; Elachiptera cultrata; Liohippelates galapagensis. Previously published Galápagos records of Liohippelates pusio apparently refer to L. galapagensis. A key to the Galápagos species of Chloropidae is given. Geographic affinities of the Galápagos chloropid fauna are similar to those of other Diptera from the archipelago, with few pantropical species, some species also found in the northern Neotropical and southern Nearctic regions, and endemic species apparently with Neotropical sister groups.