Ancient DNA Analysis of the Thulamela Remains: Deciphering the Migratory Patterns of a Southern African Population

In: Journal of African Archaeology

Abstract

Ancient DNA (aDNA) analysis was employed to obtain information on the population relationships of the two Thulamela individuals (AD 1400-1700) and six other skeletons from various archaeological sites of the southern African Iron Age – Tuli (Botswana), Nwanetsi, Makgope, Happy Rest and Stayt. Although sequences were short, it seems that the Thulamela female aligns somewhat more with eastern populations as opposed to the male who aligns more with western groups. This result is not surprising given that the two individuals were buried at the same site but their burials were hundreds of years apart. It was also possible to identify genetic links between the Iron Age individuals and modern southern African populations (e.g. some of the skeletons assessed showed maternal genetic similarities to present-day Sotho/Tswana groups) and to separate the samples into at least two genetic groups. Poor quality and quantity of DNA meant that only haplogroups, not subhaplogroups, of the individuals could be traced.

  • Adachi, N., Shinoda, K., Umetsu, K., & Matsumura, H. 2009. Mitochondrial DNA Analysis of Jomon skeletons from the Funadomari site, Hokkaido, and its Implication for the origins of Native Americans. American Journal of Physical Anthropology 138 (3), 255-265.

    • Search Google Scholar
    • Export Citation
  • Badenhorst, S. 2010. Descent of Iron Age farmers in southern Africa during the last 2000 Years. African Archaeological Review 27 (2), 87-106.

    • Search Google Scholar
    • Export Citation
  • Berniell-Lee, G., Calafell, F., Bosch, E., Heyer, E., Sica, L., & Mouguiama-Daouda, P. 2009. Genetic and demographic implications of the Bantu expansion: insights from human paternal lineages. Molecular Biology and Evolution 26 (7), 1581-1589.

    • Search Google Scholar
    • Export Citation
  • Castri, L., Tofanelli, S., Garagnani, P., Bini, C., Fosella, X., & Pelotti, S. 2009. mtDNA variability in two Bantu-Speaking populations (Shona and Hutu) from Eastern Africa: Implications for peopling and migration patterns in Sub-Saharan Africa. American Journal of Physical Anthropology 140 (2), 302-311.

    • Search Google Scholar
    • Export Citation
  • Denbow, J. 1986. A new look at the later prehistory of the Kalahari. Journal of African History 27 (1), 3-29.

  • Destro-Bisol, G., Donati, F., Coia, V., Boschi, I., Verginelli, F., & Caglia, A. 2004. Variation of female and male lineages in sub-Saharan populations: the importance of sociocultural factors. Molecular Biology and Evolution 21 (9), 1673-1682.

    • Search Google Scholar
    • Export Citation
  • Friedling, L.J., & Morris, A.G. 2005. The frequency of culturally derived dental modification practices on the Cape Flats in the Western Cape. South African Dental Journal 60 (3), 97-102.

    • Search Google Scholar
    • Export Citation
  • Friedling, L.J., & Morris, A.G. 2007. Pulling teeth for fashion: dental modification in modern day Cape Town, South Africa. South African Dental Journal 62 (3), 106-113.

    • Search Google Scholar
    • Export Citation
  • Gruen, R., Brink, J.S., Spooner, N.A., Taylor, L., Stringer, C.B., Franciscus, R.G., & Murray, A.S. 1996. Direct dating of Florisbad hominid. Nature 382 (6591), 500.

    • Search Google Scholar
    • Export Citation
  • Henn, B.M., Gignoux, C.R., Jobin, M., Granka, J.M., Macpherson, J.M., Kidd, J.M., Rodríguez-Botigué, L., Ramachandran, S., Hon, L., Brisbin, A., & Lin, A.A. 2011. Hunter gatherer genomic diversity suggests a southern African origin for modern humans. Proceedings of the National Academy of Sciences 108 (13), 5154-5162.

    • Search Google Scholar
    • Export Citation
  • Huffman, T.N. 2009. Mapungubwe and Great Zimbabwe: The origin and spread of social complexity in southern Africa. Journal of Anthropological Archaeology 28 (1), 37-54.

    • Search Google Scholar
    • Export Citation
  • Huffman, T.N. 1986. Archaeological evidence and conventional explanations of southern Bantu settlement patterns. Africa 56 (3), 280-298.

    • Search Google Scholar
    • Export Citation
  • Huffman, T.N. 1989. Ceramics, settlements and Late Iron Age migrations. African Archaeological Review 7 (1), 155-182.

  • Huffman, T.N. 2007. Handbook to the Iron Age: The Archaeology of Pre-Colonial Farming Societies in Southern Africa. University of KwaZulu-Natal Press, Scottsville.

    • Search Google Scholar
    • Export Citation
  • Hutten, M., Nienaber, W.C., & Steyn, M. (no date) Investigation of two 13th century Iron Age graves from Stayt, Limpopo Province. unpublished report, University of Pretoria.

  • Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16 (2), 111-120.

    • Search Google Scholar
    • Export Citation
  • Krüttli, A., Bouwman, A., Akgül, G., Della Casa, P., Rühli, F., & Warinner, C. 2014. Ancient DNA analysis reveals high frequency of European lactase persistence allele (T-13910) in Medieval central Europe. PLOS one 9 (1), e86251.

    • Search Google Scholar
    • Export Citation
  • Lane, A.B., Soodyall, H., Arndt, S., Ratshikhopha, M.E., Jonker, E., & Freeman, C. 2002. Genetic substructure in South African Bantu-speakers: evidence from autosomal DNA and Y-chromosome studies. American Journal of Physical Anthropology 119 (2), 175-185.

    • Search Google Scholar
    • Export Citation
  • Miller, S.M. 1998. The archaeological investigation, the rebuilding of the walls, and the public presentation of the Thulamela archaeological site. National Parks Board, Pretoria.

    • Search Google Scholar
    • Export Citation
  • Miller, S.M. 1990. Adaptation of Traditional Building Methods. Unpublished MA Thesis, University of the Witwatersrand.

  • Mooder, K.P., Weber, A.W., Bamforth, F.J., Lieverse, A.R., Schurr, T.G., & Bazaliiski, V.I. 2005. Matrilineal affinities and prehistoric Siberian mortuary practices: a case study from Neolithic Lake Baikal. Journal of Archaeological Science 32 (4) 619-634.

    • Search Google Scholar
    • Export Citation
  • Morris, A.G. 2005. Prehistory in blood and bone: an essay on the construction of the past from genetics and morphology. Transactions of the Royal Society of South Africa 60 (2) 111-114.

    • Search Google Scholar
    • Export Citation
  • Morris, A.G. 1992. A Master Catalogue: Holocene Human Skeletons from South Africa. Witwatersrand University Press, Johannesburg.

  • Morris, A.G. 1989. Dental mutilation in historic and prehistoric South Africa. Quarterly Bulletin of the South African Library 43 (3), 207-224.

    • Search Google Scholar
    • Export Citation
  • Morris, A.G., Heinze, A., Chan, E.K.F., Smith, A.B., & Hayes, V.M. 2014. First Ancient Mitochondrial Human Genome from a Pre-Pastoralist Southern African. Genome Biology and Evolution 6 (10), 2647-2653.

    • Search Google Scholar
    • Export Citation
  • Mosothwane, M.N. 2011. The Tuli Mummy: A preliminary report from Northeastern Botswana. Southern African Archaeological Bulletin 66 (194), 157-160.

    • Search Google Scholar
    • Export Citation
  • Newman, J.L. 1995. The Peopling of Africa: A Geographic Interpretation. Yale University Press, New Haven.

  • Nienaber, W.C., & Steyn, M. (no date) Nwanetsi. Kruger National Park. unpublished report, University of Pretoria.

  • Pääbo, S.H.P., Serre, D., Jaenicke-Després, V., Hebler, J., Rohland, N., & Kuch, M. 2004. Genetic analyses from ancient DNA. Annual Review of Genetics 38, 645-679.

    • Search Google Scholar
    • Export Citation
  • Phillipson, D.W. 1977. Spread of Bantu Language. Scientific American 236 (4), 106-114.

  • Pickrell, J.K., Patterson, N., Barbieri, C., Berthold, F., Gerlach, L., Güldemann, T., Kure, B., Mpoloka, S.W., Nakagawa, H., Naumann, C., & Lipson, M. 2012. The genetic prehistory of southern Africa. Nature Communications 3, 1143.

    • Search Google Scholar
    • Export Citation
  • Pistorius, J.C.C., Steyn, M., & Nienaber, W.C. 1998. Two burials from Makgope – a Batswana settlement in the Bankveld. South African Journal of Ethnology 21 (3), 115-124.

    • Search Google Scholar
    • Export Citation
  • Prinsloo, H.P., & Coetzee, F.P. 2001. Stayt: A 13th Century Iron Age Site, Soutpansberg District, Northern Province, South Africa. South African Journal of Ethnology, 24 (3), 81-87.

    • Search Google Scholar
    • Export Citation
  • Rühli, F.J., Steyn, M., Mosothwane, M.N., Öhrström, L., Bodiba, M.K., & Bouman, A. 2016. Radiological and genetic analysis of Late Iron Age mummy from the Tuli Block, Botswana. South African Journal of Science 112 (1-2), 1-7.

    • Search Google Scholar
    • Export Citation
  • Schlebusch, C.M., Lombard, M., & Soodyall, H. 2013. MtDNA control region variation affirms diversity and deep sub- structure in populations from Southern Africa. BMC Evolutionary Biology 13 (1), 56.

    • Search Google Scholar
    • Export Citation
  • Schlebusch, C.M., Malmström, H., Günther, T., Sjödin, P., Coutinho, A., Edlund, H., Munters, A.R., Steyn, M., Soodyall, H., Lombard, M., & Jakobsson, M. 2017. Ancient genomes from southern Africa pushes modern human divergence beyond 260,000 years ago. Science 358, 652-655.

    • Search Google Scholar
    • Export Citation
  • Skoglund, P., Thompson, J.C., Prendergast, M.E., Mittnik, A., Sirak, K., Hajdinjak, M., Salie, T., Rohland, N., Mallick, S., Peltzer, A., & Heinze, A. 2017. Reconstructing Prehistoric African Population Structure. Cell 171 (1) 59-71.

    • Search Google Scholar
    • Export Citation
  • Soodyall, H., & Jenkins, T. 1992a. Mitochondrial DNA studies in the South African Indian population. Gene Geography: A Computerised Bulletin on Human Gene Frequencies 6 (3) 127-137.

    • Search Google Scholar
    • Export Citation
  • Soodyall, H., & Jenkins, T. 1992b. Mitochondrial DNA polymorphisms in Khoisan populations from southern Africa. Annals of Human Genetics 56 (4), 315-324.

    • Search Google Scholar
    • Export Citation
  • Soodyall, H., Vigilant, L., Hill, A.V., Stoneking, M., & Jenkins, T. 1996. mtDNA control-region sequence variation suggests multiple independent origins of an “Asian-specific” 9-bp deletion in sub-Saharan Africans. American Journal of Human Genetics 58 (3), 595-608.

    • Search Google Scholar
    • Export Citation
  • Steyn, M., Loots, M., & Prinsloo, H. 1994. An Early Iron Age human skeleton from the Soutpansberg district, South Africa. South African Journal of Science 90 (10), 552-555.

    • Search Google Scholar
    • Export Citation
  • Steyn, M., Miller, S., Nienaber, W.C., & Loots, M. 1998. Late Iron Age gold burials from Thulamela (Pafuri Region, Kruger National Park). Southern African Archaeological Bulletin 53, 73-85.

    • Search Google Scholar
    • Export Citation
  • Van Reenen, J.F. 1978. Tooth mutilation amongst the peoples of Kavango and Bushmanland, South West Africa (Namibia). Journal of the Dental Association of South Africa 33 (4) 205-218.

    • Search Google Scholar
    • Export Citation

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