Fatty acid profile of black soldier fly larvae and frass as affected by different growing substrates
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Abstract
This study aimed to evaluate how different food wastes used as growth substrates affected the lipid composition of Hermetia illucens larvae; the relationship among substrates, larvae, and frass fatty acid (FA) composition was studied. Six thousand five-day-old Hermetia illucens larvae were allotted to one of four different substrates named CTRL (a control substrate made of broiler feed), V50 + B50 (vegetable and butchery wastes, 1:1 ratio), V75 + B25 (vegetable and butchery wastes, 3:1 ratio), and V100 (entirely composed of vegetables). Lipids were extracted from substrates, larvae, and frass separately and the FAs were quantified. Saturated FAs (SFAs) prevailed in all the groups. The CTRL larvae had the highest SFA (64.3%), while the V100 and V50 + B50 larvae had the lowest percentages. The V100 frass recorded a significantly higher SFA value (44.8%) than the others. Lauric acid (C12:0) was high in CTRL (0.2%) and V100 (0.3%) substrates and amount at 7.6 and 9.6% in their correspondent frass, respectively. However, C12:0 content was higher in the CTRL larvae than in the V100 ones (36.7 and 24.5%, respectively), while it had an intermediate value (28.9%) in the V75 + B25 larvae. Finally, the n-3 polyunsaturated FAs (PUFAs) level was high in the V100 substrate and larvae but not in the V100 frass. The relationship between frass FAs and their correspondent amounts in substrates and larvae was significant for C12:0, C18:2n-6, MUFAs, and n-6 PUFAs. There was a positive relationship for C12:0 and MUFAs with both substrates and larvae, while for C18:2n-6 and n-6 PUFAs, the relationship was positive for substrates but negative for larvae. In conclusion, the V100 substrate appeared to be the most suitable treatment for rearing Hermetia illucens because of the positive effects on the fatty acid content of larvae (low SFA and high n-3 PUFA content) and frass (optimal lauric acid levels for fertilizers).
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Addeo, N.F., Vozzo, S., Secci, G., Mastellone, V., Piccolo, G., Lombardi, P., Parisi, G., Asiri, K.A., Attia, Y.A. and Bovera, F., 2021. Different combinations of butchery and vegetable wastes on growth performance, chemical-nutritional characteristics and oxidative status of black soldier fly growing larvae. Animals 11: 3515. https://doi.org/10.3390/ani11123515
-
Akoh, C.C., 2017. Food lipids: chemistry, nutrition, and biotechnology. CRC Press, New York, NY, USA.
-
AOAC, 2007. Official methods of analysis. 18th ed. Association of Official Analytical Chemists, Arlington, VA, USA.
-
Attia, Y.A., Rahman, M.T., Hossain, M.J., Basiouni, S., Khafaga, A.F., Shehata, A.A. and Hafez, H.M., 2022. Poultry production and sustainability in developing countries under the COVID-19 crisis: lessons learned. Animals 12: 644. https://doi.org/10.3390/ani12050644
-
Borrelli, L., Varriale, L., Dipineto, L., Pace, A., Menna, L.F. and Fioretti, A., 2021. Insect derived lauric acid as promising alternative strategy to antibiotics in the antimicrobial resistance scenario. Frontiers in Microbiology 12. https://doi.org/10.3389/fmicb.2021.620798
-
Candian, V., Savio, C., Meneguz, M., Gasco, L. and Tedeschi, R., 2023. Effect of the rearing diet on gene expression of antimicrobial peptides in Hermetia illucens (Diptera: Stratiomyidae). Insect Science 30: 933-946. https://doi.org/10.1111/1744-7917.13165
-
Carroll, A., Fitzpatrick, M. and Hodge, S., 2023. The effects of two organic soil amendments, biochar and insect frass fertilizer, on shoot growth of cereal seedlings. Plants 12: 1071. https://doi.org/10.3390/plants12051071
-
Cattaneo, A., Meneguz, M. and Dabbou, S., 2023. The fatty acid composition of black soldier fly larvae: the influence of feed substrate and applications in the feed industry. Journal of Insects as Food and Feed. https://doi.org/10.1163/23524588-20230068
-
Chow, J.C., Watson, J.G., Lowenthal, D.H., Chen, L.W.A., Zielinska, B., Mazzoleni, L.R. and Magliano, K.L., 2007. Evaluation of organic markers for chemical mass balance source apportionment at the Fresno Supersite. Atmospheric Chemistry and Physics 7: 1741-1754. https://doi.org/10.5194/acp-7-1741-2007
-
Connell, J.J., 1975. Fishing organ weights of rainbow trout, Salmo gairdneri. Journal of Fish Biology 8: 489-499.
-
Dayrit, F.M., 2015. The properties of lauric acid and their significance in coconut oil. Journal of the American Oil Chemists’ Society 92: 1-15. https://doi.org/10.1007/s11746-014-2562-7
-
Dhama, K., Latheef, S.K., Dadar, M., Samad, H.A., Munjal, A., Khandia, R., Karthik, K., Tiwari, R., Yatoo, M.I., Bhatt, P., Chakraborty, S., Singh, K.P., Iqbal, H.M.N., Chaicumpa, W. and Joshi, S.K., 2019. Biomarkers in stress related diseases/disorders: diagnostic, prognostic, and therapeutic values. Frontiers in Molecular Biosciences 18: 91. https://doi.org/10.3389/fmolb.2019.00091
-
Dhanya, C.S., Paul, W., Rekha, M.R. and Joseph, R., 2023. Solid lipid nanoparticles of lauric acid: a prospective drug carrier for oral drug delivery. Journal of Molecular Liquids 380: 121738. https://doi.org/10.1016/j.molliq.2023.121738
-
Domı́nguez, R., Pateiro, M., Gagaoua, M., Barba, F.J., Zhang, W. and Lorenzo, J.M., 2019. A comprehensive review on lipid oxidation in meat and meat products. Antioxidants 8: 429. https://doi.org/10.3390/antiox8100429
-
Ewald, N., Vidakovic, A., Langeland, M., Kiessling, A., Sampels, S. and Lalander, C., 2020. Fatty acid composition of black soldier fly larvae (Hermetia illucens) – possibilities and limitations for modification through diet. Waste Management 102: 40-47. https://doi.org/10.1016/j.wasman.2019.10.014
-
FAO, 2021. Looking at edible insects from a food safety perspective. Challenges and opportunities for the sector. FAO, Rome, Italy. https://doi.org/10.4060/cb4094en
-
Folch, J., Lees, M. and Sloane Stanley, G.H., 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226: 497-509.
-
Franco, A., Scieuzo, C., Salvia, R., Petrone, A.M., Tafi, E., Moretta, A., Schmitt, E. and Falabella, P., 2021. Lipids from Hermetia illucens, an innovative and sustainable source. Sustainability 13: 10198. https://doi.org/10.3390/su131810198
-
Giannetto, A., Oliva, S., Lanes, C.F.C., de Araújo Pedron, F., Savastano, D., Baviera, C., Parrino, V., Lo Paro, G., Sapnò, N.C., Cappello, T., Maisano, M., Mauceri, A. and Fasulo, S., 2020. Hermetia illucens (Diptera: Stratiomydae) larvae and prepupae: biomass production, fatty acid profile and expression of key genes involved in lipid metabolism. Journal of Biotechnology 307: 44-54. https://doi.org/10.1016/j.jbiotec.2019.10.015
-
Hoc, B., Genva, M., Fauconnier, M.L., Lognay, G., Francis, F. and Caparros Megido, R., 2020. About lipid metabolism in Hermetia illucens (L. 1758): on the origin of fatty acids in prepupae. Scientific Reports 10: 11916. https://doi.org/10.1038/s41598-020-68784-8
-
Hurtado-Ribeira, R., Hernández, D.M., Villanueva-Bermejo, D., Garcı́a-Risco, M.R., Hernández, M.D., Vázquez, L., Fornari, T. and Martin, D., 2023. The interaction of slaughtering, drying, and defatting methods differently affects oxidative quality of the fat from black soldier fly (Hermetia illucens) larvae. Insects 14: 368. https://doi.org/10.3390/insects14040368
-
Jung, S., Lee, S., Lee, H., Yoon, J. and Lee, E.K., 2016. Oleic acid-embedded nanoliposome as a selective tumoricidal agent. Colloids and Surfaces B: Biointerfaces 146: 585-589. https://doi.org/10.1016/j.colsurfb.2016.06.058
-
Khan, H.U., Aamir, K., Jusuf, P.R., Sethi, G., Sisinthy, S.P., Ghildyal, R. and Arya, A., 2021. Lauric acid ameliorates lipopolysaccharide (LPS)-induced liver inflammation by mediating TLR4/MyD88 pathway in Sprague Dawley (SD) rats. Life Sciences 265: 118750. https://doi.org/10.1016/j.lfs.2020.118750
-
Larouche, J., Deschamps, M.H., Saucier, L., Lebeuf, Y., Doyen, A. and Vandenberg, G.W., 2019. Effects of killing methods on lipid oxidation, colour and microbial load of black soldier fly (Hermetia illucens) larvae. Animals 9: 182. https://doi.org/10.3390/ani9040182
-
Li, X., Dong, Y., Sun, Q., Tan, X., You, C., Huang, Y. and Zhou, M., 2022. Growth and fatty acid composition of black soldier fly Hermetia illucens (Diptera: Stratiomyidae) larvae are influenced by dietary fat sources and levels. Animals 12: 486. https://doi.org/10.3390/ani12040486
-
Liang, C., Gao, W., Ge, T., Tan, X., Wang, J., Liu, H., Wang, Y., Han, C., Xu, Q. and Wang, Q., 2021. Lauric acid is a potent biological control agent that damages the cell membrane of Phytophthora sojae. Frontiers in Microbiology 12: 666761. https://doi.org/10.3389/fmicb.2021.666761
-
Liu, X., Chen, X., Wang, H., Yang, Q., ur Rehman, K., Li, W., Cai, M., Li, Q., Massa, L., Zhang, J., Yu, Z. and Zheng, L., 2017. Dynamic changes of nutrient composition throughout the entire life cycle of black soldier fly. PLOS ONE 12: e0182601. https://doi.org/10.1371/journal.pone.0182601
-
Lu, Y., Zhang, S., Sun, S., Wu, M., Bao, Y., Tong, H., Ren, M., Jin, N., Xu, J., Zhou, H. and Xu, W., 2021. Effects of different nitrogen sources and ratios to carbon on larval development and bioconversion efficiency in food waste treatment by black soldier fly larvae (Hermetia illucens). Insects 12: 507. https://doi.org/10.3390/insects12060507
-
Mohan, K., Sathishkumar, P., Rajan, D.K., Rajarajeswaran, J. and Ganesan, A.R., 2023. Black soldier fly (Hermetia illucens) larvae as potential feedstock for the biodiesel production: recent advances and challenges. Science of the Total Environment 859: 160235. https://doi.org/10.1016/j.scitotenv.2022.160235
-
Mouithys-Mickalad, A., Tome, N.M., Boogaard, T., Serteyn, D., Schmitt, E. and Paul, A., 2021. Evaluation of the fat oxidation quality of commercial Hermetia illucens meal. Journal of Insects as Food and Feed 7: 965-974. https://doi.org/10.3920/JIFF2021.0001
-
Oonincx, D.G., Van Broekhoven, S., Van Huis, A. and van Loon, J.J., 2015. Feed conversion, survival and development, and composition of four insect species on diets composed of food by-products. PLOS ONE 10: e0144601. https://doi.org/10.1371/journal.pone.0222043
-
SAS, 2002. Statistical Analyses System. SAS/STAT Software, Version 9. SAS Institute Inc., Cary, NC, USA.
-
Saviane, A., Tassoni, L., Naviglio, D., Lupi, D., Savoldelli, S., Bianchi, G., Cortellino, G., Bondioli, P., Folegatti, L., Casartelli, M., Orlandi, V.T., Tettamanti, G. and Cappellozza, S., 2021. Mechanical processing of Hermetia illucens larvae and Bombyx mori pupae produces oils with antimicrobial activity. Animals 11: 783. https://doi.org/10.3390/ani11030783
-
Scieuzo, C., Giglio, F., Rinaldi, R., Lekka, M.E., Cozzolino, F., Monaco, V., Monti, M., Salvia, R. and Falabella, P., 2023. In vitro evaluation of the antibacterial activity of the peptide fractions extracted from the hemolymph of Hermetia illucens (Diptera: Stratiomyidae). Insects 14: 464. https://doi.org/10.3390/insects14050464
-
Secci, G., Addeo, N.F., Pulido Rodriguez, L.F., Bovera, F., Moniello, G. and Parisi, G., 2021. In vivo performances, ileal digestibility, and physicochemical characterisation of raw and boiled eggs as affected by Tenebrio molitor larvae meal at low inclusion rate in laying quail (Coturnix japonica) diet. Poultry Science 100: 101487.
-
Sleeman, J.P., 2000. The lymph node as a bridgehead in the metastatic dissemination of tumors. Lymphatic Metastasis and Sentinel Lymphonodectomy 157: 55-81. https://doi.org/10.1007/978-3-642-57151-0
-
Spranghers, T., Ottoboni, M., Klootwijk, C., Ovyn, A., Deboosere, S., De Meulenaer, B., Michiels, J., Eechout, M., De Clerc, P. and De Smet, S., 2017. Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. Journal of the Science of Food and Agriculture 97: 2594-2600. https://doi.org/10.1002/jsfa.8081
-
Srinivasan, S.R., Bao, W., Wattigney, W.A. and Berenson, G.S., 1996. Adolescent overweight is associated with adult overweight and related multiple cardiovascular risk factors: the bogalusa heart study. Metabolism 45: 235-240. https://doi.org/10.1016/S0026-0495(96)90060-8
-
Truzzi, C., Giorgini, E., Annibaldi, A., Antonucci, M., Illuminati, S., Scarponi, G., Riolo, P., Isidoro, N., Conti, C., Zarantoniello, M., Cipriani, R. and Olivotto, I., 2020. Fatty acids profile of black soldier fly (Hermetia illucens): influence of feeding substrate based on coffee-waste silverskin enriched with microalgae. Animal Feed Science and Technology 259: 114309. https://doi.org/10.1016/j.anifeedsci.2019.114309
-
Vandeweyer, D., Lachi, D., Van Campenhout, L. and Van Der Borght, M., 2022. Comparison of fat oxidation during frozen or nitrogen atmosphere storage of dried black soldier fly larvae (Hermetia illucens). In: insects to Feed the World 2022, 12-16 June 2022, Québec City, Québec, Canada.
-
Vyncke, W., 1975. Evaluation of the direct thiobarbituric acid extraction method for determining oxidative rancidity in mackerel (Scomber scombrus L.). Fette, Seifen, Anstrichmittel 77: 239-240. https://doi.org/10.1002/lipi.19750770610
-
Wedwitschka, H., Gallegos Ibanez, D. and Jáquez, D.R., 2023. Biogas production from residues of industrial insect protein production from black soldier fly larvae Hermetia illucens (L.): an evaluation of different insect frass samples. Processes 11: 362. https://doi.org/10.3390/pr11020362
-
Zarantoniello, M., Zimbelli, A., Randazzo, B., Compagni, M.D., Truzzi, C., Antonucci, M., Riolo, P., Loreto, N., Osimani, A., Milanovic, V., Giorgini, E., Cardinaletti, G., Tulli, F., Cipriani, R., Gioacchini, G. and Olivotto, I., 2020. Black soldier fly (Hermetia illucens) reared on roasted coffee by-product and Schizochytrium sp. as a sustainable terrestrial ingredient for aquafeeds production. Aquaculture 518: 734659. https://doi.org/10.1016/j.aquaculture.2019.734659
-
Zhu, Z., Rehman, K.U., Yu, Y., Liu, X., Wang, H., Tomberlin, J.K., Sze, S.H., Cai, M., Zhang, J., Yu, Z., Zheng, J. and Zheng, L., 2019. De novo transcriptome sequencing and analysis revealed the molecular basis of rapid fat accumulation by black soldier fly (Hermetia illucens, L.) for development of insectival biodiesel. Biotechnology for Biofuels 12: 1-16. https://doi.org/10.1186/s13068-019-1531-7
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Fatty acid profile of black soldier fly larvae and frass as affected by different growing substrates
In: Journal of Insects as Food and FeedSearch for other papers by N.F. Addeo in
Current site
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Search for other papers by I. Tucciarone in
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PubMed
Search for other papers by F. Bovera in
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PubMed
Search for other papers by S. Vozzo in
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PubMed
Search for other papers by G. Secci in
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Search for other papers by G. Parisi in
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Abstract
This study aimed to evaluate how different food wastes used as growth substrates affected the lipid composition of Hermetia illucens larvae; the relationship among substrates, larvae, and frass fatty acid (FA) composition was studied. Six thousand five-day-old Hermetia illucens larvae were allotted to one of four different substrates named CTRL (a control substrate made of broiler feed), V50 + B50 (vegetable and butchery wastes, 1:1 ratio), V75 + B25 (vegetable and butchery wastes, 3:1 ratio), and V100 (entirely composed of vegetables). Lipids were extracted from substrates, larvae, and frass separately and the FAs were quantified. Saturated FAs (SFAs) prevailed in all the groups. The CTRL larvae had the highest SFA (64.3%), while the V100 and V50 + B50 larvae had the lowest percentages. The V100 frass recorded a significantly higher SFA value (44.8%) than the others. Lauric acid (C12:0) was high in CTRL (0.2%) and V100 (0.3%) substrates and amount at 7.6 and 9.6% in their correspondent frass, respectively. However, C12:0 content was higher in the CTRL larvae than in the V100 ones (36.7 and 24.5%, respectively), while it had an intermediate value (28.9%) in the V75 + B25 larvae. Finally, the n-3 polyunsaturated FAs (PUFAs) level was high in the V100 substrate and larvae but not in the V100 frass. The relationship between frass FAs and their correspondent amounts in substrates and larvae was significant for C12:0, C18:2n-6, MUFAs, and n-6 PUFAs. There was a positive relationship for C12:0 and MUFAs with both substrates and larvae, while for C18:2n-6 and n-6 PUFAs, the relationship was positive for substrates but negative for larvae. In conclusion, the V100 substrate appeared to be the most suitable treatment for rearing Hermetia illucens because of the positive effects on the fatty acid content of larvae (low SFA and high n-3 PUFA content) and frass (optimal lauric acid levels for fertilizers).
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 782 | 338 | 51 |
| Full Text Views | 34 | 22 | 0 |
| PDF Views & Downloads | 85 | 51 | 0 |
